Construct: ORF ccsbBroad304_10434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005205.1_s304c1, BRDN0000405616
- Derived from:
- ccsbBroadEn_10434
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IVD (3712)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10434
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_002225.5 | 99.9% | 99.7% | 30G>T |
| 2 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_001354599.3 | 93.5% | 93.3% | 30G>T;235_321del |
| 3 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_001354597.3 | 93.1% | 90.7% | (many diffs) |
| 4 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_001159508.3 | 92.8% | 92.6% | 30G>T;144_145ins90 |
| 5 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_001354598.3 | 90.6% | 84.4% | (many diffs) |
| 6 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022154.2 | 87.1% | 84.9% | (many diffs) |
| 7 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022153.1 | 86.7% | 82.4% | (many diffs) |
| 8 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_001354600.3 | 85.1% | 79.3% | (many diffs) |
| 9 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022155.2 | 84.4% | 83.2% | (many diffs) |
| 10 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022150.1 | 84.4% | 77.7% | (many diffs) |
| 11 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NM_001354601.3 | 81% | 78% | (many diffs) |
| 12 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_006720495.3 | 79.5% | 73% | (many diffs) |
| 13 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022149.1 | 76.1% | 73.2% | (many diffs) |
| 14 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022157.1 | 74.5% | 68.5% | (many diffs) |
| 15 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XR_001751264.1 | 63.1% | (many diffs) | |
| 16 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XM_017022158.2 | 59.9% | 58.2% | (many diffs) |
| 17 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | NR_148925.2 | 49.5% | (many diffs) | |
| 18 | human | 3712 | IVD | isovaleryl-CoA dehydrogenase | XR_001751263.1 | 26.7% | (many diffs) | |
| 19 | mouse | 56357 | Ivd | isovaleryl coenzyme A dehyd... | NM_019826.3 | 86.9% | 89.4% | (many diffs) |
| 20 | mouse | 56357 | Ivd | isovaleryl coenzyme A dehyd... | XM_006499925.1 | 79.9% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgagatggc gactgcgact cggctgctgg ggtgtcgtgt ggcgagctgg aggctgcggc 121 cgccgcttgc cggcttcgtt tcccagcggg cccactcgct tttgcccgtg gacgatgcaa 181 tcaatgggct aagcgaggag cagaggcagc ttcgtcagac catggctaag ttccttcagg 241 agcacctggc ccccaaggcc caggagatcg atcgcagcaa tgagttcaag aacctgcgag 301 aattttggaa gcagctgggg aacctgggcg tattgggcat cacagcccct gttcagtatg 361 gcggctccgg cctgggctac ctggagcatg tgctggtgat ggaggagata tcccgagctt 421 ccggagcagt ggggctcagt tacggtgccc actccaacct ctgcatcaac cagcttgtac 481 gcaatgggaa tgaggcccag aaagagaagt atctcccgaa gctgatcagt ggtgagtaca 541 tcggagccct ggccatgagt gagcccaatg caggctctga tgttgtctct atgaagctca 601 aagcggaaaa gaaaggaaat cactacatcc tgaatggcaa caagttctgg atcactaatg 661 gccctgatgc tgacgtcctg attgtctatg ccaagacaga tctggctgct gtgccagctt 721 ctcggggcat cacagccttc attgtggaga agggtatgcc tggctttagc acctctaaga 781 agctggacaa gctggggatg aggggctcta acacctgtga gctaatcttt gaagactgca 841 agattcctgc tgccaacatc ctgggccatg agaataaggg tgtctacgtg ctgatgagtg 901 ggctggacct ggagcggctg gtgctggccg gggggcctct tgggctcatg caagcggtcc 961 tggaccacac cattccctac ctgcacgtga gggaagcctt tggccagaag atcggccact 1021 tccagttgat gcaggggaag atggctgaca tgtacacccg cctcatggcg tgtcggcagt 1081 atgtctacaa tgtcgccaag gcctgcgatg agggccattg cactgctaag gactgtgcag 1141 gtgtgattct ttactcagct gagtgtgcca cacaggtagc cctggacggc attcagtgtt 1201 ttggtggcaa tggctacatc aatgactttc ccatgggccg ctttcttcga gatgccaagc 1261 tgtatgagat aggggctggg accagcgagg tgaggcggct ggtcatcggc agagccttca 1321 atgcagactt tcactagtca actttcttgt acaaagtggt tggtaagcct atccctaacc 1381 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1441 cgacaatcaa cctctggatt a