Transcript: Human NM_001257235.3

Homo sapiens ALG13 UDP-N-acetylglucosaminyltransferase subunit (ALG13), transcript variant 9, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ALG13 (79868)
Length:
2739
CDS:
383..568

Additional Resources:

NCBI RefSeq record:
NM_001257235.3
NBCI Gene record:
ALG13 (79868)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001257235.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236275 AGCCACTCGTAGTGGTTATAA pLKO_005 351 5UTR 100% 15.000 21.000 N ALG13 n/a
2 TRCN0000034823 GCCACTCGTAGTGGTTATAAA pLKO.1 352 5UTR 100% 15.000 21.000 N ALG13 n/a
3 TRCN0000236273 ACAGAGTGGAGTGGATATATT pLKO_005 1110 3UTR 100% 15.000 10.500 N ALG13 n/a
4 TRCN0000236274 TGTTACAGTCAATGGACTTAT pLKO_005 468 CDS 100% 13.200 9.240 N ALG13 n/a
5 TRCN0000034822 ACCAGCTTTGACGACCTCATT pLKO.1 80 5UTR 100% 4.950 3.465 N ALG13 n/a
6 TRCN0000034820 GCTGTTACAGTCAATGGACTT pLKO.1 466 CDS 100% 4.050 2.835 N ALG13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001257235.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08974 pDONR223 100% 36.7% 36.3% None 0_1ins312;183A>T n/a
2 ccsbBroad304_08974 pLX_304 0% 36.7% 36.3% V5 0_1ins312;183A>T n/a
3 TRCN0000468097 GAAGCAGCAAATCCCAAGTAACAG pLX_317 69.1% 36.7% 36.3% V5 0_1ins312;183A>T n/a
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