Construct: ORF ccsbBroad304_08216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004714.1_s304c1, BRDN0000406718
- Derived from:
- ccsbBroadEn_08216
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRNAD1 (51093)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08216
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | NM_015997.4 | 99.9% | 99.7% | 1198G>C |
| 2 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_005245234.5 | 91.4% | 92.2% | 1198G>C;1306_1318del;1329_1330ins109 |
| 3 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_011509600.1 | 86% | 85.8% | 0_1ins198;1000G>C |
| 4 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_017001404.1 | 86% | 85.8% | 0_1ins198;1000G>C |
| 5 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_005245235.5 | 85.1% | 85% | 111_112ins210;988G>C |
| 6 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XR_426777.4 | 72.3% | (many diffs) | |
| 7 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XR_426778.4 | 70% | (many diffs) | |
| 8 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_005245236.5 | 68.9% | 68.8% | 680_681ins441;757G>C |
| 9 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_005245237.5 | 64.8% | 46.8% | (many diffs) |
| 10 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_017001405.2 | 64.1% | 64% | 0_1ins510;688G>C |
| 11 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_017001406.2 | 64.1% | 64% | 0_1ins510;688G>C |
| 12 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | XM_024447421.1 | 60.7% | 61.2% | (many diffs) |
| 13 | human | 51093 | RRNAD1 | ribosomal RNA adenine dimet... | NM_001142560.2 | 58.2% | 46.8% | 636_637ins485;713G>C;831_832ins109 |
| 14 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | NM_153562.4 | 86.2% | 86.5% | (many diffs) |
| 15 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | XM_006501339.3 | 82.2% | 82.3% | (many diffs) |
| 16 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | XM_006501340.3 | 74.8% | 72% | (many diffs) |
| 17 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | XR_375513.3 | 57.2% | (many diffs) | |
| 18 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | XR_375514.3 | 55.9% | (many diffs) | |
| 19 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | XM_011240085.2 | 54.6% | 54.9% | (many diffs) |
| 20 | mouse | 229503 | Rrnad1 | ribosomal RNA adenine dimet... | XM_011240086.2 | 54.6% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc gggcatctcc gcccgaggcc tctctcatga ggggaggaag cagctagctg 121 ttaacctcac ccgtgtcctg gcactctacc gttccatctt ggatgcctac atcatcgaat 181 ttttcacaga caacctatgg gacacactcc cttgctcatg gcaggaagca ttggatggac 241 tgaaaccacc acagctggcc acaatgctgc tggggatgcc tggggaaggg gaggtcgtca 301 ggtacaggtc agtgtggcca ctcaccctgc tggccctgaa gtccacggcg tgtgccctgg 361 cctttacccg gatgcctggc tttcagaccc cctcagaatt cctggagaac cccagccaga 421 gctcccgact aacagctcca ttccggaaac atgtcaggcc caagaagcag catgagatcc 481 ggaggctggg agagttggtg aagaagctga gtgatttcac aggctgcacc caggttgtag 541 acgtgggctc aggccagggc catctctccc gcttcatggc tcttggcctg gggttgatgg 601 tgaagagcat cgaaggggat cagagactgg tggagagagc ccagcgcctg gaccaggagc 661 ttctgcaggc tctggagaaa gaggagaaga ggaacccgca ggtggtccaa accagccctc 721 gtcactcccc acaccacgtg gttaggtggg tagaccccac agccctgtgt gaggagcttc 781 tgcttccact ggagaacccg tgtcagggca gggcccgctt gctgctcaca ggcctccacg 841 cctgtgggga tctgagtgtt gccttgctga gacacttctc ctgctgtcct gaggtggtgg 901 ccctggcctc agtgggctgc tgctacatga agctgagtga ccctggcggc tacccactga 961 gtcagtgggt ggctgggctg cctggctatg aactgcccta ccggcttcgg gagggggcct 1021 gccatgccct ggaggaatat gctgagcggc tacagaaagc tggccctggc cttcgaactc 1081 actgctaccg tgcagcactg gagacagtca tccgacgggc ccggcccgag ctccgtcggc 1141 caggcgtgca gggtatcccc agggtccacg agctcaagat tgaagaatat gtgcagcggg 1201 ggctacagcg agtggggcta gatccccagc tgccactgaa tctggctgcc cttcaggccc 1261 acctggccca ggagaaccgt gtggtggcct tcttcagcct ggctctactg cttgccccac 1321 tggtggagac gcttattcta ctggaccggc tgctgtacct tcaggaacag ggtttccatg 1381 ctgagctcct gcccatcttc agtcctgaac tctctcccag aaacctggtt ctggtggcca 1441 ccaagatgcc cctgggtcag gctctttctg ttctggagac tgaagacagc tgcccaactt 1501 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1561 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta