Construct: ORF ccsbBroad304_08454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012942.1_s304c1, BRDN0000409295
- Derived from:
- ccsbBroadEn_08454
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BANK1 (55024)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08454
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55024 | BANK1 | B cell scaffold protein wit... | NM_001083907.3 | 99.9% | 99.8% | 1230G>A;1858T>C |
| 2 | human | 55024 | BANK1 | B cell scaffold protein wit... | XM_017008337.2 | 99.9% | 99.8% | 1230G>A;1858T>C |
| 3 | human | 55024 | BANK1 | B cell scaffold protein wit... | NM_017935.5 | 96% | 96% | 1_90del;1320G>A;1948T>C |
| 4 | human | 55024 | BANK1 | B cell scaffold protein wit... | NM_001127507.2 | 84.5% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2331
- ORF length:
- 2265
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat atatgaagaa gatgctgagg aatgggctct gtacttgaca gaagtatttt 121 tacatgttgt gaaaagggaa gccatcctgt tatatcgctt ggagaatttc tcttttcggc 181 atttggagtt gctgaactta acgtcttaca aatgtaaact tttgatatta tcaaatagcc 241 tgcttagaga cctaactcca aagaaatgtc agtttctgga aaagatactt cattcaccaa 301 aaagtgtagt tactttgctt tgtggagtga agagttcaga tcagctctat gaattactaa 361 atatctctca aagcagatgg gagatctcaa ctgaacagga acctgaagac tacatctctg 421 taatccagag tatcatattc aaagattctg aagactactt tgaggtcaac attccaacag 481 acctacgagc aaaacattct ggggaaataa gtgagagaaa ggaaattgaa gaactatcag 541 aagcttcaag aaacaccata ccactagcag tggtgcttcc cactgaaatt ccatgtgaga 601 atcctggtga aatattcata attttgagag atgaagtaat tggtgatact gtagaggttg 661 aatttacatc aagtaataag cgcattagaa cacggccagc cctttggaat aagaaagtct 721 ggtgcatgaa agctttagag tttcctgctg gttcagtcca tgtcaatgtc tactgtgatg 781 gaatcgttaa agctacaacc aaaattaagt actacccaac agcaaaggca aaggaatgcc 841 tattcagaat ggcagattca ggagagagtt tgtgccagaa tagcattgaa gaacttgatg 901 gtgtccttac atccatattc aaacatgaga taccatatta tgagttccag tctcttcaaa 961 ctgaaatttg ttctcaaaac aaatatactc atttcaaaga acttccaact cttctccact 1021 gtgcagcaaa atttggctta aagaacctgg ctattcattt gcttcaatgt tcaggagcaa 1081 cctgggcatc taagatgaaa aatatggagg gttcagaccc cgcacatatt gctgaaaggc 1141 atggtcacaa agaactcaag aaaatcttcg aagacttttc aatccaagaa attgacataa 1201 ataatgagca agaaaatgat tatgaagagg atattgcctc attttccaca tatattcctt 1261 ccacacagaa cccagcattt catcatgaaa gcagaaagac atacgggcag agtgcagatg 1321 gagctgaggc aaatgaaatg gaaggggaag gaaaacagaa tggatcaggc atggagacca 1381 aacacagccc actagaggtt ggcagtgaga gttctgaaga ccagtatgat gacttgtatg 1441 tgttcattcc tggtgctgat ccagaaaata attcacaaga gccactcatg agcagcagac 1501 ctcctctccc cccgccgcga cctgtagcta atgccttcca actggaaaga cctcacttca 1561 ccttaccagg gacaatggtg gaaggccaaa tggaaagaag tcaaaactgg ggtcatcctg 1621 gtgttagaca agaaacagga gatgaaccca aaggagaaaa agagaagaaa gaagaggaaa 1681 aagagcagga ggaggaagaa gacccatata cttttgctga gattgatgac agtgaatatg 1741 acatgatatt ggccaatctg agtataaaga aaaaaactgg gagtcggtct ttcattataa 1801 atagacctcc tgcccccaca ccccgaccca caagtatacc tccaaaagag gaaactacac 1861 cttacatagc tcaagtgttt caacaaaaga cagccagaag acaatctgat gatgacaagt 1921 tccgtggtct tcctaagaaa caagacagag ctcggataga gagtccagcc ttttctactc 1981 tcaggggctg tctaactgat ggtcaggaag aactcatcct cctgcaggag aaagtaaaga 2041 atgggaaaat gtctatggat gaagctctgg agaaatttaa acactggcag atgggaaaaa 2101 gtggcctgga aatgattcag caggagaaat tacgacaact acgagactgc attattggga 2161 aaaggccaga agaagaaaat gtctataata aactcaccat tgtgcaccat ccaggtggta 2221 aggaaactgc ccacaatgaa aataagtttt ataatgtaca cttcagcaat aagcttcctg 2281 ctcgacccca agttgaaaag gaatttggtt tctgttgcaa gaaagatcat tacccaactt 2341 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2401 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta