Construct: ORF ccsbBroad304_07565

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF010499.1_s304c1, BRDN0000400866
• Derived from: ccsbBroadEn_07565
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: RBM6 (10180)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_07565
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	10180	RBM6	RNA binding motif protein 6	NM_001167582.2	99.9%	100%	1755C>T
2	human	10180	RBM6	RNA binding motif protein 6	NM_001349190.2	99.9%	100%	1755C>T
3	human	10180	RBM6	RNA binding motif protein 6	NM_001349191.2	99.9%	100%	1755C>T
4	human	10180	RBM6	RNA binding motif protein 6	NM_001349192.2	99.9%	100%	1755C>T
5	human	10180	RBM6	RNA binding motif protein 6	NM_001349193.2	99.9%	100%	1755C>T
6	human	10180	RBM6	RNA binding motif protein 6	XM_017005500.2	99.9%	100%	1755C>T
7	human	10180	RBM6	RNA binding motif protein 6	XM_017005501.2	99.9%	100%	1755C>T
8	human	10180	RBM6	RNA binding motif protein 6	XM_017005502.1	99.9%	100%	1755C>T
9	human	10180	RBM6	RNA binding motif protein 6	XM_024453288.1	99.9%	100%	1755C>T
10	human	10180	RBM6	RNA binding motif protein 6	NM_001349194.2	98.6%	98.6%	1_24del;1779C>T
11	human	10180	RBM6	RNA binding motif protein 6	XM_017005497.1	94.1%	94.2%	1_111del;1866C>T
12	human	10180	RBM6	RNA binding motif protein 6	XM_005264785.1	93.5%	93.6%	1_123del;1878C>T
13	human	10180	RBM6	RNA binding motif protein 6	NR_146071.2	86.8%		(many diffs)
14	human	10180	RBM6	RNA binding motif protein 6	XM_005264784.1	60.6%	60.6%	1_1170del;2925C>T
15	human	10180	RBM6	RNA binding motif protein 6	XM_006712916.1	60.6%	60.6%	1_1170del;2925C>T
16	human	10180	RBM6	RNA binding motif protein 6	NM_005777.3	53.4%	53.5%	1_1566del;3321C>T
17	human	10180	RBM6	RNA binding motif protein 6	XR_001739975.2	49.2%		(many diffs)
18	human	10180	RBM6	RNA binding motif protein 6	XR_002959486.1	36.7%		1_1829del;3164_3165ins468
19	human	10180	RBM6	RNA binding motif protein 6	XM_017005496.2	21.2%	19.5%	(many diffs)
20	mouse	19654	Rbm6	RNA binding motif protein 6	XM_006511651.3	87.6%	89.3%	(many diffs)
21	mouse	19654	Rbm6	RNA binding motif protein 6	XM_006511653.2	87.6%	89.3%	(many diffs)
22	mouse	19654	Rbm6	RNA binding motif protein 6	XM_011242860.2	87.6%	89.3%	(many diffs)
23	mouse	19654	Rbm6	RNA binding motif protein 6	XM_017313217.1	87.6%	89.3%	(many diffs)
24	mouse	19654	Rbm6	RNA binding motif protein 6	XM_011242859.2	82.5%	84.1%	(many diffs)
25	mouse	19654	Rbm6	RNA binding motif protein 6	NM_029169.3	53.1%	54.1%	(many diffs)
26	mouse	19654	Rbm6	RNA binding motif protein 6	XM_017313216.1	53.1%	54.1%	(many diffs)
27	mouse	19654	Rbm6	RNA binding motif protein 6	NM_011251.3	46.9%	47.8%	(many diffs)
28	mouse	19654	Rbm6	RNA binding motif protein 6	XM_006511650.3	46.9%	47.8%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1869

ORF length:

1803

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgat ccaggacaaa gaagttaccc tggagtatgt atcaagcctg gatttttggt
121	actgcaaacg atgtaaggca aacattggtg ggcaccgatc ttcctgttca ttctgcaaga
181	acccaagaga agtgacagag gccaagcaag aattaataac ctaccctcag cctcagaaaa
241	catccatacc agcaccattg gaaaaacagc ccaaccagcc cctaagacca gctgataagg
301	aacctgaacc caggaagagg gaagaaggcc aagagtcacg cttaggacat caaaagagag
361	aagcagaaag gtatctgcct ccttctcgaa gggaagggcc aactttccga agagaccgag
421	agagggagtc atggtctgga gagacacgcc aggatggaga gagcaaaact atcatgctaa
481	agcgtatcta tcgttccaca ccacctgagg tgatagtgga agtgctggag ccctatgtcc
541	gccttactac tgccaacgtc cgtatcatca agaacagaac aggccctatg gggcatacct
601	atggctttat tgacctcgac tcccatgcgg aagctcttcg tgtggtgaag atcttacaga
661	accttgatcc gccatttagc attgatggga agatggtagc tgtaaacctg gccactggaa
721	aacgaagaaa tgattctggg gaccattctg accacatgca ttactatcag ggtaaaaaat
781	atttccgaga taggagggga ggtggcagaa attcagactg gtcttcagat acaaatcgac
841	aaggacaaca gtcatcatct gactgctaca tatatgattc tgctactggc tactattatg
901	accccttggc aggaacttat tatgacccca atacccagca agaagtctat gtgccccagg
961	atcctggatt acctgaggaa gaagagatca aggaaaaaaa acccaccagt caaggaaagt
1021	caagtagcaa gaaggaaatg tctaaaagag atggcaagga gaaaaaagac agaggagtga
1081	cgaggtttca ggaaaatgcc agtgaaggga aggcccctgc agaagacgtc tttaagaagc
1141	ccctgcctcc tactgtgaag aaggaagaga gtccccctcc acctaaagtg gtaaacccac
1201	tgatcggcct cttgggtgaa tatggaggag acagtgacta tgaggaggaa gaagaggagg
1261	aacagacccc tcccccacag ccccgcacag cacagcccca gaagcgagag gagcaaacca
1321	agaaggagaa tgaagaagac aaactcactg actggaataa actggcttgt ctgctttgca
1381	gaaggcagtt tcccaataaa gaagttctga tcaaacacca gcagctgtca gacctgcaca
1441	agcaaaacct ggaaatccac cggaagataa aacagtctga gcaggagcta gcctatctgg
1501	aaaggagaga acgagaggga aagtttaaag gaagaggaaa tgatcgcagg gaaaagctcc
1561	agtcttttga ctctccagaa aggaaacgga ttaagtactc cagggaaact gacagtgatc
1621	gtaaacttgt tgataaagaa gatatcgaca ctagcagcaa aggaggctgt gtccaacagg
1681	ctactggctg gaggaaaggg acaggcctgg gatatggcca tcctggattg gcttcatcag
1741	aggaggctga aggccggatg aggggcccca gtgttggagc ctcaggaaga accagcaaaa
1801	gacagtccaa cgagacttat cgagatgctg ttcgaagagt catgtttgct cgatataaag
1861	aactcgatta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1921	tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1981	tcaacctctg gatta

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