Construct: ORF ccsbBroad304_08974

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012049.1_s304c1, BRDN0000406040
Derived from:
ccsbBroadEn_08974
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG13 (79868)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08974
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_018466.6 99.7% 99.3% 495A>T
2 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001324290.2 98.6% 98.2% 243_248delAGGTAA;501A>T
3 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001039210.5 83.6% 41.2% 182_183ins59;414_415ins22
4 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001168385.3 79.5% 78.7% (many diffs)
5 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XR_001755728.1 66.1% 1_40del;535_747delinsT
6 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XR_001755727.1 63.7% 1_40del;535_775delinsT
7 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001257241.3 52.3% 50.3% (many diffs)
8 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XR_001755726.1 42.9% 1_40del;535_1149delinsT
9 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001257235.3 36.7% 36.3% 0_1ins312;183A>T
10 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001257239.3 36.7% 36.3% 0_1ins312;183A>T
11 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001257240.3 36.7% 36.3% 0_1ins312;183A>T
12 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001324291.2 36.7% 36.3% 0_1ins312;183A>T
13 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001324294.2 36.7% 36.3% 0_1ins312;183A>T
14 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_024452449.1 16.8% 14.9% (many diffs)
15 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_017029850.2 16.6% 14.7% (many diffs)
16 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_017029849.1 16.4% 14.5% (many diffs)
17 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NR_136735.2 16% (many diffs)
18 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_017029848.1 14.7% 13% (many diffs)
19 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001324292.2 14.5% 12.9% (many diffs)
20 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_011531030.2 14.2% 12.6% (many diffs)
21 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NR_033125.3 14.1% (many diffs)
22 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_006724693.3 13.7% 12.1% (many diffs)
23 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NM_001099922.3 13.5% 12% (many diffs)
24 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_017029847.1 13.4% 11.9% (many diffs)
25 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_017029846.1 13.3% 11.8% (many diffs)
26 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... XM_011531028.2 13.3% 11.7% (many diffs)
27 human 79868 ALG13 ALG13 UDP-N-acetylglucosami... NR_148693.2 13% (many diffs)
28 mouse 67574 Alg13 asparagine-linked glycosyla... NM_026247.3 85.5% 86.6% (many diffs)
29 mouse 320302 Glt28d2 glycosyltransferase 28 doma... NM_177130.3 85.2% 88.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
561
ORF length:
495
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gtgcgtgttt gttaccgtag ggaccaccag ctttgacgac ctcattgcgt
121gtgtgtcggc gcccgacagt ctgcaaaaaa tcgagagcct tggttacaac cgacttatcc
181tgcaaattgg tagaggaacg gtggtacctg aacccttcag tactgagtcg tttactctgg
241atgtttacag gtacaaggat tccttgaaag aagacattca gaaagcagat cttgttatta
301gtcacgcagg tgcaggaagc tgtttggaga ctctggaaaa aggaaagcca ctcgtagtgg
361ttataaacga aaagttgatg aacaatcatc agctggaact ggcaaagcag ctacacaaag
421agggtcatct cttctattgt acctgcagca cgcttcctgg gctgttacag tcaatggact
481tatcaacact gaaatgttat cctcctggcc agccagaaaa attttctgca tttttggata
541aagttgttgg attacaaaat tccccaactt tcttgtacaa agtggttggt aagcctatcc
601ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
661ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)