Construct: ORF ccsbBroad304_09809

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004595.1_s304c1, BRDN0000400607
Derived from:
ccsbBroadEn_09809
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DHFR2 (200895)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09809
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 200895 DHFR2 dihydrofolate reductase 2 NM_001195643.2 99.8% 100% 240C>A
2 human 200895 DHFR2 dihydrofolate reductase 2 NM_176815.5 99.8% 100% 240C>A
3 human 200895 DHFR2 dihydrofolate reductase 2 XM_011512537.3 99.8% 100% 240C>A
4 human 1719 DHFR dihydrofolate reductase NM_000791.4 94.6% 91.9% (many diffs)
5 human 1719 DHFR dihydrofolate reductase NM_001290354.2 68.6% 66.3% (many diffs)
6 human 1719 DHFR dihydrofolate reductase NM_001290357.2 65.5% 61.4% (many diffs)
7 human 1720 DHFRP3 dihydrofolate reductase pse... NR_033423.1 51.9% (many diffs)
8 mouse 13361 Dhfr dihydrofolate reductase NM_010049.3 87% 86.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
627
ORF length:
561
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt tcttttgcta aactgcatcg tcgctgtgtc ccaaaacatg ggcatcggca
121agaacgggga cctgcccagg ccgccgctca ggaatgaatt caggtatttc cagagaatga
181ccacaacttc ttcagtagag ggtaaacaga atctggtgat tatgggtagg aagacctggt
241tctccattcc tgagaagaat cgacctttaa aggatagaat taatttagtt ctcagcagag
301aactaaagga acctccacaa ggagctcatt ttcttgccag aagtttggat gatgccttaa
361aacttactga acgaccagaa ttagcaaata aagtagacat gatttggata gttggtggca
421gttctgttta taaggaagcc atgaatcacc taggccatct taaactattt gtgacaagga
481tcatgcagga ctttgaaagt gacacgtttt tttcagaaat tgacttggag aaatataaac
541ttctgcctga atacccaggt gttctctctg atgtccagga ggggaaacac atcaagtaca
601aatttgaagt atgtgagaag gatgattacc caactttctt gtacaaagtg gttggtaagc
661ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
721ggcgcgttaa gtcgacaatc aacctctgga tta

Download FASTA (ORF) (Full)