Construct: ORF ccsbBroad304_07972

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006743.1_s304c1, BRDN0000409763
Derived from:
ccsbBroadEn_07972
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHMP2B (25978)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07972
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25978 CHMP2B charged multivesicular body... NM_014043.4 99.8% 100% 312T>C
2 human 25978 CHMP2B charged multivesicular body... XM_011533576.2 91.8% 88.6% (many diffs)
3 human 25978 CHMP2B charged multivesicular body... NM_001244644.2 80.4% 80.2% 1_1delAins124;189T>C
4 mouse 68942 Chmp2b charged multivesicular body... NM_026879.2 89.6% 99.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
705
ORF length:
639
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gtccctcttc aagaagaaaa ccgtggatga tgtaataaag gaacagaatc
121gagagttacg aggtacacag agggctataa tcagagatcg agcagcttta gagaaacaag
181aaaaacagct ggaattagaa attaagaaaa tggccaagat tggtaataag gaagcttgca
241aagttttagc caaacaactt gtgcatctac ggaaacagaa gacgagaact tttgctgtaa
301gttcaaaagt tacttctatg tctacacaaa caaaagtgat gaattcccaa atgaagatgg
361ctggagcaat gtctaccaca gcaaaaacaa tgcaggcagt taacaagaag atggatccac
421aaaagacatt acaaacaatg cagaatttcc agaaggaaaa catgaaaatg gaaatgactg
481aagaaatgat caatgataca cttgatgaca tctttgacgg ttctgatgac gaagaagaaa
541gccaggatat tgtgaatcaa gttcttgatg aaattggaat tgaaatttct ggaaagatgg
601ccaaagctcc atcagctgct cgaagcttac catctgcctc tacttcaaag gctacaatct
661cagatgaaga gattgaacgg caactcaagg ctttaggagt agattaccca actttcttgt
721acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
781gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)