Construct: ORF ccsbBroad304_08557
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018077.1_s304c1, BRDN0000410658
- Derived from:
- ccsbBroadEn_08557
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMBRA1 (55626)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08557
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001367471.1 | 99.9% | 99.8% | 102A>G;3043C>G;3532C>G |
| 2 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_017749.3 | 99.9% | 99.8% | 102A>G;3043C>G;3532C>G |
| 3 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001267783.2 | 97.5% | 97.4% | (many diffs) |
| 4 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001367468.1 | 92.9% | 92.9% | (many diffs) |
| 5 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | XM_005253009.4 | 92.9% | 92.9% | (many diffs) |
| 6 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | XM_006718259.2 | 92.9% | 92.9% | (many diffs) |
| 7 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | XM_024448605.1 | 92.9% | 92.9% | (many diffs) |
| 8 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001267782.2 | 92.7% | 92.6% | (many diffs) |
| 9 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001367469.1 | 92.5% | 92.4% | (many diffs) |
| 10 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | XM_005253011.4 | 90.7% | 90.6% | (many diffs) |
| 11 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001300731.2 | 88.3% | 88.2% | (many diffs) |
| 12 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NM_001367470.1 | 85.1% | 85% | 0_1ins537;2506C>G;2995C>G |
| 13 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | XM_024448606.1 | 76.4% | 74.7% | (many diffs) |
| 14 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | XR_002957153.1 | 69.1% | (many diffs) | |
| 15 | human | 55626 | AMBRA1 | autophagy and beclin 1 regu... | NR_160027.1 | 62.5% | (many diffs) | |
| 16 | mouse | 228361 | Ambra1 | autophagy/beclin 1 regulator 1 | NM_001080754.1 | 91.5% | 95.6% | (many diffs) |
| 17 | mouse | 228361 | Ambra1 | autophagy/beclin 1 regulator 1 | XM_006499253.1 | 89.2% | 93.2% | (many diffs) |
| 18 | mouse | 228361 | Ambra1 | autophagy/beclin 1 regulator 1 | NM_172669.3 | 85.1% | 88.9% | (many diffs) |
| 19 | mouse | 228361 | Ambra1 | autophagy/beclin 1 regulator 1 | XM_006499252.1 | 82.9% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3690
- ORF length:
- 3624
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggttgtccca gaaaagaatg ctgtccggat actctggggg cgagaacggg 121 gtgctcgggc catgggagct cagcggcttc tgcaggagct ggtagaggat aaaacccggt 181 ggatgaaatg ggagggcaag agagtagaac tgccggatag tccacgctct accttcttat 241 tggccttcag cccagacagg actctcttag cctccaccca tgtgaaccat aatatctata 301 ttacggaggt gaagactggc aagtgtgttc attccctgat tggacaccgc cgtactccat 361 ggtgtgtcac ttttcatccc accatctcag gccttattgc ttctggctgc ctagatgggg 421 aggttaggat ttgggattta cacggtggca gtgaaagctg gttcacagat agcaacaatg 481 ccattgcctc cctggctttc caccctacgg ctcagctcct gctgattgcc actgccaatg 541 agatccactt ctgggactgg agtcgacggg aaccctttgc tgtggtgaag acagctagtg 601 agatggaacg ggtccgtctg gtgagatttg atccacttgg acactactta ctcacagcaa 661 ttgttaaccc ctctaatcaa cagggtgatg acgaaccaga gatccccata gatggaacag 721 aattatccca ctaccgtcag cgtgccctcc tgcaatcaca gccagttcgc cggacgcctc 781 tcctccacaa tttcctgcac atgctgtcct cccgctcttc tggcatccag accgagccct 841 tccatccccc ggagcaggcc tcgtcaacgc agcaggacca gggcctcctg aaccggccgt 901 ctgccttcag tacagtccag agcagcactg ccggcaacac gctccgcaac ctcagtctgg 961 gtcctacccg ccgctctttg ggagggcctc tgtctagcca cccttctagg tatcaccgag 1021 aaatagctcc tgggttgaca ggatctgagt ggacccggac agtactcagt ctgaactccc 1081 gctctgaggc ggaatccatg cccccgccca gaaccagtgc ctcttcggtg agtttgctgt 1141 ctgtgctgag acagcaggaa ggtggctctc aggcatctgt gtacacttca gccacagaag 1201 ggaggggttt tccggcatca gggttggcaa ctgagtcaga tggagggaat ggctccagcc 1261 aaaacaactc gggcagcatt cgccatgagc ttcagtgtga cctgagacgc ttctttctgg 1321 agtatgaccg gcttcaggag ctggatcaga gcctgagtgg ggaagctccc cagacccaac 1381 aggcccagga aatgctcaac aataacattg aatctgagag gccaggccct tcccaccagc 1441 ccaccccaca cagcagtgag aacaactcca acctgtcccg tggccacctg aatcgctgtc 1501 gtgcttgcca caatctcctg accttcaaca acgataccct gcgctgggaa agaaccacac 1561 ctaactactc ctctggcgag gctagttcct cttggcaggt ccccagctcc tttgagagtg 1621 tgccatcaag tggcagccag ttgccacctc tcgagcggac tgagggccaa acgcccagct 1681 ccagcaggct ggagttgagc agctctgcta gtccgcagga ggagaggact gtgggggtgg 1741 cctttaacca ggagacaggc cactgggaaa gaatttacac ccagtccagc agatctggaa 1801 ctgtgtcaca ggaggcctta catcaggata tgcctgagga gagctctgag gaggattcac 1861 tcaggaggag gctgctggaa tcttccctca tttcattatc ccgttatgat ggagcaggat 1921 ccagagagca cccaatttac ccagacccag cgagattatc tcctgctgca tactacgccc 1981 agaggatgat ccagtatctc tcacggagag acagtattcg ccagcgctcc atgcgctacc 2041 aacagaaccg tctccgttct tccacctcct cctcttcctc agacaaccag ggtccatcag 2101 tagagggaac cgacttggaa tttgaggact ttgaggacaa tggtgacaga tccaggcacc 2161 gagctccacg caatgcccgg atgtctgcac cttcgcttgg acgctttgtc ccaaggcgtt 2221 tcttgctgcc tgagtacttg ccttatgctg ggatttttca tgaacgtgga cagcctggct 2281 tggctactca ctcttctgtt aacagggtcc tggcaggggc agtgatcggt gatggacagt 2341 ctgctgtggc cagtaacatt gccaatacta cctaccggct ccagtggtgg gacttcacta 2401 agtttgacct ccctgaaatc agtaatgctt ccgtgaatgt gctggtgcag aactgcaaga 2461 tctacaatga tgccagctgt gacatttctg cagatggcca gctcctggca gctttcatcc 2521 ccagcagcca gaggggcttt cctgatgaag gcatcctggc agtgtactcc ctggcccccc 2581 ataacctggg cgaaatgctc tacaccaagc gatttggtcc caatgccatt tcggtgagcc 2641 tgtccccaat gggcagatat gtaatggtgg gcttggcctc acgaaggatc ctgctgcacc 2701 cctccacaga gcacatggtg gcccaggtct tcaggctgca acaggcccat ggtggagaga 2761 cctccatgag gagagttttc aacgtccttt atcccatgcc tgccgaccag cggagacatg 2821 tcagtatcaa ctctgcccgt tggctgcctg agccagggct tggcttggcc tatggtacta 2881 acaaaggaga cctggtgatc tgccgaccag aggccttaaa ctctggtgtt gagtactact 2941 gggaccagct gaacgagacg gtcttcactg tccattccaa cagcaggagc agcgagcggc 3001 ctggaaccag cagagccaca tggaggacag acagagacat ggggctgatg aatgccattg 3061 ggcttcagcc ccggaaccct gccacctcag tgacatctca gggcaccgag actctggccc 3121 ttcagctgca gaatgccgaa acacagactg agagggaggt gccggagcca gggacagccg 3181 cctcaggtcc tggtgaaggt gagggttcag agtatggtgc cagtggagaa gatgcgctca 3241 gcaggatcca gaggctgatg gcggagggcg gcatgacagc cgtggtgcag cgggagcaga 3301 gcaccaccat ggcctccatg ggcggcttcg gcaacaacat catcgtcagc caccgcattc 3361 accgcagctc tcagacgggc actgaacctg gtgccgccca cacctcctca ccccagccct 3421 ccacctctcg gggactgctc ccagaggccg ggcaactggc agagcgaggc ctaagccccc 3481 ggacagcttc ctgggaccag cctggtaccc ctgggcggga gccaacccag ccaaccctgc 3541 cctcttcctc ccctgtcccc attcctgttt cccttcccag cgctgaggga ccaaccgtcc 3601 actgcgagtt gaccaataac aaccaccttc tggatggtgg cagcagcagg ggggacgctg 3661 caggccctag gggagaacca cggaacaggt acccaacttt cttgtacaaa gtggttggta 3721 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 3781 ggtggcgcgt taagtcgaca atcaacctct ggatta