Construct: ORF ccsbBroad304_09267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014752.1_s304c1, BRDN0000461425
- Derived from:
- ccsbBroadEn_09267
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIXDC1 (85458)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09267
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85458 | DIXDC1 | DIX domain containing 1 | NM_033425.4 | 99.8% | 99.7% | 1274G>T;1314T>A |
| 2 | human | 85458 | DIXDC1 | DIX domain containing 1 | XM_017018468.1 | 82.2% | 82.2% | 0_1ins249;1025G>T;1065T>A |
| 3 | human | 85458 | DIXDC1 | DIX domain containing 1 | XM_017018469.1 | 80.8% | 80% | (many diffs) |
| 4 | human | 85458 | DIXDC1 | DIX domain containing 1 | XM_024448743.1 | 72.6% | 71.9% | (many diffs) |
| 5 | human | 85458 | DIXDC1 | DIX domain containing 1 | XM_024448742.1 | 72.5% | 71.8% | (many diffs) |
| 6 | human | 85458 | DIXDC1 | DIX domain containing 1 | XM_017018466.2 | 68.7% | 68% | (many diffs) |
| 7 | human | 85458 | DIXDC1 | DIX domain containing 1 | XM_017018467.1 | 68.7% | 68% | (many diffs) |
| 8 | human | 85458 | DIXDC1 | DIX domain containing 1 | NM_001037954.4 | 68.6% | 67.9% | (many diffs) |
| 9 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_006510456.3 | 88.1% | 90.2% | (many diffs) |
| 10 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_017313441.1 | 83.8% | 86.2% | (many diffs) |
| 11 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_006510457.3 | 72.6% | 74.8% | (many diffs) |
| 12 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_006510454.3 | 60.7% | 61.4% | (many diffs) |
| 13 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | NM_001311069.1 | 58.5% | 59.2% | (many diffs) |
| 14 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_006510451.3 | 58.5% | 59.2% | (many diffs) |
| 15 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_017313439.1 | 58.5% | 59.2% | (many diffs) |
| 16 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_017313440.1 | 58.5% | 59.2% | (many diffs) |
| 17 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | NM_178118.2 | 58.5% | 59.2% | (many diffs) |
| 18 | mouse | 330938 | Dixdc1 | DIX domain containing 1 | XM_006510455.3 | 55.5% | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1485
- ORF length:
- 1416
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagggacg caagtcaaat gcctgacttc acccagtcca atccacagtg 121 caaagagcga gtccattata acccagtcag aagagaaggc agattttgtg attattcccg 181 ctgaaggaat agagaacaga acagaaggga cagattctcc attatctcga gactggcggc 241 cagggagccc tggaacctat ctggagacct catgggaaga acagctgttg gaacaacaag 301 aatatttaga aaaagaaatg gaggaagcaa agaaaatgat atcaggacta caggccttac 361 tgctcaatgg atccttacct gaagatgaac aggagaggcc cttggccctc tgtgaaccag 421 gtgtcaatcc cgaggaacaa ctgattataa tccaaagtcg tctggatcag agtatggagg 481 agaatcagga cttaaagaag gaactgctga aatgtaaaca agaagccaga aacttacagg 541 ggataaagga tgccttgcag cagagattga ctcagcagga cacatctgtt cttcagctca 601 aacaagagct actgagggca aatatggaca aagatgagct gcacaaccag aatgtggatc 661 tgcagaggaa gctagatgag aggaaccggc tcttgggaga atataaaaaa gagctggggc 721 agaaggatcg ccttcttcag cagcaccagg ccaagttaga agaagcactc cggaaactct 781 ctgatgtcag ttaccaccag gtggatctag agcgagagct agaacacaaa gatgtcctct 841 tggctcactg tatgaaaaga gaggcagatg aggcgaccaa ctacaacagt cacaactctc 901 aaagcaatgg ttttctcctt ccaacggcag gaaaaggagc tacttcagtc agcaacagag 961 ggaccagcga cctgcagctt gttcgagatg ctctccgcag cctgcgcaac agcttcagtg 1021 gccacgatcc tcagcaccac actattgaca gcttggagca gggcatttct agcctcatgg 1081 agcgcctgca tgttatggag acgcagaaga aacaagaaag aaaggttcgg gtcaagtcac 1141 ccagaactca agtaggtagt gaataccggg agtcctggcc ccctaactca aagttgcctc 1201 actcacagag ctctccaact gtcagcagca cctgtactaa agtgctctat ttcactgacc 1261 ggtcacttac gcccttcatg gtcaatatac caaagaggtt ggaggaggtg acgttaaagg 1321 attttaaagc agctattgat ctggaaggaa atcaccggta tcacttcaaa gcactggatc 1381 cagagtttgg cactgtcaaa gaggagattt tccatgatga tgatgccatc cctggatggg 1441 aagggaaaat tgtagcttgg gtggaagaag accatggaga gaatttgcca actttcttgt 1501 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1561 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a