Construct: ORF ccsbBroad304_10588

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017472.1_s304c1, BRDN0000462190
Derived from:
ccsbBroadEn_10588
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIPSL (266971)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10588
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 266971 PIPSL PIP5K1A and PSMD4 like (pse... NR_002319.2 68.4% (many diffs)
2 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_024450131.1 56.3% 54.3% (many diffs)
3 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_006711567.4 55.5% 53.6% (many diffs)
4 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_011510048.3 55.5% 53.6% (many diffs)
5 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_011510049.3 55.5% 53.6% (many diffs)
6 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_017002441.2 55.5% 53.6% (many diffs)
7 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_011510046.3 54.6% 52.7% (many diffs)
8 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_005245532.4 54.6% 52.6% (many diffs)
9 human 8394 PIP5K1A phosphatidylinositol-4-phos... NM_003557.3 54.1% 52.2% (many diffs)
10 human 8394 PIP5K1A phosphatidylinositol-4-phos... NM_001330689.2 54.1% 52.1% (many diffs)
11 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_011510045.3 53.9% 52% (many diffs)
12 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_005245530.5 53.8% 51.9% (many diffs)
13 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_024450130.1 53.8% 51.9% (many diffs)
14 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_011510043.3 53.8% 51.9% (many diffs)
15 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_005245528.5 53.5% 51.6% (many diffs)
16 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_005245527.4 53.4% 51.5% (many diffs)
17 human 8394 PIP5K1A phosphatidylinositol-4-phos... NM_001135638.2 53.4% 51.5% (many diffs)
18 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_006711564.4 53.4% 51.5% (many diffs)
19 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_024450129.1 53.1% 51.2% (many diffs)
20 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_006711568.4 53.1% 51.2% (many diffs)
21 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_005245525.5 52.8% 50.9% (many diffs)
22 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_006711563.4 52.7% 50.8% (many diffs)
23 human 8394 PIP5K1A phosphatidylinositol-4-phos... NM_001135636.2 51% 49% (many diffs)
24 human 8394 PIP5K1A phosphatidylinositol-4-phos... NM_001135637.2 48.9% 47.3% (many diffs)
25 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_017002440.1 45.7% 44.4% (many diffs)
26 human 8394 PIP5K1A phosphatidylinositol-4-phos... XM_017002439.1 45.7% 44.4% (many diffs)
27 human 5710 PSMD4 proteasome 26S subunit, non... NM_002810.3 42.1% 40.1% (many diffs)
28 human 5710 PSMD4 proteasome 26S subunit, non... NM_001330692.2 41.9% 40% (many diffs)
29 mouse 18720 Pip5k1a phosphatidylinositol-4-phos... NM_008847.3 48.2% 48.4% (many diffs)
30 mouse 18720 Pip5k1a phosphatidylinositol-4-phos... NM_001293707.1 48.1% 48.3% (many diffs)
31 mouse 18720 Pip5k1a phosphatidylinositol-4-phos... XM_017319485.1 43.5% 44.3% (many diffs)
32 mouse 19185 Psmd4 proteasome (prosome, macrop... NM_008951.2 38.2% 38.3% (many diffs)
33 mouse 19185 Psmd4 proteasome (prosome, macrop... NM_001282017.1 38.1% 38.2% (many diffs)
34 mouse 19185 Psmd4 proteasome (prosome, macrop... XM_006501137.2 27.4% 27.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2655
ORF length:
2586
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcatctgag gtgccttatg cctctggcat gcccatcaag aaaataggcc
121atagaagtgt tgattcctca ggagggacaa cctcatcagc cttgaaaggt gccatccagt
181taggcattac ccacactgtg gggagcctga gtaccaaacc agagagtgat gtcctcatgc
241aagatttcca catggtggag agtatcttct ttcccagtga agggagcaac ctgacccctg
301ctcatcacta caatgccttt cgtttcaaga cctatgcacc tgttgccttc cgctacttct
361gggagctatt tggtatccgg cccgatgatt acttgtattc cctctgcagt gagccgctga
421ttgaactctg tagctctgga gctagtggtt ccctgttcta tgtgtccagc gacgatgagt
481tcattgttaa gacagtccga cataaagaag cggagtttct gcagaagctg cttccaggat
541actacataaa cctcaaccag aaccctcgga ctttgctgcc taaattctat ggactgtact
601gtgtgcagac aggtggcaag aacattcgga ttgtggtgat gaacaatctt ttaccaagat
661cggtaaaaat gcatatcaaa tatgacctca aaggctcaac ctacaggcgg cgggcttccc
721agaaagagcg agagaagcct cttcccacat ttaaagacct agacttctta caagacatcc
781ctgatggtct ttttttggat gctgacgtgc acaacgctct ctgtaagacc ctgcagcgtg
841actgtttggt gctgcagagc ttcaagataa tggattatag cctcttgatg tcaatccata
901atatagatca tgcacaacga gagcccttaa gcagcgaaac acaatactca gttgatactc
961gaagaccagc cccccaaaag gctctgtatt ccacagccat ggaatccatc cagggagagg
1021ctcgacgggg tggcaccatg gagaccgatg accatatggg tggcatccct gcccggaata
1081gtaaagggga aaggcttctg ctttatattg gcatcattga cattctacag tcttacaggt
1141ttgttaagaa gttggagcac tcttggaaag ccctgataca tgacggagac actgtctcag
1201tgcatcgccc aggcttctat gctgaatggt tccagcgctt catgtgcaac acggtattta
1261agaagattcc cttgaagcct tctccttcca aaaaacttcg gtctggctca tctttctctc
1321agcgagcagg ctccagtggc aactcctgca ttacttacaa gccattggtc tctggggaac
1381acaaggcaca agtgacaaca aaggcggaag tggagccagg tgttcacctt ggttgtcctg
1441atgttttacc tcagactcca cctttggagg aaatcagtga gggctcacct actcctgacc
1501ccagtttctc acctctagtt gaagagactt tgcaaatgct aactacaagc gtggacaaca
1561gtgagtatat ggggaatgga gacttcttac ccacccggct gcaggcccag caggatgctg
1621tcaacacagt ttgtcattca aagacccgca gcaaccctga gaacaacgtg ggccttatca
1681cactggataa tgactgtgaa gtgctgacca cactcacccc agacactggc cgtatcctgt
1741ccaagctaca tactgtccaa cccaagggca agatcacctt ctgcatgggc atccacgtgg
1801cccatctggc tctgaagcac cgacaaggca acaatcacaa gatccgcatc attgcctttg
1861tgggaaaccc ggtggaggac aatgagaaga atctggtgaa actggctaaa tgcctcaaga
1921aggagaaagt aaatgttgac attatcaatt ttggggaaga ggaggtgaac acagaaaagc
1981tgacagcctt tgtaaacacg ttgaatggca aagatggaac cggttctcat ctggtgacag
2041tgcctcctgg gcccagtttg gctgatgctc tcatcagttt tccgattttg gctggtgaag
2101gtggtgccat gatgggtctt ggtgccagtg actttgaatt tggagtagat cccagtgctg
2161atcctgagct ggccttggtc cttcgtgtat ttatggaaga gcagcggcag cggcaggagg
2221aggaggcccg gcaggcagct gcagcttctg ctgctgaggc cgggattgct acgactggga
2281ctgaagactc agacgatgcc ctgctgaaga tgaccatcag ccagcaagag tttggccaca
2341ctgggcttcc tgacctaagc agtatgactg aggaagagaa gattgtttgt gccatgcaga
2401tgtccctgca gggagcagag tttggcctgg cagaatcagc agacattgat gccagctcag
2461ccatggacac atctgagcca gccaaggagg aggatgatta cgacgtgatg caagaccctg
2521agttccttca gagtgtccta gagaacctcc caggtgtgga tcccaacaat gaagccattc
2581gaaatgctgt gggctccctg gcctcccagg ccaccaagga cagcaagaag gacaagaagg
2641aggaagacga gaagttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
2701ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2761cgacaatcaa cctctggatt a

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