Construct: ORF ccsbBroad304_10408
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008950.1_s304c1, BRDN0000404556
- Derived from:
- ccsbBroadEn_10408
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PABPC1P2 (728773)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10408
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26986 | PABPC1 | poly(A) binding protein cyt... | NM_002568.4 | 37.9% | 32.7% | (many diffs) |
| 2 | human | 26986 | PABPC1 | poly(A) binding protein cyt... | XM_005250861.3 | 37.9% | 32.7% | (many diffs) |
| 3 | human | 5042 | PABPC3 | poly(A) binding protein cyt... | NM_030979.2 | 36.4% | 30.1% | (many diffs) |
| 4 | human | 728773 | PABPC1P2 | poly(A) binding protein cyt... | NR_026904.1 | 20.5% | 1_916del;1724_3934del | |
| 5 | mouse | 18458 | Pabpc1 | poly(A) binding protein, cy... | NM_008774.3 | 36.7% | 32.7% | (many diffs) |
| 6 | mouse | 18458 | Pabpc1 | poly(A) binding protein, cy... | XM_011245339.2 | 36.7% | 32.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tgggatgctc ctgaatggtc tcaatgtatt tgttggacgg tttaggtctc 121 ctaaagaaca agaagcagaa cttggagcta gggcaaaaga gtccaaagtt ggacctgcct 181 caagtgtaaa agtaataact gatgaaggtg gaaaatccaa aggatttgga tttgtaagct 241 ttgaaaggca tgaagatcca cagacagcta tggatgtgaa tggaaaggag ttcagtggaa 301 atcaaactta catttgtcta gctccgaaaa aagtggaaga gcagacagaa cttaagtgca 361 aatttgaaca gatgaagcaa gatagaatca ccagatacca gggtgctaac gtttgtgtga 421 aaaatcttga tgagagtatt gatgatgaac gtctccagaa agaattttct ccatttggta 481 taatcaatag tgcaaatgtt atgatggagg gtggttgcag caaaaggttt ggctttgtat 541 gtttctcctc cccagaagaa gtagctaaag cagttacagc aatgaacggt aaaattgtgg 601 ccagcaagcc attgcatgta gctttagctc agtgcaaaga acagcgcctg gctcacttca 661 ctaaccaata catgcagaag atggcaagtg tacgagctgt gccccaccct gtaatcaacc 721 cctaccagcc agcaccttct acatgttact tcgtagcagc tatcccacag acccagaacc 781 gtgctgcata ctatcctcct ggccaaattg ctcaactaag accaagtcct ccctgggctg 841 ttcagagtgt cagaccttat ccattccaaa tatgcccaac tttcttgtac aaagtggttg 901 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 961 accggtggcg cgttaagtcg acaatcaacc tctggatta