Transcript: Human NM_001318861.2

Homo sapiens deoxyguanosine kinase (DGUOK), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
DGUOK (1716)
Length:
1048
CDS:
296..838

Additional Resources:

NCBI RefSeq record:
NM_001318861.2
NBCI Gene record:
DGUOK (1716)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318861.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380303 CAGTGACATCGAGTGGCATAT pLKO_005 487 CDS 100% 10.800 15.120 N DGUOK n/a
2 TRCN0000010993 CACTGCCCAAAGTCTTGGAAA pLKO.1 265 5UTR 100% 4.950 6.930 N DGUOK n/a
3 TRCN0000197112 GACCTCATGAGAGAGGTAAAC pLKO.1 797 CDS 100% 1.080 1.512 N DGUOK n/a
4 TRCN0000219739 TTTGCCAGCCGGATCACATTA pLKO.1 542 CDS 100% 13.200 10.560 N DGUOK n/a
5 TRCN0000219738 ACATCGAGTGGCATATCTATC pLKO.1 492 CDS 100% 10.800 8.640 N DGUOK n/a
6 TRCN0000006077 AGCACGATGGTCCTACACATT pLKO.1 313 CDS 100% 4.950 3.960 N DGUOK n/a
7 TRCN0000338412 AGCACGATGGTCCTACACATT pLKO_005 313 CDS 100% 4.950 3.960 N DGUOK n/a
8 TRCN0000382383 CAACACGAAGCCTGGCTTATT pLKO_005 677 CDS 100% 13.200 9.240 N DGUOK n/a
9 TRCN0000006075 TCCTACACATTCCAGACATTT pLKO.1 323 CDS 100% 13.200 9.240 N DGUOK n/a
10 TRCN0000006076 CTCTCCATCGAAGGCAACATT pLKO.1 152 5UTR 100% 5.625 3.938 N DGUOK n/a
11 TRCN0000338411 CTCTCCATCGAAGGCAACATT pLKO_005 152 5UTR 100% 5.625 3.938 N DGUOK n/a
12 TRCN0000006074 CCTGACTTTCTGAAGCTAGAA pLKO.1 874 3UTR 100% 4.950 3.465 N DGUOK n/a
13 TRCN0000338413 CCTGACTTTCTGAAGCTAGAA pLKO_005 874 3UTR 100% 4.950 3.465 N DGUOK n/a
14 TRCN0000195404 CCAATACCATGAAGTTCAGGC pLKO.1 839 CDS 100% 2.160 1.512 N DGUOK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318861.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00441 pDONR223 100% 64.9% 64.9% None 0_1ins291 n/a
2 ccsbBroad304_00441 pLX_304 0% 64.9% 64.9% V5 0_1ins291 n/a
3 TRCN0000480725 AAAACGCGAACATGTACCTCGCAC pLX_317 53.1% 64.9% 64.9% V5 0_1ins291 n/a
4 ccsbBroadEn_14614 pDONR223 0% 64.9% 64.9% None 0_1ins291 n/a
5 ccsbBroad304_14614 pLX_304 0% 64.9% 64.9% V5 0_1ins291 n/a
6 TRCN0000471786 AACATGAGCGCGCTTACCGAGTAA pLX_317 52.3% 64.9% 64.9% V5 (not translated due to prior stop codon) 0_1ins291 n/a
7 TRCN0000491324 GCTAAGGACGCAATCATAGTCAAC pLX_317 42.9% 64.9% 64.9% V5 (not translated due to prior stop codon) 0_1ins291 n/a
8 TRCN0000489258 GTCTGGCTTAAGCCTTTACTATTC pLX_317 42.1% 64.9% 64.7% V5 0_1ins291;540_541insG n/a
9 ccsbBroadEn_10777 pDONR223 100% 51.1% 51.1% None 153_416del n/a
10 ccsbBroad304_10777 pLX_304 0% 51.1% 51.1% V5 153_416del n/a
11 TRCN0000471348 CAATAATAGTGCTCTGAGAGCACT pLX_317 100% 51.1% 51.1% V5 153_416del n/a
12 TRCN0000487744 TTCGACTTTACGATACGGTGATCC pLX_317 67.5% 12.3% 12.7% V5 (not translated due to prior stop codon) 0_1ins178;90_540del n/a
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