Construct: ORF ccsbBroad304_09724
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016947.1_s304c1, BRDN0000410813
- Derived from:
- ccsbBroadEn_09724
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSAG1 (158511)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09724
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158511 | CSAG1 | chondrosarcoma associated g... | NM_001102576.3 | 100% | 100% | |
| 2 | human | 158511 | CSAG1 | chondrosarcoma associated g... | NM_153478.3 | 100% | 100% | |
| 3 | human | 102723547 | CSAG2 | CSAG family member 2 | XM_017030023.1 | 66.3% | 60% | (many diffs) |
| 4 | human | 389903 | CSAG3 | CSAG family member 3 | NM_001129828.3 | 66% | 59% | (many diffs) |
| 5 | human | 102723547 | CSAG2 | CSAG family member 2 | XM_006724857.3 | 57.5% | 51.9% | (many diffs) |
| 6 | human | 389903 | CSAG3 | CSAG family member 3 | NM_001129826.3 | 57.2% | 51.1% | (many diffs) |
| 7 | human | 389903 | CSAG3 | CSAG family member 3 | XM_024452379.1 | 57.2% | 51.1% | (many diffs) |
| 8 | human | 100130935 | CSAG4 | CSAG family member 4 (pseud... | NR_073432.1 | 28.4% | (many diffs) | |
| 9 | human | 102723547 | CSAG2 | CSAG family member 2 | XR_938529.2 | 28.1% | (many diffs) | |
| 10 | human | 389903 | CSAG3 | CSAG family member 3 | NR_130638.1 | 27.1% | (many diffs) | |
| 11 | human | 102723547 | CSAG2 | CSAG family member 2 | XR_938528.2 | 17.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 300
- ORF length:
- 234
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggcgactaca gcctgctggc ctgccttcac tgtcctgggg gaagctcggg 121 gagaccaggt ggactggagt agactgtaca gagacactgg tctggtgaag atgtccagga 181 aaccacgagc ctccagccca ttttccaaca accacccatc aacaccaaag aggttcccaa 241 gacaacccag aagggaaaag ggacccgtca aggaagttcc aggaacaaaa ggctctccct 301 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 361 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 421 ggatta