Transcript: Mouse NM_025337.3

Mus musculus aldo-keto reductase family 7, member A5 (aflatoxin aldehyde reductase) (Akr7a5), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Akr7a5 (110198)
Length:
1648
CDS:
12..1115

Additional Resources:

NCBI RefSeq record:
NM_025337.3
NBCI Gene record:
Akr7a5 (110198)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025337.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000042133 CGGGTGGATCTCTTCTACTTA pLKO.1 435 CDS 100% 5.625 7.875 N Akr7a5 n/a
2 TRCN0000351538 CGGGTGGATCTCTTCTACTTA pLKO_005 435 CDS 100% 5.625 7.875 N Akr7a5 n/a
3 TRCN0000042136 ACTGAGGTTCTACGCCTACAA pLKO.1 692 CDS 100% 4.950 3.960 N Akr7a5 n/a
4 TRCN0000351536 ACTGAGGTTCTACGCCTACAA pLKO_005 692 CDS 100% 4.950 3.960 N Akr7a5 n/a
5 TRCN0000042135 CCAGTTAGAGACGTCTCTGAA pLKO.1 398 CDS 100% 4.950 3.960 N Akr7a5 n/a
6 TRCN0000351537 CCAGTTAGAGACGTCTCTGAA pLKO_005 398 CDS 100% 4.950 3.960 N Akr7a5 n/a
7 TRCN0000042134 GTGTCCCAACTACTTCAGATA pLKO.1 1094 CDS 100% 4.950 3.465 N Akr7a5 n/a
8 TRCN0000323477 GTGTCCCAACTACTTCAGATA pLKO_005 1094 CDS 100% 4.950 3.465 N Akr7a5 n/a
9 TRCN0000042137 ACTGGCAAGTATAAATACGAA pLKO.1 735 CDS 100% 3.000 2.100 N Akr7a5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025337.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11281 pDONR223 100% 79.1% 81.7% None (many diffs) n/a
2 ccsbBroad304_11281 pLX_304 0% 79.1% 81.7% V5 (many diffs) n/a
3 TRCN0000468610 GGACTAGCCACGGGGCCGGGCTGA pLX_317 39% 79.1% 81.7% V5 (many diffs) n/a
4 ccsbBroadEn_07822 pDONR223 100% 77.9% 77.1% None (many diffs) n/a
5 ccsbBroad304_07822 pLX_304 0% 77.9% 77.1% V5 (many diffs) n/a
6 TRCN0000469339 ACCTGAGCTCTACTCTGGTAGCAA pLX_317 41.9% 77.9% 77.1% V5 (many diffs) n/a
7 ccsbBroadEn_07823 pDONR223 100% 77.7% 76.2% None (many diffs) n/a
8 ccsbBroad304_07823 pLX_304 0% 77.7% 76.2% V5 (many diffs) n/a
9 TRCN0000465271 GACACAACAGCATGCGGAGCAAAC pLX_317 19.9% 77.7% 76.2% V5 (many diffs) n/a
10 ccsbBroadEn_13444 pDONR223 100% 36.6% 25.4% None (many diffs) n/a
11 ccsbBroad304_13444 pLX_304 0% 36.6% 25.4% V5 (many diffs) n/a
12 TRCN0000478142 GAGAGTCATAAAAGACCTGAGCGT pLX_317 70.7% 36.6% 25.4% V5 (many diffs) n/a
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