Construct: ORF ccsbBroad304_07304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018032.1_s304c1, BRDN0000400048
- Derived from:
- ccsbBroadEn_07304
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF10B (8795)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07304
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8795 | TNFRSF10B | TNF receptor superfamily me... | NM_003842.5 | 99.8% | 99.5% | 95C>T;572T>C |
| 2 | human | 8795 | TNFRSF10B | TNF receptor superfamily me... | NM_147187.3 | 93.3% | 93.1% | 95C>T;546_547ins87 |
| 3 | human | 8795 | TNFRSF10B | TNF receptor superfamily me... | NR_027140.2 | 28.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga acaacgggga cagaacgccc cggccgcttc gggggcccgg aaaaggcacg 121 gcccaggacc cagggaggcg cggggagcca ggcctgggct ccgggtcccc aagacccttg 181 tgctcgttgt cgccgcggtc ctgctgttgg tctcagctga gtctgctctg atcacccaac 241 aagacctagc tccccagcag agagcggccc cacaacaaaa gaggtccagc ccctcagagg 301 gattgtgtcc acctggacac catatctcag aagacggtag agattgcatc tcctgcaaat 361 atggacagga ctatagcact cactggaatg acctcctttt ctgcttgcgc tgcaccaggt 421 gtgattcagg tgaagtggag ctaagtccct gcaccacgac cagaaacaca gtgtgtcagt 481 gcgaagaagg caccttccgg gaagaagatt ctcctgagat gtgccggaag tgccgcacag 541 ggtgtcccag agggatggtc aaggtcggtg attgtacacc ctggagtgac atcgaatgtg 601 tccacaaaga atcaggtaca aagcacagtg gggaagcccc agctgtggag gagacggtga 661 cctccagccc agggactcct gcctctccct gttctctctc aggcatcatc ataggagtca 721 cagttgcagc cgtagtcttg attgtggctg tgtttgtttg caagtcttta ctgtggaaga 781 aagtccttcc ttacctgaaa ggcatctgct caggtggtgg tggggaccct gagcgtgtgg 841 acagaagctc acaacgacct ggggctgagg acaatgtcct caatgagatc gtgagtatct 901 tgcagcccac ccaggtccct gagcaggaaa tggaagtcca ggagccagca gagccaacag 961 gtgtcaacat gttgtccccc ggggagtcag agcatctgct ggaaccggca gaagctgaaa 1021 ggtctcagag gaggaggctg ctggttccag caaatgaagg tgatcccact gagactctga 1081 gacagtgctt cgatgacttt gcagacttgg tgccctttga ctcctgggag ccgctcatga 1141 ggaagttggg cctcatggac aatgagataa aggtggctaa agctgaggca gcgggccaca 1201 gggacacctt gtacacgatg ctgataaagt gggtcaacaa aaccgggcga gatgcctctg 1261 tccacaccct gctggatgcc ttggagacgc tgggagagag acttgccaag cagaagattg 1321 aggaccactt gttgagctct ggaaagttca tgtatctaga aggtaatgca gactctgcca 1381 tgtcctaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1441 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1501 acctctggat ta