Transcript: Human NM_176815.5

Homo sapiens dihydrofolate reductase 2 (DHFR2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
DHFR2 (200895)
Length:
3856
CDS:
267..830

Additional Resources:

NCBI RefSeq record:
NM_176815.5
NBCI Gene record:
DHFR2 (200895)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_176815.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000160428 CCTCACTAATCTTGGCTATAT pLKO.1 960 3UTR 100% 13.200 10.560 N DHFR2 n/a
2 TRCN0000165880 GCCCTGTCTCTACAGAAGATA pLKO.1 2371 3UTR 100% 5.625 3.938 N DHFR2 n/a
3 TRCN0000158783 GAAGTATGTGAGAAGGATGAT pLKO.1 807 CDS 100% 4.950 3.465 N DHFR2 n/a
4 TRCN0000160743 CAACTTCTTCAGTAGAGGGTA pLKO.1 385 CDS 100% 2.640 1.848 N DHFR2 n/a
5 TRCN0000158432 CGACCAGAATTAGCAAATAAA pLKO.1 573 CDS 100% 15.000 9.000 N DHFR2 n/a
6 TRCN0000160583 CCTAGGCCATCTTAAACTATT pLKO.1 650 CDS 100% 13.200 7.920 N DHFR2 n/a
7 TRCN0000159962 GAACGACCAGAATTAGCAAAT pLKO.1 570 CDS 100% 10.800 6.480 N DHFR2 n/a
8 TRCN0000159605 GAAGTTTGGATGATGCCTTAA pLKO.1 541 CDS 100% 10.800 6.480 N DHFR2 n/a
9 TRCN0000159864 GTAGACATGATTTGGATAGTT pLKO.1 594 CDS 100% 5.625 3.375 N DHFR2 n/a
10 TRCN0000158867 GCAGTTTATTTGCTAGGTCAT pLKO.1 1367 3UTR 100% 4.050 2.430 N DHFR2 n/a
11 TRCN0000422184 ACCTCCACAAGGAGCTCATTT pLKO_005 512 CDS 100% 13.200 6.600 Y DHFR n/a
12 TRCN0000420418 AGTTGGTGGCAGTTCTGTTTA pLKO_005 611 CDS 100% 13.200 6.600 Y DHFR n/a
13 TRCN0000427080 GTAAACAGAATCTGGTGATTA pLKO_005 403 CDS 100% 13.200 6.600 Y DHFR n/a
14 TRCN0000039000 CCTGAGAAGAATCGACCTTTA pLKO.1 450 CDS 100% 10.800 5.400 Y DHFR n/a
15 TRCN0000161799 CCTGAGAAGAATCGACCTTTA pLKO.1 450 CDS 100% 10.800 5.400 Y DHFR2 n/a
16 TRCN0000042194 CTGGTGATTATGGGTAGGAAA pLKO.1 414 CDS 100% 4.950 2.970 N Dhfr n/a
17 TRCN0000325290 CTGGTGATTATGGGTAGGAAA pLKO_005 414 CDS 100% 4.950 2.970 N Dhfr n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_176815.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09809 pDONR223 100% 99.8% 100% None 240C>A n/a
2 ccsbBroad304_09809 pLX_304 0% 99.8% 100% V5 240C>A n/a
3 TRCN0000478413 TATGTCCAACATCTCACTTAAACT pLX_317 66.6% 99.8% 100% V5 240C>A n/a
4 ccsbBroadEn_00443 pDONR223 100% 94.8% 91.9% None (many diffs) n/a
5 ccsbBroad304_00443 pLX_304 0% 94.8% 91.9% V5 (many diffs) n/a
6 TRCN0000478285 GCAGTACTGAGTGTACAAGCGTAG pLX_317 55.7% 94.8% 91.9% V5 (many diffs) n/a
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