Transcript: Human NM_001278198.2

Homo sapiens CDK5 regulatory subunit associated protein 3 (CDK5RAP3), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
CDK5RAP3 (80279)
Length:
2632
CDS:
1635..2585

Additional Resources:

NCBI RefSeq record:
NM_001278198.2
NBCI Gene record:
CDK5RAP3 (80279)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001278198.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000135914 GATTGGCAGGAGATTATAGCT pLKO.1 320 5UTR 100% 3.000 2.400 N CDK5RAP3 n/a
2 TRCN0000330499 CGATCCGCGAGAAGATCAATG pLKO_005 141 5UTR 100% 10.800 7.560 N CDK5RAP3 n/a
3 TRCN0000353742 GGTCCTACATTCACTACTTTC pLKO_005 213 5UTR 100% 10.800 7.560 N CDK5RAP3 n/a
4 TRCN0000135398 GTTCTGAGATACCAGGCTTAT pLKO.1 423 5UTR 100% 10.800 7.560 N CDK5RAP3 n/a
5 TRCN0000134828 CAACACCTACTTAGGTAAAGT pLKO.1 355 5UTR 100% 5.625 3.938 N CDK5RAP3 n/a
6 TRCN0000135657 GAGAAGGACAACACCTACTTA pLKO.1 347 5UTR 100% 5.625 3.938 N CDK5RAP3 n/a
7 TRCN0000136838 CCCTCACTGAAGAAGCAGATT pLKO.1 1123 5UTR 100% 4.950 3.465 N CDK5RAP3 n/a
8 TRCN0000330498 CCCTCACTGAAGAAGCAGATT pLKO_005 1123 5UTR 100% 4.950 3.465 N CDK5RAP3 n/a
9 TRCN0000136052 GCAGGAGATTATAGCTCTGTA pLKO.1 325 5UTR 100% 4.950 3.465 N CDK5RAP3 n/a
10 TRCN0000330497 GCAGGAGATTATAGCTCTGTA pLKO_005 325 5UTR 100% 4.950 3.465 N CDK5RAP3 n/a
11 TRCN0000136898 CTTCACAGCGGATGAAGGATT pLKO.1 303 5UTR 100% 0.495 0.347 N CDK5RAP3 n/a
12 TRCN0000137232 GAGGCCTCCACGAAGAATATT pLKO.1 269 5UTR 100% 15.000 9.000 N CDK5RAP3 n/a
13 TRCN0000330496 GAGGCCTCCACGAAGAATATT pLKO_005 269 5UTR 100% 15.000 9.000 N CDK5RAP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001278198.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09023 pDONR223 100% 51.2% 50.4% None (many diffs) n/a
2 ccsbBroad304_09023 pLX_304 0% 51.2% 50.4% V5 (many diffs) n/a
3 TRCN0000480634 TTTTTAAGTTTGGGACTATATTCT pLX_317 23% 51.2% 50.4% V5 (many diffs) n/a
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