Construct: ORF ccsbBroad304_08641

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014176.1_s304c1, BRDN0000403741
Derived from:
ccsbBroadEn_08641
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF4ENIF1 (56478)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08641
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56478 EIF4ENIF1 eukaryotic translation init... NM_001164501.2 99.9% 99.8% 2474A>G
2 human 56478 EIF4ENIF1 eukaryotic translation init... NM_019843.4 99.9% 99.8% 2474A>G
3 human 56478 EIF4ENIF1 eukaryotic translation init... XM_011530280.2 99.9% 99.8% 2474A>G
4 human 56478 EIF4ENIF1 eukaryotic translation init... XM_017028864.2 99.9% 99.8% 2474A>G
5 human 56478 EIF4ENIF1 eukaryotic translation init... XM_005261686.3 99.8% 99.7% 1846_1848delCAG;2477A>G
6 human 56478 EIF4ENIF1 eukaryotic translation init... XM_005261687.3 99.8% 99.7% 1846_1848delCAG;2477A>G
7 human 56478 EIF4ENIF1 eukaryotic translation init... XM_005261688.3 99.8% 99.7% 1846_1848delCAG;2477A>G
8 human 56478 EIF4ENIF1 eukaryotic translation init... XM_011530281.3 99.8% 99.7% 1846_1848delCAG;2477A>G
9 human 56478 EIF4ENIF1 eukaryotic translation init... XM_017028863.2 95.9% 95.9% 1_123del;2597A>G
10 human 56478 EIF4ENIF1 eukaryotic translation init... XM_006724281.4 95.8% 95.8% 1_123del;1969_1971delCAG;2600A>G
11 human 56478 EIF4ENIF1 eukaryotic translation init... NM_001164502.2 82.1% 82% (many diffs)
12 human 56478 EIF4ENIF1 eukaryotic translation init... XR_001755264.2 46.4% (many diffs)
13 mouse 74203 Eif4enif1 eukaryotic translation init... NM_001166547.1 88.2% 89.6% (many diffs)
14 mouse 74203 Eif4enif1 eukaryotic translation init... NM_023743.2 88.2% 89.6% (many diffs)
15 mouse 74203 Eif4enif1 eukaryotic translation init... XM_006514841.2 88.2% 89.6% (many diffs)
16 mouse 74203 Eif4enif1 eukaryotic translation init... XM_006514842.3 88.2% 89.6% (many diffs)
17 mouse 74203 Eif4enif1 eukaryotic translation init... XM_006514843.3 88.2% 89.6% (many diffs)
18 mouse 74203 Eif4enif1 eukaryotic translation init... NM_001166548.1 86.1% 87.6% (many diffs)
19 mouse 74203 Eif4enif1 eukaryotic translation init... NM_001166549.1 86.1% 87.6% (many diffs)
20 mouse 74203 Eif4enif1 eukaryotic translation init... XM_006514844.2 86.1% 87.6% (many diffs)
21 mouse 74203 Eif4enif1 eukaryotic translation init... XM_017314806.1 86.1% 87.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3021
ORF length:
2955
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga taggagaagt atgggtgaaa cagaaagtgg agatgctttc cttgacctga
121agaagcctcc tgcctccaaa tgcccccatc gctatacaaa agaagaactc ttggatataa
181aagaactccc ccattccaaa cagaggcctt catgcctttc tgaaaaatat gacagtgatg
241gtgtctggga ccctgagaag tggcatgcct ctctctaccc agcttcaggg cggagctcac
301cagtggaaag tctgaagaaa gagttggata cagaccggcc ttccctggtg cgcaggatag
361tagatccacg agagcgtgtg aaagaagatg acttagatgt tgttctcagc cctcagagac
421ggagctttgg agggggctgc cacgtgacag ccgctgttag ctcccggcgc tcaggaagtc
481cattagagaa agatagtgat gggcttcgtc tgcttggtgg acgtaggatt ggcagtggga
541ggataatctc tgcccggacc tttgagaagg atcaccgtct tagcgataag gacctgcggg
601acttgagaga cagagaccga gagagggact tcaaggacaa gcgtttcagg agagagtttg
661gagatagtaa gcgtgtcttt ggtgagcgta gaagaaatga ttcttacaca gaagaagaac
721cagagtggtt ctctgctgga cccacaagtc agtctgaaac catcgaactg actggctttg
781atgataagat actagaagaa gatcacaaag ggagaaaaag aacaaggcga cggacagcct
841ctgtgaagga aggtatagta gagtgcaatg gaggagtggc cgaagaggat gaagtggagg
901tcatccttgc acaggagcct gcggctgatc aggaagtgcc aagggatgct gtcttgcctg
961agcagtcccc aggagacttt gactttaatg agttctttaa ccttgataag gtgccatgct
1021tggcttcgat gatagaagat gttttgggag aagggtcagt ctctgccagt cggttcagta
1081ggtggttctc taacccgagc agatcaggaa gccgatccag cagtcttggg tcaacaccac
1141atgaagagct agagagactt gcaggtctgg agcaagccat cctctctcct ggacagaact
1201cggggaatta ctttgctcct ataccattgg aagaccatgc tgaaaataaa gtggatattt
1261tagaaatgct acagaaagcc aaagtggatt tgaaacctct tctttccagc ctttctgcaa
1321ataaagaaaa acttaaagaa agctcacatt caggggttgt gctttcagtg gaggaggtag
1381aagcaggtct gaagggcttg aaggttgacc agcaagtgaa gaattcaact cccttcatgg
1441cagaacacct agaagagacc ttgagtgccg taaccaacaa tcgacaactg aagaaagacg
1501gagacatgac tgcgttcaac aagctagtga gcacaatgaa ggcaagtggg actttgcctt
1561ctcagcccaa agtcagccga aaccttgaaa gccatttgat gtcccctgct gagattccag
1621gccagcctgt ccctaagaac atcctgcagg aacttctggg tcaaccagtt cagagacctg
1681cttcttccaa tcttctgagt ggccttatgg ggagcttgga gcctacaaca tctttactgg
1741gccaaagagc accctctcct cccttgtcac aggtgtttca aactcgagca gcctcagctg
1801actaccttcg cccaagaata ccatcaccaa ttggtttcac accaggacca cagcagctac
1861tcggagatcc attccaaggc atgcgcaaac ccatgagccc catcacagcc cagatgagcc
1921agctggagtt gcaacaggca gctttagaag ggctggcctt gccacatgac cttgctgtac
1981aggcagcaaa cttctaccag cctggttttg gcaaaccaca ggtggacaga accagagatg
2041gattcagaaa caggcaacag cgagtgacca agtcaccagc acccgtgcat cgagggaatt
2101cctcttcccc tgcccctgct gcctccatca caagcatgct ttctccttcc tttaccccta
2161cctcagtgat tcgtaagatg tacgagagca aagagaaaag caaggaggag ccagcatctg
2221gaaaagcagc tcttggtgac agtaaagagg atactcagaa ggccagtgaa gaaaacctcc
2281tgtcatccag ctctgtaccc agtgccgatc gagactcttc tcccactaca aattccaaac
2341tgtcagcatt acagaggtct tcgtgttcca ccccactgtc ccaggccaac cgttacacca
2401aagaacaaga ttatcgacct aaagcaactg ggagaaaaac acccaccttg gcatccccag
2461ttcctacaac accttttctc cgccctgtcc accaagttcc ccttgtcccc catgtcccta
2521tggttaggcc tgctcaccgg cttcacccag ggttggtaca gaggatgctg gcccagggag
2581tacatccaca gcatcttcca agtttgctcc aaactggtgt gcttcctcct gggatggact
2641tgagtcattt acagggaata tctggcccca tcctgggtca gcccttttac cctttacctg
2701ctgctagtca ccctctctta aaccctcgtc ctggaacacc tctgcatctg gcaatggtgc
2761aacagcagct acagcgctca gttctgcatc ctccaggctc tggttcccat gcagcagctg
2821tcagcgttca gacaacccct cagaacgtgc ccagccggtc aggcctgccc cacatgcact
2881cccagctgga gcatcgcccc agccagagga gcagctcccc tgtgggcctt gccaaatggt
2941ttggctcaga tgtgctacag caacccctgc cctccatgcc cgccaaagtt atcagtgtag
3001atgaattgga ataccgacag tgcccaactt tcttgtacaa agtggttggt aagcctatcc
3061ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
3121ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)