Construct: ORF ccsbBroad304_08641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014176.1_s304c1, BRDN0000403741
- Derived from:
- ccsbBroadEn_08641
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4ENIF1 (56478)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08641
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | NM_001164501.2 | 99.9% | 99.8% | 2474A>G |
| 2 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | NM_019843.4 | 99.9% | 99.8% | 2474A>G |
| 3 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_011530280.2 | 99.9% | 99.8% | 2474A>G |
| 4 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_017028864.2 | 99.9% | 99.8% | 2474A>G |
| 5 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_005261686.3 | 99.8% | 99.7% | 1846_1848delCAG;2477A>G |
| 6 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_005261687.3 | 99.8% | 99.7% | 1846_1848delCAG;2477A>G |
| 7 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_005261688.3 | 99.8% | 99.7% | 1846_1848delCAG;2477A>G |
| 8 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_011530281.3 | 99.8% | 99.7% | 1846_1848delCAG;2477A>G |
| 9 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_017028863.2 | 95.9% | 95.9% | 1_123del;2597A>G |
| 10 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_006724281.4 | 95.8% | 95.8% | 1_123del;1969_1971delCAG;2600A>G |
| 11 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | NM_001164502.2 | 82.1% | 82% | (many diffs) |
| 12 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XR_001755264.2 | 46.4% | (many diffs) | |
| 13 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_001166547.1 | 88.2% | 89.6% | (many diffs) |
| 14 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_023743.2 | 88.2% | 89.6% | (many diffs) |
| 15 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514841.2 | 88.2% | 89.6% | (many diffs) |
| 16 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514842.3 | 88.2% | 89.6% | (many diffs) |
| 17 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514843.3 | 88.2% | 89.6% | (many diffs) |
| 18 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_001166548.1 | 86.1% | 87.6% | (many diffs) |
| 19 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_001166549.1 | 86.1% | 87.6% | (many diffs) |
| 20 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514844.2 | 86.1% | 87.6% | (many diffs) |
| 21 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_017314806.1 | 86.1% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3021
- ORF length:
- 2955
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga taggagaagt atgggtgaaa cagaaagtgg agatgctttc cttgacctga 121 agaagcctcc tgcctccaaa tgcccccatc gctatacaaa agaagaactc ttggatataa 181 aagaactccc ccattccaaa cagaggcctt catgcctttc tgaaaaatat gacagtgatg 241 gtgtctggga ccctgagaag tggcatgcct ctctctaccc agcttcaggg cggagctcac 301 cagtggaaag tctgaagaaa gagttggata cagaccggcc ttccctggtg cgcaggatag 361 tagatccacg agagcgtgtg aaagaagatg acttagatgt tgttctcagc cctcagagac 421 ggagctttgg agggggctgc cacgtgacag ccgctgttag ctcccggcgc tcaggaagtc 481 cattagagaa agatagtgat gggcttcgtc tgcttggtgg acgtaggatt ggcagtggga 541 ggataatctc tgcccggacc tttgagaagg atcaccgtct tagcgataag gacctgcggg 601 acttgagaga cagagaccga gagagggact tcaaggacaa gcgtttcagg agagagtttg 661 gagatagtaa gcgtgtcttt ggtgagcgta gaagaaatga ttcttacaca gaagaagaac 721 cagagtggtt ctctgctgga cccacaagtc agtctgaaac catcgaactg actggctttg 781 atgataagat actagaagaa gatcacaaag ggagaaaaag aacaaggcga cggacagcct 841 ctgtgaagga aggtatagta gagtgcaatg gaggagtggc cgaagaggat gaagtggagg 901 tcatccttgc acaggagcct gcggctgatc aggaagtgcc aagggatgct gtcttgcctg 961 agcagtcccc aggagacttt gactttaatg agttctttaa ccttgataag gtgccatgct 1021 tggcttcgat gatagaagat gttttgggag aagggtcagt ctctgccagt cggttcagta 1081 ggtggttctc taacccgagc agatcaggaa gccgatccag cagtcttggg tcaacaccac 1141 atgaagagct agagagactt gcaggtctgg agcaagccat cctctctcct ggacagaact 1201 cggggaatta ctttgctcct ataccattgg aagaccatgc tgaaaataaa gtggatattt 1261 tagaaatgct acagaaagcc aaagtggatt tgaaacctct tctttccagc ctttctgcaa 1321 ataaagaaaa acttaaagaa agctcacatt caggggttgt gctttcagtg gaggaggtag 1381 aagcaggtct gaagggcttg aaggttgacc agcaagtgaa gaattcaact cccttcatgg 1441 cagaacacct agaagagacc ttgagtgccg taaccaacaa tcgacaactg aagaaagacg 1501 gagacatgac tgcgttcaac aagctagtga gcacaatgaa ggcaagtggg actttgcctt 1561 ctcagcccaa agtcagccga aaccttgaaa gccatttgat gtcccctgct gagattccag 1621 gccagcctgt ccctaagaac atcctgcagg aacttctggg tcaaccagtt cagagacctg 1681 cttcttccaa tcttctgagt ggccttatgg ggagcttgga gcctacaaca tctttactgg 1741 gccaaagagc accctctcct cccttgtcac aggtgtttca aactcgagca gcctcagctg 1801 actaccttcg cccaagaata ccatcaccaa ttggtttcac accaggacca cagcagctac 1861 tcggagatcc attccaaggc atgcgcaaac ccatgagccc catcacagcc cagatgagcc 1921 agctggagtt gcaacaggca gctttagaag ggctggcctt gccacatgac cttgctgtac 1981 aggcagcaaa cttctaccag cctggttttg gcaaaccaca ggtggacaga accagagatg 2041 gattcagaaa caggcaacag cgagtgacca agtcaccagc acccgtgcat cgagggaatt 2101 cctcttcccc tgcccctgct gcctccatca caagcatgct ttctccttcc tttaccccta 2161 cctcagtgat tcgtaagatg tacgagagca aagagaaaag caaggaggag ccagcatctg 2221 gaaaagcagc tcttggtgac agtaaagagg atactcagaa ggccagtgaa gaaaacctcc 2281 tgtcatccag ctctgtaccc agtgccgatc gagactcttc tcccactaca aattccaaac 2341 tgtcagcatt acagaggtct tcgtgttcca ccccactgtc ccaggccaac cgttacacca 2401 aagaacaaga ttatcgacct aaagcaactg ggagaaaaac acccaccttg gcatccccag 2461 ttcctacaac accttttctc cgccctgtcc accaagttcc ccttgtcccc catgtcccta 2521 tggttaggcc tgctcaccgg cttcacccag ggttggtaca gaggatgctg gcccagggag 2581 tacatccaca gcatcttcca agtttgctcc aaactggtgt gcttcctcct gggatggact 2641 tgagtcattt acagggaata tctggcccca tcctgggtca gcccttttac cctttacctg 2701 ctgctagtca ccctctctta aaccctcgtc ctggaacacc tctgcatctg gcaatggtgc 2761 aacagcagct acagcgctca gttctgcatc ctccaggctc tggttcccat gcagcagctg 2821 tcagcgttca gacaacccct cagaacgtgc ccagccggtc aggcctgccc cacatgcact 2881 cccagctgga gcatcgcccc agccagagga gcagctcccc tgtgggcctt gccaaatggt 2941 ttggctcaga tgtgctacag caacccctgc cctccatgcc cgccaaagtt atcagtgtag 3001 atgaattgga ataccgacag tgcccaactt tcttgtacaa agtggttggt aagcctatcc 3061 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 3121 ttaagtcgac aatcaacctc tggatta