Construct: ORF ccsbBroad304_08258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002504.1_s304c1, BRDN0000462259
- Derived from:
- ccsbBroadEn_08258
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF20 (51230)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08258
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51230 | PHF20 | PHD finger protein 20 | NM_016436.5 | 99.9% | 99.9% | 1813G>A |
| 2 | human | 51230 | PHF20 | PHD finger protein 20 | XM_017027868.2 | 94.5% | 94.4% | 255_256ins165;1648G>A |
| 3 | human | 51230 | PHF20 | PHD finger protein 20 | XM_017027869.2 | 88.5% | 85.5% | 0_1ins268;71_72ins80;1465G>A |
| 4 | human | 51230 | PHF20 | PHD finger protein 20 | XM_017027870.2 | 88.5% | 85.5% | 0_1ins268;71_72ins80;1465G>A |
| 5 | human | 51230 | PHF20 | PHD finger protein 20 | XM_024451889.1 | 88.5% | 85.5% | 0_1ins268;71_72ins80;1465G>A |
| 6 | human | 51230 | PHF20 | PHD finger protein 20 | XM_005260421.3 | 60.1% | 60% | 0_1ins1209;604G>A |
| 7 | human | 51230 | PHF20 | PHD finger protein 20 | XM_011528843.2 | 60.1% | 60% | 0_1ins1209;604G>A |
| 8 | human | 51230 | PHF20 | PHD finger protein 20 | XM_011528844.2 | 60.1% | 60% | 0_1ins1209;604G>A |
| 9 | human | 51230 | PHF20 | PHD finger protein 20 | XM_017027871.2 | 60.1% | 60% | 0_1ins1209;604G>A |
| 10 | human | 51230 | PHF20 | PHD finger protein 20 | XM_024451890.1 | 53.3% | 53.2% | 0_1ins1416;397G>A |
| 11 | mouse | 228829 | Phf20 | PHD finger protein 20 | NM_172674.2 | 87.1% | 88.5% | (many diffs) |
| 12 | mouse | 228829 | Phf20 | PHD finger protein 20 | XM_017317827.1 | 82% | 83.3% | (many diffs) |
| 13 | mouse | 228829 | Phf20 | PHD finger protein 20 | XM_006499338.3 | 73.4% | 74.3% | (many diffs) |
| 14 | mouse | 228829 | Phf20 | PHD finger protein 20 | XM_006499339.1 | 52.1% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3105
- ORF length:
- 3036
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaaagcat ccacctaaca gacgaggaat cagctttgaa gtgggagccc 121 agttggaagc ccgggaccgt ttaaaaaact ggtatccagc tcacatagaa gacattgact 181 acgaggaagg aaaagtactc atccatttca agcgttggaa ccatcgttat gatgagtggt 241 tctgctggga cagtccttat ttacgccctt tagagaaaat acagctgagg aaagagggct 301 tgcatgaaga ggatggatct tctgaatttc aaataaatga gcaggtcctt gcttgctggt 361 ctgattgtcg tttttacccg gccaaagtca ctgctgttaa caaggatggt acttacactg 421 tgaaatttta tgatggagta gttcagactg tcaaacatat tcatgtcaaa gctttttcca 481 aagatcagaa tattgtgggt aatgctaggc ctaaagaaac agatcacaaa agtctttcat 541 catctcctga taaacgagag aagtttaaag aacagagaaa agcaacagtg aatgtgaaga 601 aagacaaaga agataaaccc ttaaagacag aaaagcgacc caagcagcct gataaagaag 661 gaaagttaat ctgttctgaa aaggggaaag tgtcagagaa aagtcttccc aagaacgaga 721 aggaagacaa ggaaaacatt tccgaaaatg acagagagta ttctggagat gcccaagtgg 781 ataagaaacc tgaaaatgac attgtgaaga gtccacaaga aaacttgagg gaacccaaaa 841 gaaaacgagg cagaccccct tccatagctc ctactgctgt ggattcaaac tctcaaactt 901 tgcaaccaat aacattggaa ctgagaagaa ggaaaatatc aaaaggatgt gaagtcccat 961 taaaacgtcc tcggcttgac aaaaattcat cccaggaaaa gtcaaaaaac tactcggaaa 1021 acactgacaa agacttatcg aggagacgtt cctccaggct gtccactaat gggacccatg 1081 agatcctaga tcctgacttg gttgtatcag atttggttga tacggatcct ttgcaagaca 1141 cgttgtctag taccaaggaa tctgaagaag gtcagttgaa gtctgctttg gaagctggcc 1201 aggtctcatc tgcactgact tgccactcct ttggggatgg atccggggct gcaggcttgg 1261 agttgaactg cccatcaatg ggagaaaaca cgatgaaaac agaaccgact tctccccttg 1321 tggaattaca agagatttcg actgtggaag taacaaatac ttttaagaaa acagatgatt 1381 ttgggtcatc taatgcacca gctgtcgacc tagaccataa gtttagatgc aaagttgtgg 1441 actgtttaaa atttttccgc aaagccaaac tgttgcacta tcacatgaag tatttccatg 1501 gaatggagaa gtcactggag ccagaagaga gcccgggaaa gaggcatgtc caaaccaggg 1561 gcccttcagc ttcagacaag cccagccagg agaccctgac caggaagcgg gtctctgcca 1621 gttccccaac tacaaaagac aaggaaaaga ataaagagaa gaaattcaag gagtttgtga 1681 gagtgaagcc aaagaagaaa aagaaaaaga aaaagaaaac caaacctgaa tgcccctgca 1741 gtgaggagat cagtgacacc tcccaggaac cttctccacc caaggcattt gctgttacca 1801 ggtgtgggtc ctcacacaag ccaggggtcc atatgagccc gcagcttcat ggcccagaat 1861 ctggacacca caaagggaaa atgaaagcat tggaggagga taatttgagt gagtcctctt 1921 ctgagagctt tctctggagt gatgatgagt atggccaaga tgtggatgtg accaccaacc 1981 cagatgagga acttgatggg gatgaccgct atgacttcga ggtggtccgc tgcatctgtg 2041 aggtccagga ggaaaatgac ttcatgattc agtgtgaaga gtgccagtgc tggcagcatg 2101 gggtctgcat gggattactg gaagaaaatg tgcccgagaa atacacctgt tatgtttgcc 2161 aagaccctcc aggtcagagg cctggcttca agtactggta tgacaaggag tggctgagca 2221 ggggacatat gcatggcctg gcatttctag aagagaacta ctcccatcag aatgccaaga 2281 agatcgtggc cacccaccag cttcttggtg atgtgcagag agtgattgag gttctgcatg 2341 gcctgcagct caagatgagc atcttgcaaa gccgggagca tcctgatctg ccgctgtggt 2401 gccagccttg gaaacagcac tcaggggagg ggagatctca tttcagaaac atccctgtca 2461 ctgacaccag gagcaaggag gaagctccaa gctatagaac tttgaacggg gcagtggaga 2521 agcccaggcc cctggccctg cccctgccgc gttctgtgga ggaatcctat atcaccagtg 2581 agcattgcta ccagaagccc cgcgcctatt accctgccgt ggagcagaag ctggtggtgg 2641 agacgagggg ctctgccctc gacgatgcgg tcaaccccct ccatgagaac ggcgatgatt 2701 ccctttcccc gcgcctgggc tggcctctag accaagacag gagcaagggg gacagtgacc 2761 ccaaacccgg ctccccaaag gtgaaggaat atgtctccaa aaaggcccta ccagaagaag 2821 cccctgctcg gaagctgctg gacagaggtg gagaggggct gctgagctcc cagcaccagt 2881 ggcagtttaa cctgctgacc catgtggaat ctcttcagga tgaagttacg cacaggatgg 2941 actccattga gaaggagttg gatgtgttgg agagctggct ggactacact ggggaactgg 3001 agccccctga gccgctggcc aggcttccgc agctcaagca ttgtatcaag cagctgctga 3061 tggacctggg caaggtgcag cagatcgccc tctgctgctc aacattgcca actttcttgt 3121 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 3181 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a