Construct: ORF ccsbBroad304_07520

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018248.1_s304c1, BRDN0000401810
Derived from:
ccsbBroadEn_07520
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP15 (9958)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07520
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9958 USP15 ubiquitin specific peptidas... NM_006313.3 99.9% 99.8% 2153C>T;2214T>C
2 human 9958 USP15 ubiquitin specific peptidas... NM_001252078.2 96.9% 96.9% 682_768del;2240C>T;2301T>C
3 human 9958 USP15 ubiquitin specific peptidas... NM_001351159.2 84.6% 84.6% (many diffs)
4 human 9958 USP15 ubiquitin specific peptidas... NM_001351160.2 76.8% 76% (many diffs)
5 human 9958 USP15 ubiquitin specific peptidas... NM_001351163.2 67.9% 67.9% 0_1ins912;1241C>T;1302T>C
6 human 9958 USP15 ubiquitin specific peptidas... NM_001351164.2 67.9% 67.9% 0_1ins912;1241C>T;1302T>C
7 human 9958 USP15 ubiquitin specific peptidas... NM_001351165.2 67.9% 67.9% 0_1ins912;1241C>T;1302T>C
8 human 9958 USP15 ubiquitin specific peptidas... NM_001351166.2 67.9% 67.9% 0_1ins912;1241C>T;1302T>C
9 human 9958 USP15 ubiquitin specific peptidas... NM_001252079.1 24.2% 23.9% (many diffs)
10 human 9958 USP15 ubiquitin specific peptidas... NR_147081.2 19.1% (many diffs)
11 human 9958 USP15 ubiquitin specific peptidas... NR_147078.2 19% (many diffs)
12 human 9958 USP15 ubiquitin specific peptidas... NR_147079.2 19% (many diffs)
13 human 9958 USP15 ubiquitin specific peptidas... NR_147082.2 18.9% (many diffs)
14 human 9958 USP15 ubiquitin specific peptidas... NR_147080.2 18.7% (many diffs)
15 human 9958 USP15 ubiquitin specific peptidas... NM_001351161.2 12.6% 12.1% (many diffs)
16 human 9958 USP15 ubiquitin specific peptidas... NM_001351162.1 8.3% 7.9% (many diffs)
17 human 102465133 MIR6125 microRNA 6125 NR_106740.1 1.3% 1_56del;96_97ins2816
18 mouse 14479 Usp15 ubiquitin specific peptidas... NM_001301628.1 90.1% 97.5% (many diffs)
19 mouse 14479 Usp15 ubiquitin specific peptidas... NM_027604.4 87.5% 94.6% (many diffs)
20 mouse 14479 Usp15 ubiquitin specific peptidas... XM_006513225.3 68.7% 74.2% (many diffs)
21 mouse 14479 Usp15 ubiquitin specific peptidas... XM_006513226.3 68.4% 73.8% (many diffs)
22 mouse 14479 Usp15 ubiquitin specific peptidas... XM_006513227.3 63.9% 69.4% (many diffs)
23 mouse 14479 Usp15 ubiquitin specific peptidas... XR_380365.3 27.6% (many diffs)
24 mouse 14479 Usp15 ubiquitin specific peptidas... XM_006513228.3 25.1% 23.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2925
ORF length:
2856
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggaaggc ggagcggcgg atctggacac ccagcggtct gacatcgcga
121cgctgctcaa aacctcgctc cggaaagggg acacctggta cctagtcgat agtcgctggt
181tcaaacagtg gaaaaaatat gttggctttg acagttggga caaataccag atgggagatc
241aaaatgtgta tcctggaccc attgataact ctggacttct caaagatggt gatgcccagt
301cacttaagga acaccttatt gatgaattgg attacatact gttgccaact gaaggttgga
361ataaacttgt cagctggtac acattgatgg aaggtcaaga gccaatagca cgaaaggtgg
421ttgaacaggg tatgtttgta aagcactgca aagtagaagt atatctcaca gaattgaagc
481tatgtgaaaa tggaaacatg aataatgttg taactcgaag atttagcaaa gctgacacaa
541tagatacaat tgaaaaggaa ataagaaaaa tcttcagtat tccagatgaa aaggagacca
601gattgtggaa caaatacatg agtaacacat ttgaaccact gaataaacca gacagcacca
661ttcaggatgc tggtttatac caaggacagg tattagtgat agaacagaaa aatgaagatg
721gaacatggcc aaggggtcct tctactccta atgtgaaaaa ctcaaattac tgtcttccat
781catataccgc ttataagaac tatgattatt cggaacctgg aagaaacaat gaacagccag
841gcctctgtgg cctaagtaac ttgggaaata cgtgtttcat gaactcagct attcagtgtt
901tgagcaacac acctccactt actgagtatt tcctcaatga taagtatcaa gaagaactga
961attttgacaa tcccttagga atgagaggtg aaatagctaa atcttatgcc gaactgatca
1021agcaaatgtg gtctggaaag tttagctacg tcaccccaag agcctttaag acacaggtag
1081gacgttttgc acctcagttc tctggatatc agcagcaaga ctgtcaagaa ctgttagctt
1141tcctattaga tggattacat gaggatttga atagaattag gaaaaaacca tatatacaat
1201taaaagatgc agatggaagg ccagataagg tggttgccga agaagcctgg gaaaaccatt
1261taaaacgaaa tgattctatc atagtagata tatttcatgg ccttttcaaa tcaactttag
1321tttgtcctga gtgtgctaag atttcagtaa catttgatcc tttttgttac ttgacacttc
1381cattgcccat gaaaaaagaa cgcaccttgg aagtttactt agttagaatg gatccactta
1441ccaaacctat gcagtacaaa gtggttgtcc ccaaaattgg aaacatatta gatctttgta
1501cagcattgtc tgctttgtca ggaatacctg cagataagat gatagttact gatatataca
1561atcatagatt tcacagaata ttcgctatgg atgaaaacct tagtagtatt atggaacggg
1621atgatattta tgtgtttgaa attaacatca ataggacaga agatacagag cacgtgatta
1681ttcctgtttg cctaagagaa aaattcagac actcgagtta tacccaccat actggttctt
1741cactttttgg tcagcccttt cttatggctg taccacgaaa caatactgaa gacaaacttt
1801ataatctcct gctcttgaga atgtgccgat atgtcaaaat atctactgaa actgaagaaa
1861ctgaaggatc cctacactgc tgtaaggacc aaaatattaa tgggaatggc ccaaatggca
1921tacatgaaga aggctcacca agtgaaatgg aaacagatga gccagatgat gaatccagcc
1981aggatcaaga acttccctca gagaatgaaa acagtcagtc tgaagattca gttggaggag
2041ataatgattc tgaaaatgga ttatgtactg aggatacttg caaaggtcaa ctcacgggac
2101acaaaaaacg attgtttaca ttccagttca acaacttagg caatactgat atcaactaca
2161tcaaagatga taccaggcat ataagatttg atgataggca gcttaggcta gatgaaagat
2221tttttcttgc tttggattgg gatcctgatt tgaaaaaaag atattttgat gaaaatgctg
2281ccgaggactt tgaaaaacat gaaagtgtgg agtataaacc tcctaaaaaa ccctttgtga
2341aattaaaaga ttgcattgaa ctttttacaa caaaagaaaa gctaggtgct gaagatccct
2401ggtattgtcc gaattgtaaa gaacatcagc aagccacaaa gaaattggat ttatggtccc
2461tgcctccagt acttgtagta catctcaagc gattttctta cagtcgatac atgagagaca
2521agttggatac cttagttgat tttcctatca atgacttgga tatgtcggaa ttcttaatta
2581atccaaatgc aggtccttgc cgctataatc tgattgctgt ttccaaccac tatggaggga
2641tgggaggagg acactatact gcttttgcaa aaaataaaga tgatggaaaa tggtactatt
2701ttgatgacag tagtgtctcc actgcatctg aagaccaaat tgtgtccaaa gcagcatatg
2761tactcttcta ccagagacaa gacactttca gtggaactgg cttttttcct cttgaccgag
2821aaactaaagg tgcttcagct gccactggca tcccattaga aagtgatgaa gatagcaatg
2881ataatgacaa tgatatagaa aatgaaaact gtatgcacac taacttgcca actttcttgt
2941acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
3001gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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