Construct: ORF ccsbBroad304_08532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014417.1_s304c1, BRDN0000400917
- Derived from:
- ccsbBroadEn_08532
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIRPG (55423)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08532
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55423 | SIRPG | signal regulatory protein g... | NM_018556.4 | 99.8% | 100% | 1095C>T;1140C>T |
| 2 | human | 55423 | SIRPG | signal regulatory protein g... | XM_011529286.2 | 91.3% | 91.4% | 0_1ins99;996C>T;1041C>T |
| 3 | human | 10326 | SIRPB1 | signal regulatory protein b... | NM_001135844.4 | 85.4% | 78.3% | (many diffs) |
| 4 | human | 10326 | SIRPB1 | signal regulatory protein b... | NM_001329157.2 | 85.4% | 78.3% | (many diffs) |
| 5 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_005260641.3 | 84.7% | 76% | (many diffs) |
| 6 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_011529134.2 | 84.6% | 73.8% | (many diffs) |
| 7 | human | 10326 | SIRPB1 | signal regulatory protein b... | NM_006065.4 | 84.2% | 75.3% | (many diffs) |
| 8 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027577.1 | 84.2% | 75.3% | (many diffs) |
| 9 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027578.1 | 83.8% | 71.6% | (many diffs) |
| 10 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027579.1 | 83.8% | 71.6% | (many diffs) |
| 11 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027580.1 | 83.8% | 71.6% | (many diffs) |
| 12 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027583.1 | 81.8% | 73.4% | (many diffs) |
| 13 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_017027584.1 | 81.8% | 73.4% | (many diffs) |
| 14 | human | 55423 | SIRPG | signal regulatory protein g... | XM_005260749.4 | 72.4% | 72.6% | 429_430ins318;777C>T;822C>T |
| 15 | human | 55423 | SIRPG | signal regulatory protein g... | NM_001039508.1 | 71.1% | 71.3% | 747_748ins333;762C>T;807C>T |
| 16 | human | 10326 | SIRPB1 | signal regulatory protein b... | XR_001754121.1 | 70.2% | (many diffs) | |
| 17 | human | 55423 | SIRPG | signal regulatory protein g... | XM_011529287.2 | 66.9% | 65.1% | (many diffs) |
| 18 | human | 140885 | SIRPA | signal regulatory protein a... | NM_001040022.1 | 66% | 55.5% | (many diffs) |
| 19 | human | 140885 | SIRPA | signal regulatory protein a... | NM_001040023.2 | 66% | 55.5% | (many diffs) |
| 20 | human | 140885 | SIRPA | signal regulatory protein a... | NM_080792.3 | 66% | 55.5% | (many diffs) |
| 21 | human | 140885 | SIRPA | signal regulatory protein a... | NM_001330728.1 | 65.5% | 55.1% | (many diffs) |
| 22 | human | 140885 | SIRPA | signal regulatory protein a... | XM_005260670.3 | 65.5% | 55.1% | (many diffs) |
| 23 | human | 140885 | SIRPA | signal regulatory protein a... | XM_024451836.1 | 65.5% | 55.1% | (many diffs) |
| 24 | human | 10326 | SIRPB1 | signal regulatory protein b... | XM_005260643.3 | 64.6% | 55.9% | (many diffs) |
| 25 | human | 140885 | SIRPA | signal regulatory protein a... | XM_011529173.2 | 63.6% | 54.6% | (many diffs) |
| 26 | human | 55423 | SIRPG | signal regulatory protein g... | NM_080816.3 | 41.6% | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgtcccagcc tcctggcccc atcctcctgg tcctttcctg cttctgactc 121 tactgctggg acttacagaa gtggcaggtg aggaggagct acagatgatt cagcctgaga 181 agctcctgtt ggtcacagtt ggaaagacag ccactctgca ctgcactgtg acctccctgc 241 ttcccgtggg acccgtcctg tggttcagag gagttggacc aggccgggaa ttaatctaca 301 atcaaaaaga aggccacttc cccagggtaa caacagtttc agacctcaca aagagaaaca 361 acatggactt ttccatccgc atcagtagca tcaccccagc agatgtcggc acatactact 421 gtgtgaagtt tcgaaaaggg agccctgaga acgtggagtt taagtctgga ccaggcactg 481 agatggcttt gggtgccaaa ccctctgccc ccgtggtatt gggccctgcg gcgaggacca 541 cacctgagca tacagtgagt ttcacctgtg agtcccatgg cttctctccc agagacatca 601 ccctgaaatg gttcaaaaat gggaatgagc tctcagactt ccagaccaac gtggacccca 661 caggacagag tgtggcctac agcatccgca gcacagccag ggtggtactg gacccctggg 721 acgttcgctc tcaggtcatc tgcgaggtgg cccatgtcac cttgcagggg gaccctcttc 781 gtgggactgc caacttgtct gaggccatcc gagttccacc caccttggag gttactcaac 841 agcccatgag ggtggggaac caggtaaacg tcacctgcca ggtgaggaag ttctaccccc 901 agagcctaca gctgacctgg tcggagaatg gaaacgtgtg ccagagagaa acagcctcga 961 cccttacaga gaacaaggat ggtacctaca actggacaag ctggttcctg gtgaacatat 1021 ctgaccaaag ggatgatgtg gtcctcacct gccaggtgaa gcatgatggg cagctggcgg 1081 tcagcaaacg ccttgcccta gaggtcacag tccaccagaa ggaccagagc tcagatgcta 1141 cccctggccc ggcatcatct cttactgcgc tgctcctcat agctgtcctc ctgggcccca 1201 tctatgtccc ctggaagcag aagacctgcc caactttctt gtacaaagtg gttggtaagc 1261 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1321 ggcgcgttaa gtcgacaatc aacctctgga tta