Construct: ORF ccsbBroad304_07823

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003674.1_s304c1, BRDN0000406476
Derived from:
ccsbBroadEn_07823
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKR7A3 (22977)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07823
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22977 AKR7A3 aldo-keto reductase family ... NM_012067.3 99.7% 99.6% 412G>A;939C>T
2 human 246181 AKR7L aldo-keto reductase family ... NM_001348421.1 95.5% 92.1% (many diffs)
3 human 8574 AKR7A2 aldo-keto reductase family ... NM_003689.3 83.3% 80.5% (many diffs)
4 human 22977 AKR7A3 aldo-keto reductase family ... XM_011541046.3 82.4% 73.1% (many diffs)
5 human 8574 AKR7A2 aldo-keto reductase family ... NM_001320979.1 74% 71.3% (many diffs)
6 human 22977 AKR7A3 aldo-keto reductase family ... XM_017000714.2 63.6% 54.6% (many diffs)
7 human 246182 AKR7A2P1 aldo-keto reductase family ... NR_002796.2 62.8% (many diffs)
8 human 8574 AKR7A2 aldo-keto reductase family ... XR_946785.2 59.4% (many diffs)
9 human 22977 AKR7A3 aldo-keto reductase family ... XR_001737055.1 49.1% (many diffs)
10 human 246181 AKR7L aldo-keto reductase family ... NR_040288.2 39.8% (many diffs)
11 human 246181 AKR7L aldo-keto reductase family ... NR_040289.2 32% (many diffs)
12 mouse 110198 Akr7a5 aldo-keto reductase family ... NM_025337.3 77.7% 76.2% (many diffs)
13 mouse 110198 Akr7a5 aldo-keto reductase family ... XR_390721.3 51.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1059
ORF length:
993
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggaatgtc ccggcagctg tcgcgggccc ggccagccac ggtgctgggc gccatggaga
121tggggcgccg catggacgcg cccaccagcg ccgcagtcac gcgcgccttc ctggagcgcg
181gccacaccga gatagacacg gccttcgtgt acagcgaggg ccagtccgag accatccttg
241gcggcctggg gctccggctg ggcggcagcg actgcagagt gaaaattgat accaaggcca
301ttccactgtt tgggaactcc ctgaagcctg acagtctccg gttccagctg gagacgtcac
361tgaagcggct gcagtgtccc cgagtggacc tcttctacct gcatatgcca gaccacagca
421ccccggtgga agagacactg cgtgcctgcc accagctgca ccaggagggc aagttcatgg
481agcttggcct ctccaactat gcagcctggg aagtggccga gatctgtacc ctctgcaaga
541gcaacggctg gatcctgccc actgtgtacc agggcatgta caatgccatc acccggcagg
601tggaaacgga gctcttcccc tgcctcaggc actttggact gaggttctat gccttcaacc
661ctctggctgg gggcctgctg accggcaagt acaagtatga ggacaagaat gggaaacagc
721ccgtgggccg cttctttggg aatacctggg cagagatgta caggaatcgc tactggaagg
781agcaccactt tgagggcatt gccctggtgg agaaggccct gcaggccgcg tatggcgcca
841gcgcccccag catgacctcg gccaccctcc ggtggatgta ccaccactca cagctgcagg
901gtgcccacgg ggacgcggtc atcctgggca tgtccagcct ggagcagctg gagcagaact
961tggcagcggc agaggaaggg cccctggagc cggctgtcgt ggatgccttt aatcaagcct
1021ggcatttggt tactcacgaa tgtcccaact acttccgcta cccaactttc ttgtacaaag
1081tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1141gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)