Transcript: Human XM_017022154.2

PREDICTED: Homo sapiens isovaleryl-CoA dehydrogenase (IVD), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
IVD (3712)
Length:
4583
CDS:
228..1538

Additional Resources:

NCBI RefSeq record:
XM_017022154.2
NBCI Gene record:
IVD (3712)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022154.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000330975 GTCGGCAGTATGTCTACAATG pLKO_005 1273 CDS 100% 10.800 15.120 N IVD n/a
2 TRCN0000331036 TCTAACACCTGTGAGCTAATC pLKO_005 1008 CDS 100% 10.800 7.560 N IVD n/a
3 TRCN0000026588 CAGGCTCTGATGTTGTCTCTA pLKO.1 772 CDS 100% 4.950 3.465 N IVD n/a
4 TRCN0000026587 CACAGCCTTCATTGTGGAGAA pLKO.1 932 CDS 100% 4.050 2.835 N IVD n/a
5 TRCN0000331034 CACAGCCTTCATTGTGGAGAA pLKO_005 932 CDS 100% 4.050 2.835 N IVD n/a
6 TRCN0000330976 GACTCAGCAGGAAGCATATTG pLKO_005 1759 3UTR 100% 13.200 7.920 N IVD n/a
7 TRCN0000026575 GATGCCAAGCTGTATGAGATA pLKO.1 1452 CDS 100% 4.950 2.970 N IVD n/a
8 TRCN0000331035 GATGCCAAGCTGTATGAGATA pLKO_005 1452 CDS 100% 4.950 2.970 N IVD n/a
9 TRCN0000256749 CGTCTGTAATCCCAGCTACTC pLKO_005 2658 3UTR 100% 4.050 2.025 Y SMIM11A n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2524 3UTR 100% 13.200 6.600 Y LIAS n/a
11 TRCN0000026505 GAGTTCAAGAACCTGCGAGAA pLKO.1 396 CDS 100% 4.050 2.835 N IVD n/a
12 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 3283 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022154.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10434 pDONR223 98.9% 87.1% 84.9% None (many diffs) n/a
2 ccsbBroad304_10434 pLX_304 0% 87.1% 84.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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