Transcript: Human XM_017027869.2

PREDICTED: Homo sapiens PHD finger protein 20 (PHF20), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PHF20 (51230)
Length:
5893
CDS:
458..3148

Additional Resources:

NCBI RefSeq record:
XM_017027869.2
NBCI Gene record:
PHF20 (51230)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027869.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000016313 CCCGAGAAATACACCTGTTAT pLKO.1 2174 CDS 100% 13.200 18.480 N PHF20 n/a
2 TRCN0000379949 AGCGTTGGAACCATCGTTATG pLKO_005 332 5UTR 100% 10.800 15.120 N PHF20 n/a
3 TRCN0000225873 GTGGGTAATGCTAGGCCTAAA pLKO_005 536 CDS 100% 10.800 15.120 N Phf20 n/a
4 TRCN0000382197 GTGGGTAATGCTAGGCCTAAA pLKO_005 536 CDS 100% 10.800 15.120 N PHF20 n/a
5 TRCN0000379882 AGCATTGGAGGAGGATAATTT pLKO_005 1927 CDS 100% 15.000 10.500 N PHF20 n/a
6 TRCN0000016314 CCTGATAAAGAAGGAAAGTTA pLKO.1 689 CDS 100% 5.625 3.938 N PHF20 n/a
7 TRCN0000016316 GCATGGGATTACTGGAAGAAA pLKO.1 2148 CDS 100% 5.625 3.938 N PHF20 n/a
8 TRCN0000016315 CCAGCTCACATAGAAGACATT pLKO.1 277 5UTR 100% 4.950 3.465 N PHF20 n/a
9 TRCN0000016317 CCTGACTTGGTTGTATCAGAT pLKO.1 1133 CDS 100% 4.950 3.465 N PHF20 n/a
10 TRCN0000380621 ATTGTGCCACTGATGATAAAC pLKO_005 3435 3UTR 100% 13.200 7.920 N PHF20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027869.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08258 pDONR223 100% 88.5% 85.5% None 0_1ins268;71_72ins80;1465G>A n/a
2 ccsbBroad304_08258 pLX_304 0% 88.5% 85.5% V5 0_1ins268;71_72ins80;1465G>A n/a
3 TRCN0000481434 CCCTCCTTGTTAAAGCGGCATCAG pLX_317 14.2% 88.5% 85.5% V5 0_1ins268;71_72ins80;1465G>A n/a
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