Construct: ORF ccsbBroad304_09703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014590.1_s304c1, BRDN0000403819
- Derived from:
- ccsbBroadEn_09703
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF145 (153830)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09703
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153830 | RNF145 | ring finger protein 145 | NM_001199383.2 | 99.8% | 99.5% | 26C>T;1148T>C;1970C>T |
| 2 | human | 153830 | RNF145 | ring finger protein 145 | XM_017009138.2 | 99.8% | 99.5% | 26C>T;1148T>C;1970C>T |
| 3 | human | 153830 | RNF145 | ring finger protein 145 | NM_001199382.1 | 97.7% | 97.4% | (many diffs) |
| 4 | human | 153830 | RNF145 | ring finger protein 145 | XM_024454383.1 | 97.4% | 97.2% | (many diffs) |
| 5 | human | 153830 | RNF145 | ring finger protein 145 | NM_001199381.2 | 97.3% | 97% | (many diffs) |
| 6 | human | 153830 | RNF145 | ring finger protein 145 | NM_144726.2 | 95.8% | 95.5% | (many diffs) |
| 7 | human | 153830 | RNF145 | ring finger protein 145 | NM_001199380.1 | 95.5% | 95.2% | (many diffs) |
| 8 | mouse | 74315 | Rnf145 | ring finger protein 145 | NM_028862.3 | 85.7% | 93.6% | (many diffs) |
| 9 | mouse | 74315 | Rnf145 | ring finger protein 145 | XM_006534356.2 | 85.7% | 93.6% | (many diffs) |
| 10 | mouse | 74315 | Rnf145 | ring finger protein 145 | XM_006534357.1 | 85.7% | 93.6% | (many diffs) |
| 11 | mouse | 74315 | Rnf145 | ring finger protein 145 | XM_006534358.3 | 85.7% | 93.6% | (many diffs) |
| 12 | mouse | 74315 | Rnf145 | ring finger protein 145 | XM_006534359.3 | 70.7% | 77.3% | (many diffs) |
| 13 | mouse | 74315 | Rnf145 | ring finger protein 145 | NM_001166553.1 | 34.9% | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2055
- ORF length:
- 1989
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaaaggag aaactggagg tagtgttaaa tgtggccctg agggtgccaa 121 gcatcatgct gttggatgtc ctgtacagat gggatgtcag ctcctttttc cagcagatcc 181 aaagaagtag ccttagtaat aaccctcttt tccagtataa gtatttggct cttaatatgc 241 attatgtagg ttatatctta agtgtggtgc tgctaacatt gcccaggcag catctggttc 301 agctttatct atattttttg actgctctgc tcctctatgc tggacatcaa atttccaggg 361 actatgttcg gagtgaactg gagtttgcct atgagggacc aatgtattta gaacctctct 421 ctatgaatcg gtttaccaca gccttaatag gtcagttggt ggtgtgtact ttatgctcct 481 gtgtcatgaa aacaaagcag atttggctgt tttcagctca catgcttcct ctgctagcac 541 gactctgcct tgttcctttg gagacaattg ttatcatcaa taaatttgct atgattttta 601 ctggattgga agttctctat tttcttgggt ctaatctttt ggtaccttat aaccttgcta 661 aatctgcata cagagaattg gttcaggtag tggaggtata tggccttctc gccttgggaa 721 tgtccctgtg gaatcaactg gtagtccctg ttcttttcat ggttttctgg ctcgtcttat 781 ttgctcttca gatttactcc tatttcagta ctcgagatca gcctgcatca cgtgagaggc 841 ttcttttcct ttttctgaca agtattgcgg aatgctgcag cactccttac tctcttttgg 901 gtttggtctt cacggtttct tttgttgcct tgggtgttct cacactctgc aagttttact 961 tgcagggtta tcgagctttc atgaatgatc ctgccatgaa tcggggcatg acagaaggag 1021 taacgctgtt aatcctggca gtgcagactg ggctgataga actgcaggtt gttcatcggg 1081 cattcttgct cagtattatc cttttcattg tcgtagcttc tatcctacag tctatgttag 1141 aaattgcaga tcctattgtt ttggcactgg gagcatctag agacaagagc ttgtggaaac 1201 acttccgtgc tgcaagcctt tgtttatttt tattggtatt ccctgcttat atggcttata 1261 tgatttgcca gtttttccac atggattttt ggcttcttat cattatttcc agcagcattc 1321 ttacctctct tcaggttctg ggaacacttt ttatttatgt cttatttatg gttgaggaat 1381 tcagaaaaga gccagtggaa aacatggatg atgtcatcta ctatgtgaat ggcacttacc 1441 gcctgctgga gtttcttgtg gccctctgtg tggtggccta tggcgtctca gagaccatct 1501 ttggagaatg gacagtgatg ggctcaatga tcatcttcat tcattcctac tataacgtgt 1561 ggcttcgggc ccagctgggg tggaagagct ttcttctccg cagggatgct gtgaataaga 1621 ttaaatcgtt acccattgct acgaaagagc agcttgagaa acacaatgat atttgtgcca 1681 tctgttatca ggacatgaaa tctgctgtga tcacgccttg cagtcatttt ttccatgcag 1741 gctgtcttaa gaaatggctg tatgtccagg agacctgccc tctgtgccac tgccatctga 1801 aaaactcctc ccagcttcca ggattaggaa ctgagccagt tctacagcct catgctggag 1861 ctgagcaaaa cgtcatgttt caggaaggta ctgaaccccc aggccaggag catactccag 1921 ggaccaggat acaggaaggt tccagggaca ataatgagta cattgccaga cgaccagata 1981 accaggaagg ggcttttgac cccaaagaat atcctcacag tgcgaaagat gaagtacatc 2041 ctgttgaatc agcctaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 2101 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2161 cgacaatcaa cctctggatt a