Construct: ORF ccsbBroad304_08599

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004168.1_s304c1, BRDN0000404503
Derived from:
ccsbBroadEn_08599
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DENND10P1 (55855)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08599
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 404636 DENND10 DENN domain containing 10 NM_207009.4 99.5% 98.8% (many diffs)
2 human 404636 DENND10 DENN domain containing 10 NM_001303111.2 94.4% 93.8% (many diffs)
3 human 404636 DENND10 DENN domain containing 10 XM_017016261.1 94.4% 93.8% (many diffs)
4 human 404636 DENND10 DENN domain containing 10 XM_017016262.2 94.4% 93.8% (many diffs)
5 human 404636 DENND10 DENN domain containing 10 XM_017016263.1 94.4% 93.8% (many diffs)
6 human 404636 DENND10 DENN domain containing 10 XM_017016264.2 84.1% 82.6% (many diffs)
7 human 404636 DENND10 DENN domain containing 10 NM_001303112.2 79.2% 76.7% (many diffs)
8 human 404636 DENND10 DENN domain containing 10 XM_006717849.4 68.6% 68.3% (many diffs)
9 human 404636 DENND10 DENN domain containing 10 XM_006717850.3 68.6% 68.3% (many diffs)
10 human 404636 DENND10 DENN domain containing 10 XM_006717851.3 68.6% 68.3% (many diffs)
11 human 404636 DENND10 DENN domain containing 10 XM_006717852.3 68.6% 68.3% (many diffs)
12 human 404636 DENND10 DENN domain containing 10 XM_017016265.1 68.6% 68.3% (many diffs)
13 human 404636 DENND10 DENN domain containing 10 XM_017016266.2 68.6% 68.3% (many diffs)
14 human 404636 DENND10 DENN domain containing 10 XM_017016267.1 68.6% 68.3% (many diffs)
15 human 404636 DENND10 DENN domain containing 10 NM_001303113.2 57.5% 55.3% (many diffs)
16 human 55855 DENND10P1 DENND10 pseudogene 1 NR_027141.2 42.6% 1_218del;1037C>T;1290_2508del
17 human 404636 DENND10 DENN domain containing 10 NR_130122.2 39% (many diffs)
18 mouse 67894 Fam45a family with sequence simila... NM_026437.3 88.3% 90.1% (many diffs)
19 mouse 67894 Fam45a family with sequence simila... NM_001167829.1 86.8% 88.5% (many diffs)
20 mouse 67894 Fam45a family with sequence simila... XM_006527309.3 82.1% 80.6% (many diffs)
21 mouse 67894 Fam45a family with sequence simila... NM_001347464.1 58.1% 59.9% (many diffs)
22 mouse 67894 Fam45a family with sequence simila... XM_006527311.2 58.1% 59.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1137
ORF length:
1071
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tgcggccgag ttggcggaca ctcagctgat gcttggagtc gggctgatcg
121aaaaggacac aaatggagaa gttctgtggg tgtggtgtta tccttccacg acagccacat
181taaggaacct gctgctgaga aaatgctgcc ttacagatga aaacaaactt ctccatccct
241ttgtctttgg tcagtacaga agaacatggt tttatatcac aataattgaa gttccagatt
301cttccatttt gaaaaaggtg actcattttt ctattgtcct gaccgccaaa gattttaacc
361cagagaagta tgctgccttc actaggatat tgtgtagaat gtacctgaaa catgggagcc
421cagttaaaat gatggagagt tatattgcag ttctcacaaa ggggatatgc cagagtgaag
481aaaacggctc tttccttagt aaggattttg atgcccgaaa ggcctacccg gctggctcca
541tcaaagacat tgtatctcag tttggaatgg aaactgttat cttacacaca gcactgatgc
601taaagaaaag aattgtggtg tatcacccca agatagaagc ggtccaggag ttcaccagga
661ctctgcctgc cctggtgtgg caccgacagg actggaccat ccttcactct tacgtgcacc
721tcaacgccga tgagctggaa gccctgcaga tgtgcacagg ttacgtcgct ggatttgtag
781acttggaggt gagcaacaga ccagacctct atgatgtgtt tgtgaatctg gcagagagtg
841agattaccat tgctcccctt gcaaaagagg ccatggcaat gggtaaactg cacaaagaaa
901tgggtcagct aattgttcag tctgcagaag atccagagaa atcagagagc caggttatac
961aggatattgc tctaaaaaca agagaaatct ttaccaacct agcaccgttt tcagaagttt
1021cggctgatgg agaaaagaga gtccttaatt tggaggcgct aaagcaaaaa cgatttccac
1081cagcaacaga aaacttcctt tatcatctag cagcagccga acaaatgctg aaaatctgcc
1141caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1201ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1261tta

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