Construct: ORF ccsbBroad304_09958

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016133.1_s304c1, BRDN0000411253
Derived from:
ccsbBroadEn_09958
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZADH2 (284273)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09958
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 284273 ZADH2 zinc binding alcohol dehydr... NM_175907.6 99.9% 100% 225C>T
2 human 284273 ZADH2 zinc binding alcohol dehydr... NM_001306093.1 67.3% 67.3% 0_1ins369
3 human 284273 ZADH2 zinc binding alcohol dehydr... XM_024451166.1 67.3% 67.3% 0_1ins369
4 mouse 225791 Zadh2 zinc binding alcohol dehydr... NM_146090.5 84.5% 88% (many diffs)
5 mouse 225791 Zadh2 zinc binding alcohol dehydr... XM_006526460.3 54.8% 57.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1197
ORF length:
1131
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gcggctggtg cccaccgggg cccgggccat cgtggacatg tcgtacgccc
121gccacttcct ggacttccag ggctccgcca ttccccaagc catgcagaag ctggtggtga
181cccggctgag ccccaacttc cgcgaggccg tcaccctgag ccgggactgc ccggtgccgc
241tccccgggga cggagacctc ctcgtccgga accgatttgt tggtgttaat gcatctgaca
301tcaactattc agcaggccgc tatgacccct cagttaagcc tccctttgac ataggtttcg
361aaggcattgg ggaggtggtg gccctaggcc tctctgctag tgccagatac acagttggcc
421aagctgtggc ttacatggca cctggttctt ttgctgagta cacagttgtg cctgccagca
481ttgcaactcc agtgccctca gtgaaacccg agtatcttac cctgctggta agtggcacca
541ccgcatacat cagcctgaaa gagctcggag gactgtcgga agggaaaaaa gttttggtga
601cagcagcagc tgggggaacg ggccagtttg ccatgcagct ttcaaagaag gcaaagtgcc
661atgtaattgg aacctgctct tctgatgaaa agtctgcttt tctgaaatct cttggctgtg
721atcgtcctat caactataaa actgaacccg taggtaccgt ccttaagcag gagtaccctg
781aaggtgtcga tgtggtctat gaatctgttg ggggagccat gtttgacttg gctgtagacg
841ccctggctac gaaagggcgc ttgatagtaa tagggtttat ctctggctac caaactccta
901ctggcctttc gcctgtgaaa gcaggaacat tgccagccaa actgctcaag aaatctgcca
961gcgtacaggg cttcttcctg aaccattacc tttctaagta tcaagcagcc atgagccact
1021tgctcgagat gtgtgtgagc ggagacctgg tttgtgaggt ggaccttgga gatctgtctc
1081cagagggcag gtttactggc ctggagtcca tattccgtgc tgtcaattat atgtacatgg
1141gaaaaaacac tggaaaaatt gtagttgaat tacctcactc tgtcaacagt aagctgtacc
1201caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1261ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1321tta

Download FASTA (ORF) (Full)