Transcript: Human NM_012067.3

Homo sapiens aldo-keto reductase family 7 member A3 (AKR7A3), mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
AKR7A3 (22977)
Length:
1215
CDS:
62..1057

Additional Resources:

NCBI RefSeq record:
NM_012067.3
NBCI Gene record:
AKR7A3 (22977)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_012067.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229386 AGATGTACAGGAATCGCTACT pLKO_005 750 CDS 100% 4.050 2.835 N AKR7A3 n/a
2 TRCN0000038990 CTCTTCTACCTGCATATGCCA pLKO.1 386 CDS 100% 0.000 0.000 N AKR7A3 n/a
3 TRCN0000229384 TACCAAGGCCATTCCACTGTT pLKO_005 286 CDS 100% 0.000 0.000 N AKR7A3 n/a
4 TRCN0000229385 GGACCTCTTCTACCTGCATAT pLKO_005 382 CDS 100% 10.800 6.480 N AKR7A3 n/a
5 TRCN0000219002 AGGCTCTTCTGTAACATCTTT pLKO_005 1082 3UTR 100% 5.625 3.375 N AKR7A3 n/a
6 TRCN0000038992 ACTGTTTGGGAACTCCCTGAA pLKO.1 301 CDS 100% 4.050 2.430 N AKR7A3 n/a
7 TRCN0000038989 AGAGATGTACAGGAATCGCTA pLKO.1 748 CDS 100% 2.640 1.584 N AKR7A3 n/a
8 TRCN0000229387 TTCCGCTAGGCCCATCGTTTC pLKO_005 1049 CDS 100% 2.000 1.200 N AKR7A3 n/a
9 TRCN0000038993 CAAGTACAAGTATGAGGACAA pLKO.1 682 CDS 100% 4.050 2.025 Y AKR7A3 n/a
10 TRCN0000038985 CCTTTAATCAAGCCTGGCATT pLKO.1 1002 CDS 100% 4.050 2.025 Y AKR7A2 n/a
11 TRCN0000046415 GCCTTTAATCAAGCCTGGCAT pLKO.1 1001 CDS 100% 2.640 1.320 Y AKR7L n/a
12 TRCN0000046417 GAGGGCAAGTTCGTGGAGCTT pLKO.1 461 CDS 100% 0.880 0.440 Y AKR7L n/a
13 TRCN0000038991 CACCGAGATAGACACGGCCTT pLKO.1 181 CDS 100% 0.720 0.360 Y AKR7A3 n/a
14 TRCN0000046416 CGGTGGATGTACCACCACTCA pLKO.1 866 CDS 100% 0.088 0.044 Y AKR7L n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_012067.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07823 pDONR223 100% 99.7% 99.6% None 412G>A;939C>T n/a
2 ccsbBroad304_07823 pLX_304 0% 99.7% 99.6% V5 412G>A;939C>T n/a
3 TRCN0000465271 GACACAACAGCATGCGGAGCAAAC pLX_317 19.9% 99.7% 99.6% V5 412G>A;939C>T n/a
4 ccsbBroadEn_07822 pDONR223 100% 99.6% 99.3% None 501G>C;643A>G;967A>G n/a
5 ccsbBroad304_07822 pLX_304 0% 99.6% 99.3% V5 501G>C;643A>G;967A>G n/a
6 TRCN0000469339 ACCTGAGCTCTACTCTGGTAGCAA pLX_317 41.9% 99.6% 99.3% V5 501G>C;643A>G;967A>G n/a
7 ccsbBroadEn_11281 pDONR223 100% 90.5% 86.5% None (many diffs) n/a
8 ccsbBroad304_11281 pLX_304 0% 90.5% 86.5% V5 (many diffs) n/a
9 TRCN0000468610 GGACTAGCCACGGGGCCGGGCTGA pLX_317 39% 90.5% 86.5% V5 (many diffs) n/a
10 ccsbBroadEn_13444 pDONR223 100% 44.9% 30.8% None (many diffs) n/a
11 ccsbBroad304_13444 pLX_304 0% 44.9% 30.8% V5 (many diffs) n/a
12 TRCN0000478142 GAGAGTCATAAAAGACCTGAGCGT pLX_317 70.7% 44.9% 30.8% V5 (many diffs) n/a
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