Construct: ORF ccsbBroad304_09023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012298.1_s304c1, BRDN0000411532
- Derived from:
- ccsbBroadEn_09023
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK5RAP3 (80279)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09023
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | NM_176096.3 | 99.9% | 100% | 1518G>C |
| 2 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | NM_001278197.1 | 95.1% | 94.9% | (many diffs) |
| 3 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | XM_011525297.1 | 95.1% | 94.9% | (many diffs) |
| 4 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | XM_017025166.2 | 81.5% | 81.6% | 0_1ins279;1239G>C |
| 5 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | NM_001278217.1 | 81% | 79% | (many diffs) |
| 6 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | XM_017025164.2 | 81% | 79% | (many diffs) |
| 7 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | XM_017025165.2 | 81% | 79% | (many diffs) |
| 8 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | NM_001278216.1 | 55.4% | 55.5% | 0_1ins675;843G>C |
| 9 | human | 80279 | CDK5RAP3 | CDK5 regulatory subunit ass... | NM_001278198.2 | 51.2% | 50.4% | (many diffs) |
| 10 | mouse | 80280 | Cdk5rap3 | CDK5 regulatory subunit ass... | NM_030248.2 | 86.9% | 87.9% | (many diffs) |
| 11 | mouse | 80280 | Cdk5rap3 | CDK5 regulatory subunit ass... | XM_006534526.3 | 78.5% | 79.2% | (many diffs) |
| 12 | mouse | 80280 | Cdk5rap3 | CDK5 regulatory subunit ass... | XM_011249319.2 | 78.5% | 79.2% | (many diffs) |
| 13 | mouse | 80280 | Cdk5rap3 | CDK5 regulatory subunit ass... | XM_011249320.2 | 78.5% | 79.2% | (many diffs) |
| 14 | mouse | 80280 | Cdk5rap3 | CDK5 regulatory subunit ass... | NM_001308183.1 | 67.3% | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1584
- ORF length:
- 1518
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaccatcag cacgtgccca tcgacatcca gaccagcaag ctgctcgatt 121 ggctggtgga cagaaggcac tgcagcctga aatggcagag tctggtgctg acgatccgcg 181 agaagatcaa tgctgccatc caggacatgc cagagagcga agagatcgcc cagctgctgt 241 ctgggtccta cattcactac tttcactgcc taagaatcct ggaccttctc aaaggcacag 301 aggcctccac gaagaatatt tttggccgat actcttcaca gcggatgaag gattggcagg 361 agattatagc tctgtatgag aaggacaaca cctacttagt ggaactctct agcctcctgg 421 ttcggaatgt caactatgag atcccctcac tgaagaagca gattgccaag tgccagcagc 481 tgcagcaaga atacagccgc aaggaggagg agtgccaggc aggggctgcc gagatgcggg 541 agcagttcta ccactcctgc aagcagtatg gcatcacggg cgaaaatgtc cgaggagaac 601 tgctggccct ggtgaaggac ctgccgagtc agctggctga gattggggca gcggctcagc 661 agtccctggg ggaagccatt gacgtgtacc aggcgtctgt ggggtttgtg tgtgagagcc 721 ccacagagca ggtgttgcca atgctgcggt tcgtgcagaa gcggggaaac tcaacggtgt 781 acgagtggag gacagggaca gagccctctg tggtggaacg accccacctc gaggagcttc 841 ctgagcaggt ggcagaagat gcgattgact ggggcgactt tggggtagag gcagtgtctg 901 aggggactga ctctggcatc tctgccgagg ctgctggaat cgactggggc atcttcccgg 961 aatcagattc aaaggatcct ggaggtgatg ggatagactg gggagacgat gctgttgctt 1021 tgcagatcac agtgctggaa gcaggaaccc aggctccaga aggtgttgcc aggggcccag 1081 atgccctgac actgcttgaa tacactgaga cccggaatca gttccttgat gagctcatgg 1141 agcttgagat cttcttagcc cagagagcag tggagttgag tgaggaggca gatgtcctgt 1201 ctgtgagcca gttccagctg gctccagcca tcctgcaggg ccagaccaaa gagaagatgg 1261 ttaccatggt gtcagtgctg gaggatctga ttggcaagct taccagtctt cagctgcaac 1321 acctgtttat gatcctggcc tcaccaaggt atgtggaccg agtgactgaa ttcctccagc 1381 aaaagctgaa gcagtcccag ctgctggctt tgaagaaaga gctgatggtg cagaagcagc 1441 aggaggcact tgaggagcag gcggctctgg agcctaagct ggacctgcta ctggagaaga 1501 ccaaggagct gcagaagctg attgaagctg acatctccaa gaggtacagc gggcgccctg 1561 tgaacctgat gggaacctct ctctgcccaa ctttcttgta caaagtggtt ggtaagccta 1621 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1681 gcgttaagtc gacaatcaac ctctggatta