Transcript: Human XM_005261686.3

PREDICTED: Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EIF4ENIF1 (56478)
Length:
5625
CDS:
222..3182

Additional Resources:

NCBI RefSeq record:
XM_005261686.3
NBCI Gene record:
EIF4ENIF1 (56478)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005261686.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000427854 GGATCACCGTCTTAGCGATAA pLKO_005 725 CDS 100% 10.800 15.120 N EIF4ENIF1 n/a
2 TRCN0000194361 CCTTATGTACAGACCTGTGTA pLKO.1 3505 3UTR 100% 4.950 6.930 N Eif4enif1 n/a
3 TRCN0000340296 CCTTATGTACAGACCTGTGTA pLKO_005 3505 3UTR 100% 4.950 6.930 N Eif4enif1 n/a
4 TRCN0000153716 CGCCAAAGTTATCAGTGTAGA pLKO.1 3140 CDS 100% 4.950 6.930 N EIF4ENIF1 n/a
5 TRCN0000151549 GAAGGTATAGTAGAGTGCAAT pLKO.1 1005 CDS 100% 4.950 6.930 N EIF4ENIF1 n/a
6 TRCN0000152390 GCATTATGATGGAAGGATCTT pLKO.1 3328 3UTR 100% 4.950 6.930 N EIF4ENIF1 n/a
7 TRCN0000175849 GCTTCGATGATAGAAGATGTT pLKO.1 1179 CDS 100% 4.950 6.930 N Eif4enif1 n/a
8 TRCN0000340294 GCTTCGATGATAGAAGATGTT pLKO_005 1179 CDS 100% 4.950 6.930 N Eif4enif1 n/a
9 TRCN0000155201 GCCGTAACCAACAATCGACAA pLKO.1 1623 CDS 100% 4.050 5.670 N EIF4ENIF1 n/a
10 TRCN0000423445 CATGCTTGGCTTCGATGATAG pLKO_005 1171 CDS 100% 10.800 8.640 N EIF4ENIF1 n/a
11 TRCN0000175042 GAACAAGATTATCGACCTAAA pLKO.1 2562 CDS 100% 10.800 8.640 N Eif4enif1 n/a
12 TRCN0000154134 CCTAAGGGTTCTGAGGAAATA pLKO.1 3473 3UTR 100% 13.200 9.240 N EIF4ENIF1 n/a
13 TRCN0000153501 CAATCTTCTGAGTGGCCTTAT pLKO.1 1844 CDS 100% 10.800 7.560 N EIF4ENIF1 n/a
14 TRCN0000154055 CGTAAGATGTACGAGAGCAAA pLKO.1 2331 CDS 100% 4.950 3.465 N EIF4ENIF1 n/a
15 TRCN0000152806 GTCTGAATACAGGATGCACAA pLKO.1 3373 3UTR 100% 4.050 2.835 N EIF4ENIF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005261686.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08641 pDONR223 100% 99.8% 99.7% None 1846_1848delCAG;2477A>G n/a
2 ccsbBroad304_08641 pLX_304 0% 99.8% 99.7% V5 1846_1848delCAG;2477A>G n/a
3 ccsbBroadEn_08640 pDONR223 100% 82.2% 82.2% None 297_785del;1477_1512del;2229C>T n/a
4 ccsbBroad304_08640 pLX_304 0% 82.2% 82.2% V5 297_785del;1477_1512del;2229C>T n/a
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