Transcript: Human NM_001320979.1

Homo sapiens aldo-keto reductase family 7 member A2 (AKR7A2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
AKR7A2 (8574)
Length:
2514
CDS:
23..997

Additional Resources:

NCBI RefSeq record:
NM_001320979.1
NBCI Gene record:
AKR7A2 (8574)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001320979.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232711 GTCTAGATCCATGCATTATTT pLKO_005 1102 3UTR 100% 15.000 21.000 N AKR7A2 n/a
2 TRCN0000038986 TGAGACCTACAGGAATCGCTT pLKO.1 688 CDS 100% 2.640 1.848 N AKR7A2 n/a
3 TRCN0000038984 CGAACTGGACACGGCCTTCAT pLKO.1 229 CDS 100% 1.650 1.155 N AKR7A2 n/a
4 TRCN0000232710 GACCTACAGGAATCGCTTCTG pLKO_005 691 CDS 100% 4.050 2.430 N AKR7A2 n/a
5 TRCN0000038988 GAGCAGAACTTGGCAGCAACA pLKO.1 887 CDS 100% 4.050 2.430 N AKR7A2 n/a
6 TRCN0000232707 GCTGGAGACGTCATTGAAGAG pLKO_005 388 CDS 100% 4.050 2.430 N AKR7A2 n/a
7 TRCN0000232708 TGGACCTCTTCTACCTACACG pLKO_005 426 CDS 100% 2.640 1.584 N AKR7A2 n/a
8 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 2327 3UTR 100% 4.950 2.475 Y ORAI2 n/a
9 TRCN0000038993 CAAGTACAAGTATGAGGACAA pLKO.1 622 CDS 100% 4.050 2.025 Y AKR7A3 n/a
10 TRCN0000038985 CCTTTAATCAAGCCTGGCATT pLKO.1 942 CDS 100% 4.050 2.025 Y AKR7A2 n/a
11 TRCN0000180418 GATCACTTGAGCTCAGGAGTT pLKO.1 2288 3UTR 100% 4.050 2.025 Y ERN2 n/a
12 TRCN0000046415 GCCTTTAATCAAGCCTGGCAT pLKO.1 941 CDS 100% 2.640 1.320 Y AKR7L n/a
13 TRCN0000046416 CGGTGGATGTACCACCACTCA pLKO.1 806 CDS 100% 0.088 0.044 Y AKR7L n/a
14 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2324 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001320979.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11281 pDONR223 100% 82.1% 82.1% None 1_87del;484_485ins105 n/a
2 ccsbBroad304_11281 pLX_304 0% 82.1% 82.1% V5 1_87del;484_485ins105 n/a
3 TRCN0000468610 GGACTAGCCACGGGGCCGGGCTGA pLX_317 39% 82.1% 82.1% V5 1_87del;484_485ins105 n/a
4 ccsbBroadEn_07822 pDONR223 100% 74.1% 71.8% None (many diffs) n/a
5 ccsbBroad304_07822 pLX_304 0% 74.1% 71.8% V5 (many diffs) n/a
6 TRCN0000469339 ACCTGAGCTCTACTCTGGTAGCAA pLX_317 41.9% 74.1% 71.8% V5 (many diffs) n/a
7 ccsbBroadEn_07823 pDONR223 100% 74% 71.3% None (many diffs) n/a
8 ccsbBroad304_07823 pLX_304 0% 74% 71.3% V5 (many diffs) n/a
9 TRCN0000465271 GACACAACAGCATGCGGAGCAAAC pLX_317 19.9% 74% 71.3% V5 (many diffs) n/a
10 ccsbBroadEn_13444 pDONR223 100% 40.8% 30.5% None (many diffs) n/a
11 ccsbBroad304_13444 pLX_304 0% 40.8% 30.5% V5 (many diffs) n/a
12 TRCN0000478142 GAGAGTCATAAAAGACCTGAGCGT pLX_317 70.7% 40.8% 30.5% V5 (many diffs) n/a
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