Transcript: Mouse NM_001167829.1

Mus musculus family with sequence similarity 45, member A (Fam45a), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Fam45a (67894)
Length:
2345
CDS:
50..1102

Additional Resources:

NCBI RefSeq record:
NM_001167829.1
NBCI Gene record:
Fam45a (67894)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001167829.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329147 GCCGCCTTCACTAGGATATTG pLKO_005 356 CDS 100% 13.200 18.480 N Fam45a n/a
2 TRCN0000329151 TAAAGAGATTGGTCAACTAAT pLKO_005 856 CDS 100% 13.200 18.480 N Fam45a n/a
3 TRCN0000175315 CCAGCCCATAATGCTTTCATA pLKO.1 1886 3UTR 100% 5.625 7.875 N Fam45a n/a
4 TRCN0000329150 CAGACCTCTATGACGTGTTTG pLKO_005 765 CDS 100% 10.800 8.640 N Fam45a n/a
5 TRCN0000174340 CATTCTTACATGCACCTTCAT pLKO.1 689 CDS 100% 4.950 3.960 N Fam45a n/a
6 TRCN0000329221 TCCTAGTACCCATTGACATTA pLKO_005 1149 3UTR 100% 13.200 9.240 N Fam45a n/a
7 TRCN0000329222 TCTAGCAGCAGCTGAACAAAT pLKO_005 1069 CDS 100% 13.200 9.240 N Fam45a n/a
8 TRCN0000215338 CTCCATCAAAGACATTGTATC pLKO.1 520 CDS 100% 10.800 7.560 N Fam45a n/a
9 TRCN0000194105 GAAATCAGACAGCCAGGTTAT pLKO.1 901 CDS 100% 10.800 7.560 N Fam45a n/a
10 TRCN0000174458 CAATTTGGAATGGAAACTGTT pLKO.1 542 CDS 100% 4.950 3.465 N Fam45a n/a
11 TRCN0000173241 CCAGACCTCTATGACGTGTTT pLKO.1 764 CDS 100% 4.950 3.465 N Fam45a n/a
12 TRCN0000141718 GCTGGCTCCATCAAAGACATT pLKO.1 515 CDS 100% 4.950 3.465 N DENND10P1 n/a
13 TRCN0000193388 CTGCATAAAGAGATTGGTCAA pLKO.1 851 CDS 100% 4.050 2.835 N Fam45a n/a
14 TRCN0000193953 GTGTTTGTGAATCTGGCAGAT pLKO.1 779 CDS 100% 4.050 2.835 N Fam45a n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001167829.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05655 pDONR223 100% 87% 88.7% None (many diffs) n/a
2 ccsbBroad304_05655 pLX_304 0% 87% 88.7% V5 (many diffs) n/a
3 TRCN0000471589 AGATGCAGCGCCACAGGGAATCAA pLX_317 33.1% 87% 88.7% V5 (many diffs) n/a
4 ccsbBroadEn_08599 pDONR223 100% 86.8% 88.5% None (many diffs) n/a
5 ccsbBroad304_08599 pLX_304 0% 86.8% 88.5% V5 (many diffs) n/a
6 TRCN0000475565 CGACCGCATCACTAATATAACCAG pLX_317 27.7% 86.8% 88.5% V5 (many diffs) n/a
Download CSV