Construct: ORF ccsbBroad304_09023

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012298.1_s304c1, BRDN0000411532
Derived from:
ccsbBroadEn_09023
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDK5RAP3 (80279)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09023
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... NM_176096.3 99.9% 100% 1518G>C
2 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... NM_001278197.1 95.1% 94.9% (many diffs)
3 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... XM_011525297.1 95.1% 94.9% (many diffs)
4 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... XM_017025166.2 81.5% 81.6% 0_1ins279;1239G>C
5 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... NM_001278217.1 81% 79% (many diffs)
6 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... XM_017025164.2 81% 79% (many diffs)
7 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... XM_017025165.2 81% 79% (many diffs)
8 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... NM_001278216.1 55.4% 55.5% 0_1ins675;843G>C
9 human 80279 CDK5RAP3 CDK5 regulatory subunit ass... NM_001278198.2 51.2% 50.4% (many diffs)
10 mouse 80280 Cdk5rap3 CDK5 regulatory subunit ass... NM_030248.2 86.9% 87.9% (many diffs)
11 mouse 80280 Cdk5rap3 CDK5 regulatory subunit ass... XM_006534526.3 78.5% 79.2% (many diffs)
12 mouse 80280 Cdk5rap3 CDK5 regulatory subunit ass... XM_011249319.2 78.5% 79.2% (many diffs)
13 mouse 80280 Cdk5rap3 CDK5 regulatory subunit ass... XM_011249320.2 78.5% 79.2% (many diffs)
14 mouse 80280 Cdk5rap3 CDK5 regulatory subunit ass... NM_001308183.1 67.3% 67.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1584
ORF length:
1518
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ggaccatcag cacgtgccca tcgacatcca gaccagcaag ctgctcgatt
121ggctggtgga cagaaggcac tgcagcctga aatggcagag tctggtgctg acgatccgcg
181agaagatcaa tgctgccatc caggacatgc cagagagcga agagatcgcc cagctgctgt
241ctgggtccta cattcactac tttcactgcc taagaatcct ggaccttctc aaaggcacag
301aggcctccac gaagaatatt tttggccgat actcttcaca gcggatgaag gattggcagg
361agattatagc tctgtatgag aaggacaaca cctacttagt ggaactctct agcctcctgg
421ttcggaatgt caactatgag atcccctcac tgaagaagca gattgccaag tgccagcagc
481tgcagcaaga atacagccgc aaggaggagg agtgccaggc aggggctgcc gagatgcggg
541agcagttcta ccactcctgc aagcagtatg gcatcacggg cgaaaatgtc cgaggagaac
601tgctggccct ggtgaaggac ctgccgagtc agctggctga gattggggca gcggctcagc
661agtccctggg ggaagccatt gacgtgtacc aggcgtctgt ggggtttgtg tgtgagagcc
721ccacagagca ggtgttgcca atgctgcggt tcgtgcagaa gcggggaaac tcaacggtgt
781acgagtggag gacagggaca gagccctctg tggtggaacg accccacctc gaggagcttc
841ctgagcaggt ggcagaagat gcgattgact ggggcgactt tggggtagag gcagtgtctg
901aggggactga ctctggcatc tctgccgagg ctgctggaat cgactggggc atcttcccgg
961aatcagattc aaaggatcct ggaggtgatg ggatagactg gggagacgat gctgttgctt
1021tgcagatcac agtgctggaa gcaggaaccc aggctccaga aggtgttgcc aggggcccag
1081atgccctgac actgcttgaa tacactgaga cccggaatca gttccttgat gagctcatgg
1141agcttgagat cttcttagcc cagagagcag tggagttgag tgaggaggca gatgtcctgt
1201ctgtgagcca gttccagctg gctccagcca tcctgcaggg ccagaccaaa gagaagatgg
1261ttaccatggt gtcagtgctg gaggatctga ttggcaagct taccagtctt cagctgcaac
1321acctgtttat gatcctggcc tcaccaaggt atgtggaccg agtgactgaa ttcctccagc
1381aaaagctgaa gcagtcccag ctgctggctt tgaagaaaga gctgatggtg cagaagcagc
1441aggaggcact tgaggagcag gcggctctgg agcctaagct ggacctgcta ctggagaaga
1501ccaaggagct gcagaagctg attgaagctg acatctccaa gaggtacagc gggcgccctg
1561tgaacctgat gggaacctct ctctgcccaa ctttcttgta caaagtggtt ggtaagccta
1621tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1681gcgttaagtc gacaatcaac ctctggatta

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