Transcript: Human NR_134896.2

Homo sapiens deoxyguanosine kinase (DGUOK), transcript variant 11, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
DGUOK (1716)
Length:
680
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_134896.2
NBCI Gene record:
DGUOK (1716)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_134896.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010993 CACTGCCCAAAGTCTTGGAAA pLKO.1 179 3UTR 100% 4.950 6.930 N DGUOK n/a
2 TRCN0000197112 GACCTCATGAGAGAGGTAAAC pLKO.1 429 3UTR 100% 1.080 1.512 N DGUOK n/a
3 TRCN0000006077 AGCACGATGGTCCTACACATT pLKO.1 227 3UTR 100% 4.950 3.960 N DGUOK n/a
4 TRCN0000338412 AGCACGATGGTCCTACACATT pLKO_005 227 3UTR 100% 4.950 3.960 N DGUOK n/a
5 TRCN0000006075 TCCTACACATTCCAGACATTT pLKO.1 237 3UTR 100% 13.200 9.240 N DGUOK n/a
6 TRCN0000006076 CTCTCCATCGAAGGCAACATT pLKO.1 152 3UTR 100% 5.625 3.938 N DGUOK n/a
7 TRCN0000338411 CTCTCCATCGAAGGCAACATT pLKO_005 152 3UTR 100% 5.625 3.938 N DGUOK n/a
8 TRCN0000006074 CCTGACTTTCTGAAGCTAGAA pLKO.1 506 3UTR 100% 4.950 3.465 N DGUOK n/a
9 TRCN0000338413 CCTGACTTTCTGAAGCTAGAA pLKO_005 506 3UTR 100% 4.950 3.465 N DGUOK n/a
10 TRCN0000195404 CCAATACCATGAAGTTCAGGC pLKO.1 471 3UTR 100% 2.160 1.512 N DGUOK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_134896.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00441 pDONR223 100% 40.5% None (many diffs) n/a
2 ccsbBroad304_00441 pLX_304 0% 40.5% V5 (many diffs) n/a
3 TRCN0000480725 AAAACGCGAACATGTACCTCGCAC pLX_317 53.1% 40.5% V5 (many diffs) n/a
4 ccsbBroadEn_14614 pDONR223 0% 40.5% None (many diffs) n/a
5 ccsbBroad304_14614 pLX_304 0% 40.5% V5 (many diffs) n/a
6 TRCN0000471786 AACATGAGCGCGCTTACCGAGTAA pLX_317 52.3% 40.5% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489258 GTCTGGCTTAAGCCTTTACTATTC pLX_317 42.1% 40.5% V5 (many diffs) n/a
8 TRCN0000491324 GCTAAGGACGCAATCATAGTCAAC pLX_317 42.9% 40.5% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000487744 TTCGACTTTACGATACGGTGATCC pLX_317 67.5% 39.2% V5 (not translated due to prior stop codon) 1_31del;299_680del n/a
10 ccsbBroadEn_10777 pDONR223 100% 36.9% None 1_209del;341_342insAGGTCTGTGTACAGTGAC;468_680del n/a
11 ccsbBroad304_10777 pLX_304 0% 36.9% V5 1_209del;341_342insAGGTCTGTGTACAGTGAC;468_680del n/a
12 TRCN0000471348 CAATAATAGTGCTCTGAGAGCACT pLX_317 100% 36.9% V5 1_209del;341_342insAGGTCTGTGTACAGTGAC;468_680del n/a
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