Transcript: Human NM_000791.4

Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
DHFR (1719)
Length:
3919
CDS:
495..1058

Additional Resources:

NCBI RefSeq record:
NM_000791.4
NBCI Gene record:
DHFR (1719)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000791.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413013 GACATTCTTGCTATAACTAAG pLKO_005 1220 3UTR 100% 10.800 7.560 N DHFR n/a
2 TRCN0000039003 CTTTCCAGAAGTCTAGATGAT pLKO.1 762 CDS 100% 4.950 3.465 N DHFR n/a
3 TRCN0000039001 CCAGGCCATCTTAAACTATTT pLKO.1 879 CDS 100% 13.200 7.920 N DHFR n/a
4 TRCN0000433030 ACCACAACCTCTTCAGTAGAA pLKO_005 609 CDS 100% 4.950 2.970 N DHFR n/a
5 TRCN0000039002 CAAATAAAGTAGACATGGTCT pLKO.1 814 CDS 100% 2.640 1.584 N DHFR n/a
6 TRCN0000422184 ACCTCCACAAGGAGCTCATTT pLKO_005 740 CDS 100% 13.200 6.600 Y DHFR n/a
7 TRCN0000420418 AGTTGGTGGCAGTTCTGTTTA pLKO_005 839 CDS 100% 13.200 6.600 Y DHFR n/a
8 TRCN0000421979 GAAAGGCATTAAGTACAAATT pLKO_005 1013 CDS 100% 13.200 6.600 Y DHFR n/a
9 TRCN0000427080 GTAAACAGAATCTGGTGATTA pLKO_005 631 CDS 100% 13.200 6.600 Y DHFR n/a
10 TRCN0000039000 CCTGAGAAGAATCGACCTTTA pLKO.1 678 CDS 100% 10.800 5.400 Y DHFR n/a
11 TRCN0000161799 CCTGAGAAGAATCGACCTTTA pLKO.1 678 CDS 100% 10.800 5.400 Y DHFR2 n/a
12 TRCN0000038999 CCAGAAATTGATTTGGAGAAA pLKO.1 942 CDS 100% 4.950 2.475 Y DHFR n/a
13 TRCN0000442089 GAACATGGGCATCGGCAAGAA pLKO_005 533 CDS 100% 4.950 2.475 Y DHFR n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2181 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2181 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000791.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00443 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00443 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478285 GCAGTACTGAGTGTACAAGCGTAG pLX_317 55.7% 100% 100% V5 n/a
4 ccsbBroadEn_09809 pDONR223 100% 94.6% 91.9% None (many diffs) n/a
5 ccsbBroad304_09809 pLX_304 0% 94.6% 91.9% V5 (many diffs) n/a
6 TRCN0000478413 TATGTCCAACATCTCACTTAAACT pLX_317 66.6% 94.6% 91.9% V5 (many diffs) n/a
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