Construct: ORF ccsbBroad304_10777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007777.1_s304c1, BRDN0000402578
- Derived from:
- ccsbBroadEn_10777
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGUOK (1716)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10777
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318862.2 | 52.2% | 50.5% | (many diffs) |
| 2 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318863.2 | 52.2% | 50.5% | (many diffs) |
| 3 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318860.2 | 51.1% | 51.1% | 153_416del |
| 4 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318861.2 | 51.1% | 51.1% | 153_416del |
| 5 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_024452739.1 | 51.1% | 51.1% | 153_416del |
| 6 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080918.3 | 48.6% | 48.6% | 1_291del |
| 7 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_001318859.2 | 45.5% | 45.5% | 1_291del;423_424insAGGTCTGTGTACAGTGAC |
| 8 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134893.2 | 39.5% | 1_209del;486_698del | |
| 9 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134896.2 | 36.9% | 1_209del;341_342insAGGTCTGTGTACAGTGAC;468_680del | |
| 10 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134898.2 | 33.9% | 1_209del;362_477del;602_814del | |
| 11 | human | 1716 | DGUOK | deoxyguanosine kinase | XM_011532647.2 | 33.5% | 32.4% | (many diffs) |
| 12 | human | 1716 | DGUOK | deoxyguanosine kinase | NM_080916.3 | 33.2% | 33.2% | 1_291del;444_707del |
| 13 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134894.2 | 32.6% | 1_209del;359_506del;634_846del | |
| 14 | human | 1716 | DGUOK | deoxyguanosine kinase | NR_134897.2 | 31% | 1_295del;445_550del;678_890del | |
| 15 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_001738656.1 | 27.7% | 1_375del;528_643del;768_995del | |
| 16 | human | 1716 | DGUOK | deoxyguanosine kinase | XR_244926.3 | 26.9% | 1_374del;524_671del;799_1026del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 342
- ORF length:
- 276
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat gtaccgggag ccagcacgat ggtcctacac attccagaca ttttcctttt 121 tgagccgcct gaaagtacag ctggagccct tccctgagaa actcttacag gccaggaagc 181 cagtacagat ctttgagagg tctgtgtaca gtgacaggct ccactttgag gctctgatga 241 acattccagt gctggtgttg gatgtcaatg atgatttttc tgaggaagta accaaacaag 301 aagacctcat gagagaggta aacacctttg taaagaatct gtacccaact ttcttgtaca 361 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 421 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta