Transcript: Human XM_011532647.2

PREDICTED: Homo sapiens deoxyguanosine kinase (DGUOK), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DGUOK (1716)
Length:
1122
CDS:
82..897

Additional Resources:

NCBI RefSeq record:
XM_011532647.2
NBCI Gene record:
DGUOK (1716)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147942 CTACAGAACCTGTAGCAACA pXPR_003 TGG 219 27% 2 0.6329 DGUOK DGUOK 76603
2 BRDN0001147023 AGGAGGAAACGCCCTCGAGT pXPR_003 GGG 68 8% 1 0.3622 DGUOK DGUOK 76602
3 BRDN0001148589 GCTATTTGCATTGCAGCTGT pXPR_003 GGG 143 18% 2 -0.1930 DGUOK DGUOK 76604
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011532647.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380303 CAGTGACATCGAGTGGCATAT pLKO_005 546 CDS 100% 10.800 15.120 N DGUOK n/a
2 TRCN0000010993 CACTGCCCAAAGTCTTGGAAA pLKO.1 342 CDS 100% 4.950 6.930 N DGUOK n/a
3 TRCN0000197112 GACCTCATGAGAGAGGTAAAC pLKO.1 856 CDS 100% 1.080 1.512 N DGUOK n/a
4 TRCN0000219739 TTTGCCAGCCGGATCACATTA pLKO.1 601 CDS 100% 13.200 10.560 N DGUOK n/a
5 TRCN0000219738 ACATCGAGTGGCATATCTATC pLKO.1 551 CDS 100% 10.800 8.640 N DGUOK n/a
6 TRCN0000006077 AGCACGATGGTCCTACACATT pLKO.1 390 CDS 100% 4.950 3.960 N DGUOK n/a
7 TRCN0000338412 AGCACGATGGTCCTACACATT pLKO_005 390 CDS 100% 4.950 3.960 N DGUOK n/a
8 TRCN0000382383 CAACACGAAGCCTGGCTTATT pLKO_005 736 CDS 100% 13.200 9.240 N DGUOK n/a
9 TRCN0000006075 TCCTACACATTCCAGACATTT pLKO.1 400 CDS 100% 13.200 9.240 N DGUOK n/a
10 TRCN0000006076 CTCTCCATCGAAGGCAACATT pLKO.1 202 CDS 100% 5.625 3.938 N DGUOK n/a
11 TRCN0000338411 CTCTCCATCGAAGGCAACATT pLKO_005 202 CDS 100% 5.625 3.938 N DGUOK n/a
12 TRCN0000006074 CCTGACTTTCTGAAGCTAGAA pLKO.1 933 3UTR 100% 4.950 3.465 N DGUOK n/a
13 TRCN0000338413 CCTGACTTTCTGAAGCTAGAA pLKO_005 933 3UTR 100% 4.950 3.465 N DGUOK n/a
14 TRCN0000195678 CCACGTTTGTGAAGTTACTCA pLKO.1 236 CDS 100% 3.000 2.100 N DGUOK n/a
15 TRCN0000338472 CCACGTTTGTGAAGTTACTCA pLKO_005 236 CDS 100% 3.000 2.100 N DGUOK n/a
16 TRCN0000195404 CCAATACCATGAAGTTCAGGC pLKO.1 898 CDS 100% 2.160 1.512 N DGUOK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011532647.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00441 pDONR223 100% 97.8% 97.8% None 423_424insAGGTCTGTGTACAGTGAC n/a
2 ccsbBroad304_00441 pLX_304 0% 97.8% 97.8% V5 423_424insAGGTCTGTGTACAGTGAC n/a
3 TRCN0000480725 AAAACGCGAACATGTACCTCGCAC pLX_317 53.1% 97.8% 97.8% V5 423_424insAGGTCTGTGTACAGTGAC n/a
4 ccsbBroadEn_14614 pDONR223 0% 97.8% 97.8% None 423_424insAGGTCTGTGTACAGTGAC n/a
5 ccsbBroad304_14614 pLX_304 0% 97.8% 97.8% V5 423_424insAGGTCTGTGTACAGTGAC n/a
6 TRCN0000471786 AACATGAGCGCGCTTACCGAGTAA pLX_317 52.3% 97.8% 97.8% V5 (not translated due to prior stop codon) 423_424insAGGTCTGTGTACAGTGAC n/a
7 TRCN0000491324 GCTAAGGACGCAATCATAGTCAAC pLX_317 42.9% 97.8% 97.8% V5 (not translated due to prior stop codon) 423_424insAGGTCTGTGTACAGTGAC n/a
8 TRCN0000489258 GTCTGGCTTAAGCCTTTACTATTC pLX_317 42.1% 97.7% 97.4% V5 423_424insAGGTCTGTGTACAGTGAC;813_814insG n/a
9 ccsbBroadEn_10777 pDONR223 100% 33.5% 32.4% None (many diffs) n/a
10 ccsbBroad304_10777 pLX_304 0% 33.5% 32.4% V5 (many diffs) n/a
11 TRCN0000471348 CAATAATAGTGCTCTGAGAGCACT pLX_317 100% 33.5% 32.4% V5 (many diffs) n/a
12 TRCN0000487744 TTCGACTTTACGATACGGTGATCC pLX_317 67.5% 32.8% 18.6% V5 (not translated due to prior stop codon) 143_255del;381_813del n/a
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