Construct: ORF ccsbBroad304_10929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009352.1_s304c1, BRDN0000461791
- Derived from:
- ccsbBroadEn_10929
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JAK2 (3717)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10929
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322194.1 | 99.7% | 99.7% | 94_102delATGAAGCAA;2490G>A |
| 2 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322195.1 | 99.7% | 99.7% | 94_102delATGAAGCAA;2490G>A |
| 3 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322196.1 | 99.7% | 99.7% | 94_102delATGAAGCAA;2490G>A |
| 4 | human | 3717 | JAK2 | Janus kinase 2 | NM_004972.3 | 99.7% | 99.7% | 94_102delATGAAGCAA;2490G>A |
| 5 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322204.1 | 87% | 87% | 0_1ins438;2043G>A |
| 6 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322198.1 | 64.3% | 64.3% | 0_1ins1206;1275G>A |
| 7 | human | 3717 | JAK2 | Janus kinase 2 | NM_001322199.1 | 64.3% | 64.3% | 0_1ins1206;1275G>A |
| 8 | mouse | 16452 | Jak2 | Janus kinase 2 | NM_001048177.2 | 88.4% | 93.5% | (many diffs) |
| 9 | mouse | 16452 | Jak2 | Janus kinase 2 | NM_008413.3 | 88.4% | 93.5% | (many diffs) |
| 10 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_011247155.2 | 88.4% | 93.5% | (many diffs) |
| 11 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526713.3 | 84.9% | 89.6% | (many diffs) |
| 12 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526714.3 | 73.7% | 78.2% | (many diffs) |
| 13 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_017318068.1 | 57.2% | 61.3% | (many diffs) |
| 14 | mouse | 16452 | Jak2 | Janus kinase 2 | XM_006526715.3 | 46.3% | 47.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3453
- ORF length:
- 3387
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg aatggcctgc cttacgatga cagaaatgga gggaacatcc acctcttcta 121 tatatcagaa tggtgatatt tctggaaatg ccaattctat agatccagtt cttcaggtgt 181 atctttacca ttcccttggg aaatctgagg cagattatct gacctttcca tctggggagt 241 atgttgcaga agaaatctgt attgctgctt ctaaagcttg tggtatcaca cctgtgtatc 301 ataatatgtt tgctttaatg agtgaaacag aaaggatctg gtatccaccc aaccatgtct 361 tccatataga tgagtcaacc aggcataatg tactctacag aataagattt tactttcctc 421 gttggtattg cagtggcagc aacagagcct atcggcatgg aatatctcga ggtgctgaag 481 ctcctcttct tgatgacttt gtcatgtctt acctctttgc tcagtggcgg catgattttg 541 tgcacggatg gataaaagta cctgtgactc atgaaacaca ggaagaatgt cttgggatgg 601 cagtgttaga tatgatgaga atagccaaag aaaacgatca aaccccactg gccatctata 661 actctatcag ctacaagaca ttcttaccaa aatgtattcg agcaaagatc caagactatc 721 atattttgac aaggaagcga ataaggtaca gatttcgcag atttattcag caattcagcc 781 aatgcaaagc cactgccaga aacttgaaac ttaagtatct tataaatctg gaaactctgc 841 agtctgcctt ctacacagag aaatttgaag taaaagaacc tggaagtggt ccttcaggtg 901 aggagatttt tgcaaccatt ataataactg gaaacggtgg aattcagtgg tcaagaggga 961 aacataaaga aagtgagaca ctgacagaac aggatttaca gttatattgc gattttccta 1021 atattattga tgtcagtatt aagcaagcaa accaagaggg ttcaaatgaa agccgagttg 1081 taactatcca taagcaagat ggtaaaaatc tggaaattga acttagctca ttaagggaag 1141 ctttgtcttt cgtgtcatta attgatggat attatagatt aactgcagat gcacatcatt 1201 acctctgtaa agaagtagca cctccagccg tgcttgaaaa tatacaaagc aactgtcatg 1261 gcccaatttc gatggatttt gccattagta aactgaagaa agcaggtaat cagactggac 1321 tgtatgtact tcgatgcagt cctaaggact ttaataaata ttttttgact tttgctgtcg 1381 agcgagaaaa tgtcattgaa tataaacact gtttgattac aaaaaatgag aatgaagagt 1441 acaacctcag tgggacaaag aagaacttca gcagtcttaa agatcttttg aattgttacc 1501 agatggaaac tgttcgctca gacaatataa ttttccagtt tactaaatgc tgtcccccaa 1561 agccaaaaga taaatcaaac cttctagtct tcagaacgaa tggtgtttct gatgtaccaa 1621 cctcaccaac attacagagg cctactcata tgaaccaaat ggtgtttcac aaaatcagaa 1681 atgaagattt gatatttaat gaaagccttg gccaaggcac ttttacaaag atttttaaag 1741 gcgtacgaag agaagtagga gactacggtc aactgcatga aacagaagtt cttttaaaag 1801 ttctggataa agcacacaga aactattcag agtctttctt tgaagcagca agtatgatga 1861 gcaagctttc tcacaagcat ttggttttaa attatggagt atgtgtctgt ggagacgaga 1921 atattctggt tcaggagttt gtaaaatttg gatcactaga tacatatctg aaaaagaata 1981 aaaattgtat aaatatatta tggaaacttg aagttgctaa acagttggca tgggccatgc 2041 attttctaga agaaaacacc cttattcatg ggaatgtatg tgccaaaaat attctgctta 2101 tcagagaaga agacaggaag acaggaaatc ctcctttcat caaacttagt gatcctggca 2161 ttagtattac agttttgcca aaggacattc ttcaggagag aataccatgg gtaccacctg 2221 aatgcattga aaatcctaaa aatttaaatt tggcaacaga caaatggagt tttggtacca 2281 ctttgtggga aatctgcagt ggaggagata aacctctaag tgctctggat tctcaaagaa 2341 agctacaatt ttatgaagat aggcatcagc ttcctgcacc aaagtgggca gaattagcaa 2401 accttataaa taattgtatg gattatgaac cagatttcag gccttctttc agagccatca 2461 tacgagatct taacagtttg tttactccag attatgaact attaacagaa aatgacatgt 2521 taccaaatat gaggataggt gccctagggt tttctggtgc ctttgaagac cgggatccta 2581 cacagtttga agagagacat ttgaaatttc tacagcaact tggcaagggt aattttggga 2641 gtgtggagat gtgccggtat gaccctctac aggacaacac tggggaggtg gtcgctgtaa 2701 aaaagcttca gcatagtact gaagagcacc taagagactt tgaaagggaa attgaaatcc 2761 tgaaatccct acagcatgac aacattgtaa agtacaaggg agtgtgctac agtgctggtc 2821 ggcgtaatct aaaattaatt atggaatatt taccatatgg aagtttacga gactatcttc 2881 aaaaacataa agaacggata gatcacataa aacttctgca gtacacatct cagatatgca 2941 agggtatgga gtatcttggt acaaaaaggt atatccacag ggatctggca acgagaaata 3001 tattggtgga gaacgagaac agagttaaaa ttggagattt tgggttaacc aaagtcttgc 3061 cacaagacaa agaatactat aaagtaaaag aacctggtga aagtcccata ttctggtatg 3121 ctccagaatc actgacagag agcaagtttt ctgtggcctc agatgtttgg agctttggag 3181 tggttctgta tgaacttttc acatacattg agaagagtaa aagtccacca gcggaattta 3241 tgcgtatgat tggcaatgac aaacaaggac agatgatcgt gttccatttg atagaacttt 3301 tgaagaataa tggaagatta ccaagaccag atggatgccc agatgagatc tatatgatca 3361 tgacagaatg ctggaacaat aatgtaaatc aacgcccctc ctttagggat ctagctcttc 3421 gagtggatca aataagggat aacatggctg gatgcccaac tttcttgtac aaagtggttg 3481 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 3541 accggtggcg cgttaagtcg acaatcaacc tctggatta