Construct: ORF ccsbBroad304_08719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007485.1_s304c1, BRDN0000399189
- Derived from:
- ccsbBroadEn_08719
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX14 (57231)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08719
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57231 | SNX14 | sorting nexin 14 | NM_020468.5 | 99.9% | 99.8% | 2672C>T |
| 2 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350536.1 | 98.9% | 98.8% | 1316_1342del;2699C>T |
| 3 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350537.1 | 98.8% | 98.7% | 259_260insAGC;1313_1339del;2696C>T |
| 4 | human | 57231 | SNX14 | sorting nexin 14 | NM_001297614.2 | 95.2% | 95.1% | 416_547del;2804C>T |
| 5 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350534.1 | 95.1% | 95% | 259_260insAGC;413_544del;2801C>T |
| 6 | human | 57231 | SNX14 | sorting nexin 14 | NM_153816.6 | 94.3% | 94.2% | 416_547del;1448_1474del;2831C>T |
| 7 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350533.1 | 94.2% | 94.1% | (many diffs) |
| 8 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350542.1 | 93.2% | 93.1% | 0_1ins156;1160_1186del;2543C>T |
| 9 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350535.1 | 93% | 93% | (many diffs) |
| 10 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350543.1 | 93% | 93% | (many diffs) |
| 11 | human | 57231 | SNX14 | sorting nexin 14 | XM_024446498.1 | 93% | 93% | (many diffs) |
| 12 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350532.1 | 92.3% | 92.2% | (many diffs) |
| 13 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350541.1 | 89.3% | 89.3% | 416_547del;2363_2364ins165;2639C>T |
| 14 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350538.1 | 89.2% | 89.2% | (many diffs) |
| 15 | human | 57231 | SNX14 | sorting nexin 14 | NM_001304479.1 | 88.8% | 88.7% | (many diffs) |
| 16 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350539.1 | 88.7% | 88.6% | (many diffs) |
| 17 | human | 57231 | SNX14 | sorting nexin 14 | XM_017011090.1 | 88.7% | 88.6% | (many diffs) |
| 18 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350540.1 | 88.5% | 88.4% | (many diffs) |
| 19 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350545.1 | 84.8% | 83.5% | (many diffs) |
| 20 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350546.1 | 84.8% | 83.5% | (many diffs) |
| 21 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350544.1 | 83.7% | 83.7% | (many diffs) |
| 22 | human | 57231 | SNX14 | sorting nexin 14 | XM_011535977.2 | 83.5% | 82.7% | (many diffs) |
| 23 | human | 57231 | SNX14 | sorting nexin 14 | NR_146775.1 | 69.9% | (many diffs) | |
| 24 | human | 57231 | SNX14 | sorting nexin 14 | NR_146774.1 | 69.8% | (many diffs) | |
| 25 | human | 57231 | SNX14 | sorting nexin 14 | NR_146776.1 | 69.1% | (many diffs) | |
| 26 | human | 57231 | SNX14 | sorting nexin 14 | NR_146778.1 | 66.8% | (many diffs) | |
| 27 | human | 57231 | SNX14 | sorting nexin 14 | NR_146779.1 | 66.8% | (many diffs) | |
| 28 | human | 57231 | SNX14 | sorting nexin 14 | NR_146777.1 | 66.7% | (many diffs) | |
| 29 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350547.1 | 65% | 64.9% | 0_1ins918;398_424del;1781C>T |
| 30 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350553.1 | 61.7% | 61.7% | 0_1ins1023;1649C>T |
| 31 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350548.1 | 61.1% | 61% | 0_1ins1023;293_319del;1676C>T |
| 32 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350549.1 | 61.1% | 61% | 0_1ins1023;293_319del;1676C>T |
| 33 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350550.1 | 61.1% | 61% | 0_1ins1023;293_319del;1676C>T |
| 34 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350551.1 | 61.1% | 61% | 0_1ins1023;293_319del;1676C>T |
| 35 | human | 57231 | SNX14 | sorting nexin 14 | NM_001350552.1 | 61.1% | 61% | 0_1ins1023;293_319del;1676C>T |
| 36 | human | 57231 | SNX14 | sorting nexin 14 | XM_024446499.1 | 61.1% | 61% | 0_1ins1023;293_319del;1676C>T |
| 37 | mouse | 244962 | Snx14 | sorting nexin 14 | NM_172926.3 | 83.4% | 90.1% | (many diffs) |
| 38 | mouse | 244962 | Snx14 | sorting nexin 14 | XM_006511183.3 | 79.3% | 85.6% | (many diffs) |
| 39 | mouse | 244962 | Snx14 | sorting nexin 14 | XR_001778903.1 | 55% | (many diffs) | |
| 40 | mouse | 244962 | Snx14 | sorting nexin 14 | XR_001778902.1 | 26.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2745
- ORF length:
- 2679
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gccctgggtg cggacgatgg ggcagaagct gaagcagcgg ctgcgactgg 121 acgtgggacg cgagatctgc cgccagtacc cgctgttctg cttcctgctg ctctgtctca 181 gcgccgcctc cctgcttctt aacaggtata ttcatatttt aatgatcttc tggtcatttg 241 ttgctggagt tgtcacattc tactgctcac taggacctga ttctctctta ccaaatatat 301 tcttcacaat aaaatacaaa cccaagcagt taggacttca ggaattattt cctcaaggtc 361 atagctgtgc tgtttgtggt aaagtgaaat gtaaacgaca taggccttct ttgctacttg 421 aaaactacca gccatggcta gacctgaaaa tttcttccaa ggttgatgca tctctctcag 481 aggtggatat tccatctatt ataaccaaga aactattaaa agcagcaatg aagcatatag 541 aagtgatagt taaagccaga cagaaagtaa aaaatacaga gtttttacag caagctgctt 601 tagaagaata tggtccagag cttcatgttg ctttgagaag tcgaagagat gaattgcact 661 atttaaggaa acttactgaa ctgctttttc cttatatttt gcctcctaaa gcaacagact 721 gcagatctct gaccttactt ataagagaga ttctgtctgg ctctgtgttc cttccttctt 781 tggatttcct agctgatcca gatactgtga atcatttgct tatcatcttc atagatgaca 841 gtccacctga aaaagcaact gaaccggctt ctcctttggt tccattcttg cagaaatttg 901 cagaacctag aaataaaaag ccatctgtgc tgaagttaga attgaagcaa atcagagagc 961 aacaagatct tttatttcgt tttatgaact ttctgaaaca agaaggcgca gtgcacgtgt 1021 tgcagttttg tttgactgtg gaggaattta atgatagaat tttacgacca gaattatcaa 1081 atgatgaaat gctgtctctt catgaagaat tgcagaagat ttataaaaca tactgtttgg 1141 atgaaagtat tgacaaaatt agatttgatc ccttcattgt agaagagatt caaagaattg 1201 ctgaaggccc atacatagat gttgtgaaac ttcaaactat gagatgtctt tttgaagcat 1261 atgaacatgt tctttccctt ttggagaatg tatttactcc tatgttctgc catagtgatg 1321 agtatttcag acaactttta agaggtgcag aatcaccaac acgcaattca aaattgaaca 1381 ggaacacaca gaaaagggga gaatcatttg gaatcagcag aataggtagc aaaattaaag 1441 gagtattcaa aagtaccaca atggagggag ctatgttgcc taattatggt gtagctgaag 1501 gtgaagatga ttttattgaa gaaggtattg ttgtaatgga agatgattct ccagtggagg 1561 ctgtgagcac acctaatact ccccgaaacc ttgctgcatg gaaaattagc attccatatg 1621 tagacttttt tgaggatccc tcctctgaaa ggaaggagaa aaaagaaaga attcctgtgt 1681 tttgtattga tgttgaaaga aatgatagaa gagcagttgg acacgagcct gaacattggt 1741 ctgtctatag aagatatctt gaattctatg tacttgaatc aaaactaaca gaatttcatg 1801 gtgcatttcc tgatgcccag cttccttcta agaggatcat tggccccaaa aattatgaat 1861 tcttaaagtc aaagagggaa gagttccaag aatatctaca gaaacttctg cagcatccag 1921 aactgagtaa tagtcaactt ctggcagact ttctttcccc taatggtggg gaaacacaat 1981 ttcttgataa gatactacca gatgtaaatc ttgggaaaat tataaaatct gttcctggaa 2041 aactaatgaa agagaaaggt cagcatttgg aaccttttat catgaatttc attaattctt 2101 gtgagtctcc aaagcctaaa ccaagtagac cagaactgac cattctcagc cctacttcag 2161 aaaacaacaa gaagcttttc aatgatctgt ttaaaaataa tgcaaaccgt gctgaaaata 2221 cagagagaaa gcaaaatcag aattatttta tggaggtgat gactgtagaa ggagtctatg 2281 attacctgat gtatgtagga cgggtagttt tccaggttcc tgactggctt catcatctct 2341 taatgggaac tcgaatcctc tttaaaaaca ccctggaaat gtatactgat tactatcttc 2401 agtgtaaact agaacagcta tttcaggagc accgtttggt ctcactcata acacttctca 2461 gagatgctat attctgtgaa aacactgaac ctcgctctct ccaagataag caaaaaggag 2521 caaaacagac ttttgaagaa atgatgaatt acattccaga tctgttagtc aagtgtattg 2581 gtgaagaaac caagtatgaa agcatcagac ttctgtttga tggcttacag caaccagtac 2641 tcaacaagca gctgacttat gttttattgg acattgtgat acaggaactg tttccagagc 2701 tcaataaggt acaaaaggaa gttacctctg tgacattttg gatgtaccca actttcttgt 2761 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2821 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a