Construct: ORF ccsbBroad304_08173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012973.1_s304c1, BRDN0000462269
- Derived from:
- ccsbBroadEn_08173
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBTB21 (49854)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08173
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | NM_001098402.2 | 99.9% | 99.9% | 2881T>C |
| 2 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | NM_001320731.2 | 99.9% | 99.9% | 2881T>C |
| 3 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | NM_020727.5 | 99.9% | 99.9% | 2881T>C |
| 4 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | XM_005261121.3 | 99.9% | 99.9% | 2881T>C |
| 5 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | XM_011529588.2 | 99.9% | 99.9% | 2881T>C |
| 6 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | XM_011529590.2 | 99.9% | 99.9% | 2881T>C |
| 7 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | XM_017028360.1 | 99.9% | 99.9% | 2881T>C |
| 8 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | XM_017028361.1 | 99.9% | 99.9% | 2881T>C |
| 9 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | NM_001098403.2 | 81.1% | 81% | 1602_1603ins603;2278T>C |
| 10 | human | 49854 | ZBTB21 | zinc finger and BTB domain ... | NM_001320729.2 | 81.1% | 81% | 1602_1603ins603;2278T>C |
| 11 | mouse | 114565 | Zbtb21 | zinc finger and BTB domain ... | NM_001081684.2 | 82.5% | 86.2% | (many diffs) |
| 12 | mouse | 114565 | Zbtb21 | zinc finger and BTB domain ... | XM_006522860.3 | 82.5% | 86.2% | (many diffs) |
| 13 | mouse | 114565 | Zbtb21 | zinc finger and BTB domain ... | NM_175428.4 | 80.4% | 84% | (many diffs) |
| 14 | mouse | 114565 | Zbtb21 | zinc finger and BTB domain ... | XM_006522857.3 | 80.4% | 84% | (many diffs) |
| 15 | mouse | 114565 | Zbtb21 | zinc finger and BTB domain ... | XM_006522858.3 | 80.4% | 84% | (many diffs) |
| 16 | mouse | 114565 | Zbtb21 | zinc finger and BTB domain ... | XM_017316853.1 | 80.4% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3267
- ORF length:
- 3198
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagggatta ctgcattaca tcaaccctgc acacgccatt tctctcctaa 121 gtgccctgaa tgaggagcgt ctcaaaggac agctgtgtga tgtgctgctg attgttggag 181 accaaaagtt ccgagctcat aaaaacgtct tggctgccag cagcgaatac tttcagagtt 241 tattcacaaa taaggaaaat gagtcacaaa ctgtatttca gcttgacttc tgtgagccag 301 atgcttttga taatgtttta aactacattt attcttcctc tctatttgtt gagaagagca 361 gccttgctgc tgtgcaagaa cttggctata gtcttgggat ttcctttctg actaacatcg 421 tttctaaaac acctcaagcc ccctttccaa cgtgtcctaa tagaaaaaaa gtgtttgtag 481 aagatgatga aaacagttct caaaagagaa gtgtcattgt ttgtcaaagt agaaacgaag 541 cgcaaggaaa aactgttagt caaaatcaac ccgatgtaag ccatacttcc cggccctctc 601 ctagcattgc agtcaaggcc aataccaata agccacatgt cccaaaacca atagaaccac 661 ttcataattt gtcattaact gaaaagagtt ggccgaaaga tagttctgtg gtatatgcaa 721 agtctcttga gcattctgga tctttggatg atcctaatag aatcagtttg gtgaaaagaa 781 atgcagtgtt gccttcaaag cctctgcaag acagagaagc tatggatgat aaaccaggtg 841 tgagtggtca gcttccaaaa ggaaaagctc tagagctggc tttgaagaga ccacggccac 901 ctgttttgtc tgtttgtagc tcatcagaga ctccctatct attaaaagaa actaacaaag 961 gaaatggtca aggtgaagat agaaacttgt tgtactattc aaagttaggc ttagtgatcc 1021 catccagtgg atctggttct ggaaaccaaa gcattgacag gagtggccca cttgttaaga 1081 gtctcctcag acggtcattg tcgatggata gccaggttcc tgtctattca ccttccatag 1141 atttgaaatc ttcccaggga tcatcttcgg tgtccagtga tgcaccaggg aatgtgttgt 1201 gtgctttatc tcagaagtca tctttaaaag attgtagtga aaaaacagcc ctagatgaca 1261 ggcctcaagt gctacaaccg catcgcctca ggtcctttag tgcttctcag tcaacagaca 1321 gggagggagc ttcccctgtg actgaggtgc gcataaagac tgagcccagc agcccgctgt 1381 cggacccctc ggacatcatc cgcgtcactg tgggagatgc ggcaacaaca gcagctgcct 1441 catcttcgtc ggtcacaaga gacctgtctc tgaaaacaga agatgaccaa aaagacatga 1501 gcagactccc agcaaaaagg aggttccaag cggaccgaag attgccgttt aagaagttaa 1561 aggtgaatga gcacgggtct cctgtgtcag aagataattt tgaggaaggc tcaagcccta 1621 ctctccttga tgcagatttt ccagattctg atttgaataa agacgaattt ggtgagttgg 1681 aggggacgag accaaacaaa aaatttaaat gcaaacattg ccttaagatc tttagatcaa 1741 cagcaggtct tcaccgtcat gttaacatgt accataaccc agaaaagccc tacgcttgtg 1801 acatctgtca caagaggttt cacaccaact tcaaagtgtg gacacactgt cagacccaac 1861 acggcatagt gaagaaccca tcaccagcct ctagttcaca tgctgttttg gatgaaaaat 1921 tccaaagaaa gctgattgac atagtgagag agagagaaat taagaaggcc ctgatcatta 1981 agttaaggcg cggcaagcct ggttttcagg gacagagtag ctcccaagca cagcaagtca 2041 tcaagaggaa cttgagatct cgagccaaag gagcttacat ttgtacttac tgcggaaaag 2101 cgtaccgctt tctctctcaa tttaagcagc atataaaaat gcatccagga gaaaaacccc 2161 ttggagtaaa taaagttgct aaaccaaaag agcatgctcc tcttgcaagt ccagtagaaa 2221 acaaggaggt ttaccagtgc cgcctctgta atgctaagct ctcttctctc ctagagcaag 2281 gaagccacga gcggctgtgc cggaacgcgg ccgtctgccc ttactgcagc ctcaggtttt 2341 tctcgcccga gctgaagcaa gaacacgaga gcaagtgtga gtataagaag ctgacctgcc 2401 tcgagtgcat gcgcaccttc aagtcctctt tcagcatctg gcggcaccag gttgaagtcc 2461 ataatcagaa caacatggca cccaccgaaa acttttcttt gcccgttttg gaccacaatg 2521 gtgatgtgac tggttcttca aggccccaat cccagcctga gcccaacaaa gtaaaccaca 2581 tcgtcaccac aaaagacgac aacgtgttca gtgattcttc agaacaagtt aacttcgact 2641 cggaagattc ctcttgtctt cccgaagacc ttagtctttc caagcaactg aaaatccaag 2701 tcaaagagga gcctgtggag gaggctgaag aagaggcacc cgaggccagc acagccccca 2761 aagaagcggg tcctagcaaa gaagccagcc tgtggccctg cgagaagtgt gggaagatgt 2821 tcacggtgca taagcagctg gagcgtcacc aggagcttct gtgctctgtg aaaccattta 2881 tttgtcacgt gtgcaacaaa gcttttcgca ctaattttcg actctggagt cacttccaat 2941 cgcacatgcc tcaggcttca gaggaatcgg cacataagga atctgaggtg tgccctgttc 3001 ccacaaactc tccctctcca ccacctctgc caccgccacc accactgccc aagatccagc 3061 ctctggagcc tgacagcccc acaggcctgt ccgaaaaccc aactccagcc acagaaaaac 3121 tgtttgtgcc ccaagaatca gacacccttt tttaccatgc cccacccctt tcagcaatca 3181 catttaaaag acagtttatg tgtaaacttt gccacaggac attcaagact gcatttagtc 3241 tttggagtca cgaacaaaca cacaatttgc caactttctt gtacaaagtg gttggtaagc 3301 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3361 ggcgcgttaa gtcgacaatc aacctctgga tta