Construct: ORF ccsbBroad304_07487

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002316.1_s304c1, BRDN0000462422
Derived from:
ccsbBroadEn_07487
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BMS1 (9790)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07487
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9790 BMS1 BMS1 ribosome biogenesis fa... NM_014753.4 99.9% 99.9% 336T>C;2445C>T;3421G>A
2 human 9790 BMS1 BMS1 ribosome biogenesis fa... XM_005271846.3 99.9% 99.9% 336T>C;2445C>T;3421G>A
3 human 9790 BMS1 BMS1 ribosome biogenesis fa... XM_011540402.2 99.9% 99.9% 336T>C;2445C>T;3421G>A
4 human 9790 BMS1 BMS1 ribosome biogenesis fa... XM_005271848.3 95% 95% (many diffs)
5 human 9790 BMS1 BMS1 ribosome biogenesis fa... XR_002957049.1 89.7% (many diffs)
6 human 9790 BMS1 BMS1 ribosome biogenesis fa... XR_001747266.1 87.7% (many diffs)
7 human 9790 BMS1 BMS1 ribosome biogenesis fa... XR_246522.1 87% (many diffs)
8 human 9790 BMS1 BMS1 ribosome biogenesis fa... XR_428728.2 68.9% (many diffs)
9 human 9790 BMS1 BMS1 ribosome biogenesis fa... XM_011540403.2 67.8% 67.8% 0_1ins1233;1212C>T;2188G>A
10 human 101929959 LOC101929959 ribosome biogenesis protein... XR_002956837.1 33.3% (many diffs)
11 human 101929959 LOC101929959 ribosome biogenesis protein... XM_017015376.1 20.5% 17.1% (many diffs)
12 human 101929959 LOC101929959 ribosome biogenesis protein... XM_017015377.1 20.5% 17.1% (many diffs)
13 human 728034 BMS1P14 BMS1 pseudogene 14 NR_146106.1 18.9% (many diffs)
14 human 728034 BMS1P14 BMS1 pseudogene 14 NR_146108.1 17.1% (many diffs)
15 human 728034 BMS1P14 BMS1 pseudogene 14 NR_146105.1 16.6% (many diffs)
16 human 728034 BMS1P14 BMS1 pseudogene 14 NR_146109.1 16.3% (many diffs)
17 human 728034 BMS1P14 BMS1 pseudogene 14 NR_146110.1 15.8% (many diffs)
18 human 728034 BMS1P14 BMS1 pseudogene 14 NR_146107.1 15.3% (many diffs)
19 human 101929959 LOC101929959 ribosome biogenesis protein... XM_017015378.1 14.9% 10.1% (many diffs)
20 human 101929959 LOC101929959 ribosome biogenesis protein... XM_017015379.1 14.2% 9.8% (many diffs)
21 human 101929959 LOC101929959 ribosome biogenesis protein... XM_011518094.2 11.4% 11.1% (many diffs)
22 mouse 213895 Bms1 BMS1, ribosome biogenesis f... NM_194339.1 83.6% 86.2% (many diffs)
23 mouse 213895 Bms1 BMS1, ribosome biogenesis f... XM_017321497.1 82% 84.4% (many diffs)
24 mouse 213895 Bms1 BMS1, ribosome biogenesis f... XR_377435.3 65.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3915
ORF length:
3846
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaggctaag gaccagaaga aacacagaaa gaaaaacagt ggacccaaag
121ctgcaaagaa aaagaagcgg cttctgcagg atctccagct aggagacgaa gaagatgccc
181ggaagagaaa tcccaaagct tttgcagttc agtctgctgt gcggatggct cgatcctttc
241acaggactca ggatttgaag acaaaaaagc atcatattcc agtggttgat cgaactccac
301tagagccccc accaatagtg gtagtggtga tgggacctcc aaaagttgga aagagcactt
361tgatacaatg cctcattcgg aactttaccc ggcagaagtt gaccgagatc agaggccctg
421tgacgattgt gtcaggtaaa aagcgcagac tcaccattat tgaatgtggg tgtgacatta
481acatgatgat tgatctggct aaagtagcag atctggtact gatgcttata gatgccagct
541ttgggtttga aatggaaacg tttgagtttc taaacatctg tcaagtacat ggctttccta
601aaattatggg agttctcacc cacctcgact ccttcaagca taataagcaa ctgaagaaga
661caaagaagcg attaaaacac aggttctgga cggaagttta cccgggtgcc aagctgttct
721acctttctgg aatggtgcat ggagaatatc aaaaccaaga aatccacaat ctgggccgtt
781ttattacagt tatgaagttt aggcctctca catggcaaac ttctcaccct tatatcctgg
841cagacaggat ggaagatttg acaaacccag aggatatccg aacaaacatc aaatgtgacc
901ggaaggtgtc actttatggt tatttaagag gagcacactt gaaaaataaa agccaaattc
961acatgccagg ggtaggagat tttgccgtga gtgacatcag tttcctccca gacccttgcg
1021ctcttcctga acaacaaaag aagcgctgtt taaatgagaa ggagaagctg gtttatgcgc
1081ctctttctgg agttgggggt gtgctgtatg acaaagacgc tgtctatgtt gaccttggtg
1141gcagccacgt ttttcaggat gaagtggggc ccacccatga gctggtccag agtctcatct
1201ctacccactc caccattgat gccaagatgg cttcaagtcg agtgacgctg ttttctgatt
1261ccaagccact tgggtcagag gatatagata atcaagggct aatgatgcca aaggaggaaa
1321aacaaatgga cttgaacact ggtcgaatgc gtcggaaagc cattttcgga gatgaagatg
1381aatctggaga tagtgatgat gaagaagatg atgaaatgtc tgaagatgac gggttggaaa
1441acggctctag tgatgaggaa gcagaagagg aggaaaatgc tgagatgact gatcagtata
1501tggctgttaa gggcatcaaa cgacggaaac ttgagttgga agaagacagt gaaatggatt
1561tgccagcatt tgctgacagt gacgatgacc ttgagaggag ctcagcggaa gaaggggaag
1621cggaggaagc tgatgaaagc agtgaagaag aggactgcac tgcaggagag aagggcattt
1681caggatcaaa ggctgctgga gaaggtagta aagcagggct gtcaccagct aattgccaga
1741gtgaccgtgt gaatctggag aagtctttgc tgatgaagaa agcagctctc cccactttcg
1801attctgggca ttgcacagct gaagaggtgt ttgcatctga agatgaatct gaagaaagct
1861cctcactcag tgcagaggaa gaagactcag aaaatgaaga ggctattaga aaaaagcttt
1921caaagccttc tcaagtgagc agtggtcaga aactggggcc acagaacttc attgatgaga
1981ccagtgatat agaaaattta ctcaaagagg aagaagatta caaggaagaa aataatgatt
2041ccaaagaaac gtcaggtgcc ctcaagtgga aggaagacct ttccagaaag gcagctgagg
2101cctttctgag gcagcagcaa gcagctccaa acctccgaaa gcttatttat gggacagtga
2161cagaagataa tgaagaagaa gatgatgata ctctagaaga gcttggaggg ttgtttcgtg
2221tcaaccagcc tgacagagag tgtaagcaca aggctgactc tttggactgc tccagatttc
2281ttgtggaggc cccccatgac tgggatttag aggaggttat gaacagtatc agagattgct
2341tcgtgactgg aaagtgggaa gatgataaag atgcagccaa ggtcttagca gaagatgagg
2401agctctacgg tgactttgaa gacttggaaa caggggacgt gcacaaggga aaatcaggcc
2461ccaatactca gaatgaagat atagagaaag aagttaagga agaaattgac cctgacgaag
2521aagaaagtgc caagaaaaag catttggata agaagagaaa attgaaggag atgtttgatg
2581cagaatatga tgaaggagaa agcacatatt ttgatgatct taaaggagaa atgcagaaac
2641aagcacagct gaatcgcgca gaatttgaag atcaagatga tgaagccaga gttcagtatg
2701agggttttcg acctgggatg tacgtccgca ttgagattga aaatgttccc tgtgaatttg
2761tgcagaactt tgacccccat taccccatta tcctgggtgg cttgggcaac agtgagggaa
2821atgttggcta cgtgcagatg cgtctgaaga aacatcgctg gtataagaaa atcctcaagt
2881cccgagatcc aatcatattt tctgtagggt ggaggaggtt tcagaccatc ccactgtatt
2941atatcgaaga ccacaatgga agacaaaggc ttctaaagta taccccacag cacatgcatt
3001gcggagcagc cttttggggc cctatcactc cacagggaac tggtttcttg gcaatacagt
3061ctgtcagtgg cataatgcct gattttcgga tagctgctac aggagttgtc cttgatctgg
3121ataaatccat aaaaattgtg aagaaattaa agctaactgg ttttccatat aaaattttca
3181agaacacttc atttattaag ggaatgttta attctgcctt ggaagtggcc aaatttgaag
3241gtgctgtgat tcgaacagtc agtgggataa gggggcagat caagaaagca ctccgagctc
3301cagaaggagc tttcagggcc agctttgagg ataagctgct gatgagcgat attgtcttca
3361tgcgaacttg gtatcctgtt tccatcccag cgttctataa cccagtaaca tctttgttga
3421aaccagtggg tgagaaagac acctggtcag gaatgcggac cacgggccaa ctcaggctcg
3481cccatggcat cagactaaag gcgaacaagg actctctgta taagccaatc ctgaggcaaa
3541agaaacattt taattcactg cacattccaa aagccttgca gaaggccctg ccatttaaga
3601acaagcccaa gacccaagca aaggcaggca aggtgccaaa ggacaggcgg agaccggccg
3661tcatacgcga gcctcatgaa agaaagatcc ttgcactgct ggatgctctg agtacggtgc
3721atagtcagaa gatgaagaag gccaaggagc agcggcacct gcacaataaa gagcacttca
3781gagccaagca gaaggaggag gaggagaagc tgaagcggca gaaggacctc aggaagaagc
3841tcttcagaat tcaggggcag aaggaaagaa gaaaccagaa gtccagtttg aagggggctg
3901agggccaatt gcagttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
3961ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
4021cgacaatcaa cctctggatt a

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