Construct: ORF ccsbBroad304_07499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007112.1_s304c1, BRDN0000409066
- Derived from:
- ccsbBroadEn_07499
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MED24 (9862)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07499
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9862 | MED24 | mediator complex subunit 24 | NM_014815.4 | 99.9% | 100% | 1161G>A;2886C>T |
| 2 | human | 9862 | MED24 | mediator complex subunit 24 | NM_001079518.2 | 98.6% | 98.6% | 211_212ins39;1122G>A;2847C>T |
| 3 | human | 9862 | MED24 | mediator complex subunit 24 | NM_001267797.2 | 98.6% | 98.6% | 211_212ins39;1122G>A;2847C>T |
| 4 | human | 9862 | MED24 | mediator complex subunit 24 | NM_001330211.2 | 98% | 98.1% | 560_616del;1218G>A;2943C>T |
| 5 | human | 9862 | MED24 | mediator complex subunit 24 | NR_052017.2 | 83% | (many diffs) | |
| 6 | mouse | 23989 | Med24 | mediator complex subunit 24 | NM_011869.2 | 88.7% | 94.2% | (many diffs) |
| 7 | mouse | 23989 | Med24 | mediator complex subunit 24 | XM_006533305.2 | 88.7% | 94.2% | (many diffs) |
| 8 | mouse | 23989 | Med24 | mediator complex subunit 24 | XM_006533302.1 | 87% | 92.4% | (many diffs) |
| 9 | mouse | 23989 | Med24 | mediator complex subunit 24 | XM_006533303.2 | 87% | 92.4% | (many diffs) |
| 10 | mouse | 23989 | Med24 | mediator complex subunit 24 | XM_006533304.2 | 87% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3033
- ORF length:
- 2967
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggtggtcaac ctgaagcaag ccattttgca agcctggaag gagcgctgga 121 gtgactacca atgggcaatc aacatgaaga aattctttcc taaaggagcc acctgggata 181 ttctcaacct ggcagatgcg ttactagagc aggccatgat tggaccatcc cccaatcctc 241 tcatcttgtc ctacctgaag tatgccatta gttcccagat ggtgtcctac tcttctgtcc 301 tcacagccat cagtaagttt gatgactttt ctcgggacct gtgtgtccag gcattgctgg 361 acatcatgga catgttttgt gaccgtctga gctgtcacgg caaagcagag gaatgcatcg 421 gactgtgccg agcccttctt agcgccctcc actggctgct gcgctgcacg gcagcctctg 481 cagagcggct gcgggagggg ctggaggccg gcactccagc cgctggggag aagcagcttg 541 ccatgtgcct tcagcgcctg gagaaaaccc tcagcagcac caagaaccgg gccctgctgc 601 acatcgccaa actagaggag gcctcttctt ggactgccat cgagcattct ctcttgaaac 661 ttggagagat cctggccaat ctcagcaacc cgcagctccg gagtcaggcc gagcagtgtg 721 gcaccctcat taggagcatc cccacgatgc tgtctgtgca tgcggagcag atgcacaaga 781 ccggcttccc cactgtccac gccgtgatcc tgctcgaggg caccatgaac ctgacaggcg 841 agacgcagtc cctggtggag cagctgacga tggtgaagcg catgcagcat atccccaccc 901 cactttttgt cctggagatc tggaaagctt gcttcgtggg gctcattgag tctcccgagg 961 gtacggagga gctcaagtgg acagctttca ctttcctcaa gattccacag gttttggtga 1021 agttgaagaa gtactctcat ggagacaagg acttcactga ggatgtcaac tgtgcttttg 1081 agttcctgct gaagctcacc cccttgttgg acaaagctga ccagcgctgc aactgtgact 1141 gtacaaactt cctgctccaa gaatgtggca agcaggggct tctgtctgag gccagcgtca 1201 acaaccttat ggctaagcgc aaagcagacc gagagcacgc accccagcag aaatcgggag 1261 agaatgccaa catccagccc aacatccagc tgatcctccg ggcggagccc actgtcacaa 1321 acatcctcaa gacgatggat gcagaccact ctaagtcacc ggagggactg ctgggagtcc 1381 tgggccacat gctgtccggg aagagtctgg acttgctgct ggctgccgcc gccgccactg 1441 gaaagctgaa atccttcgcc cggaaattca tcaatttgaa tgaattcaca acctatggca 1501 gcgaagaaag caccaaaccg gcctccgtcc gggccctgct gtttgacatc tccttcctca 1561 tgctgtgcca tgtggcccag acctatggtt cagaggtgat tctgtccgag tcgcgcacag 1621 gagctgaggt gcccttcttc gagacctgga tgcagacctg catgcctgag gagggcaaga 1681 tcctgaaccc tgaccacccc tgcttccgcc ccgactccac caaagtggag tccctggtgg 1741 ccctgctcaa caactcctcg gagatgaagc tagtgcagat gaagtggcat gaggcctgtc 1801 tcagcatctc agccgccatc ttggaaatcc tcaatgcctg ggagaatggg gtcctggcct 1861 tcgagtccat ccagaaaatc actgataaca tcaaagggaa ggtatgcagt ctggcggtgt 1921 gtgctgtggc ttggcttgtg gcccacgtcc ggatgctggg gctggatgag cgtgagaagt 1981 cgctgcagat gatccgccag ctggcagggc cactgtttag tgagaacacc ctgcagttct 2041 acaatgagag ggtggtgatc atgaactcga tcctggagcg catgtgtgcc gacgtgctgc 2101 agcagacagc cacgcagatc aagtttccct ccaccggggt ggacacaatg ccctactgga 2161 acctgctgcc ccccaagcgg cccatcaaag aggtgctgac ggacattttt gccaaggtgc 2221 tggagaaggg ctgggtggac agccgctcca tccacatctt tgacaccctg ctgcacatgg 2281 gcggcgtcta ctggttctgc aacaacctga ttaaggagct gctgaaggag acgcggaagg 2341 agcacacgct gcgggcagtg gagctgctct actccatctt ctgcctggac atgcagcaag 2401 tgaccctggt cctgctgggc cacatcctac ctggcctgct cactgactcc tccaagtggc 2461 acagcctcat ggaccccccg ggcactgctc ttgccaagct ggccgtgtgg tgtgccctca 2521 gttcctactc ctcccacaag ggacaggcgt ccacccgcca gaagaagaga caccgcgaag 2581 acattgagga ttatatcagc ctcttccccc tggacgatgt gcagccttcg aagttgatgc 2641 gactgctgag ctctaatgag gacgatgcca acatcctttc gagccccaca gaccgatcca 2701 tgagcagctc cctctcagcc tctcagctcc acacggtcaa catgcgggac cctctgaacc 2761 gagtcctggc caacctgttc ctgctcatct cctccatcct ggggtctcgc accgctggcc 2821 cccacaccca gttcgtgcag tggttcatgg aggagtgtgt ggactgcctg gagcagggtg 2881 gccgtggcag cgtcctgcag ttcatgccct tcaccaccgt gtcggaactg gtgaaggtgt 2941 cagccatgtc tagccccaag gtggttctgg ccatcacgga cctcagcctg cccctgggcc 3001 gccaggtggc tgctaaagcc attgctgcac tctgcccaac tttcttgtac aaagtggttg 3061 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 3121 accggtggcg cgttaagtcg acaatcaacc tctggatta