Construct: ORF ccsbBroad304_09564
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015582.1_s304c1, BRDN0000461493
- Derived from:
- ccsbBroadEn_09564
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM50 (135892)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09564
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135892 | TRIM50 | tripartite motif containing 50 | NM_001281451.1 | 100% | 100% | |
| 2 | human | 135892 | TRIM50 | tripartite motif containing 50 | NM_178125.3 | 100% | 100% | |
| 3 | human | 135892 | TRIM50 | tripartite motif containing 50 | NM_001281450.1 | 99.7% | 99.7% | 725_726insGAA |
| 4 | human | 135892 | TRIM50 | tripartite motif containing 50 | XM_011515789.2 | 99.7% | 99.7% | 725_726insGAA |
| 5 | human | 135892 | TRIM50 | tripartite motif containing 50 | XM_011515787.1 | 61.6% | 60.3% | (many diffs) |
| 6 | human | 135892 | TRIM50 | tripartite motif containing 50 | XM_011515788.2 | 57% | 57% | 0_1ins627 |
| 7 | human | 375593 | TRIM73 | tripartite motif containing 73 | NM_198924.3 | 49.4% | 47.8% | (many diffs) |
| 8 | human | 378108 | TRIM74 | tripartite motif containing 74 | NM_001317815.1 | 49.2% | 47.6% | (many diffs) |
| 9 | human | 378108 | TRIM74 | tripartite motif containing 74 | NM_198853.3 | 49.2% | 47.6% | (many diffs) |
| 10 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_011516187.3 | 49.2% | 47.6% | (many diffs) |
| 11 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_011516189.2 | 49% | 47.4% | (many diffs) |
| 12 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_011516190.2 | 44.2% | 39.2% | (many diffs) |
| 13 | mouse | 215061 | Trim50 | tripartite motif-containing 50 | NM_001346745.1 | 82.8% | 87.6% | (many diffs) |
| 14 | mouse | 215061 | Trim50 | tripartite motif-containing 50 | XM_006504411.3 | 82.8% | 87.6% | (many diffs) |
| 15 | mouse | 215061 | Trim50 | tripartite motif-containing 50 | NM_178240.2 | 82.6% | 87.4% | (many diffs) |
| 16 | mouse | 215061 | Trim50 | tripartite motif-containing 50 | XM_006504412.3 | 82.6% | 87.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1530
- ORF length:
- 1461
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttggcag gtgagcctgc cagagctgga ggaccggctt cagtgtccca 121 tctgcctgga ggtcttcaag gagcccctga tgctgcagtg tggccactct tactgcaagg 181 gctgcctggt ttccctgtcc tgccacctgg atgccgagct gcgctgcccc gtgtgccggc 241 aggcggtgga cggcagcagc tccctgccca acgtctccct ggccagggtg atcgaagccc 301 tgaggctccc tggggacccg gagcccaagg tctgcgtgca ccaccggaac ccgctcagcc 361 ttttctgcga gaaggaccag gagctcatct gtggcctctg cggtctgctg ggctcccacc 421 aacaccaccc ggtcacgccc gtctccaccg tctacagccg catgaaggag gagctcgcag 481 ccctcatctc tgagctgaag caggagcaga aaaaggtgga tgagctcatc gccaaactgg 541 tgaacaaccg gacccgaatc gtcaatgagt cggatgtctt cagctgggtg atccgccgcg 601 agttccagga gctgcaccac ctggtggatg aggagaaggc ccgctgcctg gaggggatag 661 ggggtcacac ccgtggcctg gtggcctccc tggacatgca gctggagcag gcccagggaa 721 cccgggagcg gctggcccaa gccgagtgtg tgctggaaca gttcggcaat gaggaccacc 781 acaagttcat ccggaagttc cactccatgg cctccagagc agagatgccg caggcccggc 841 ccttagaagg cgcattcagc cccatctcct tcaagccagg cctccaccag gctgacatca 901 agctgaccgt gtggaaaagg ctcttccgga aagttttgcc agccccggag cctctcaagt 961 tggaccctgc cactgcccac ccactcctgg agctctccaa gggcaacacg gtggtgcagt 1021 gcgggcttct ggcccagcgg cgagccagcc agcctgagcg cttcgactac agcacctgcg 1081 tcctggccag ccgcggcttc tcctgcggcc gccactactg ggaggtggtg gtgggcagca 1141 agagcgactg gcgcctgggg gtcatcaagg gcacagccag ccgtaagggc aagctgaaca 1201 ggtcccccga gcacggcgtg tggctgatcg gcctgaagga gggccgggtg tacgaagcct 1261 ttgcctgccc ccgggtaccc ctgcccgtgg ccggccaccc ccaccgcatc gggctctacc 1321 tgcactatga gcagggcgaa ctcaccttct tcgatgccga ccgccccgat gacctgcggc 1381 cgctctacac cttccaggcc gacttccagg gcaagctcta ccccatcctg gacacctgct 1441 ggcacgagag gggcagcaac tcgctgccca tggtgctgcc cccgcccagc gggcctggcc 1501 ccctcagccc cgagcagccc accaagctgt tgccaacttt cttgtacaaa gtggttggta 1561 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1621 ggtggcgcgt taagtcgaca atcaacctct ggatta