Construct: ORF ccsbBroad304_11040

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012166.1_s304c1, BRDN0000460334
Derived from:
ccsbBroadEn_11040
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PMS2P3 (5387)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11040
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100289561 LOC100289561 uncharacterized LOC100289561 NM_001242713.3 55.5% 51.4% (many diffs)
2 human 100289561 LOC100289561 uncharacterized LOC100289561 NM_001347879.2 55.5% 51.4% (many diffs)
3 human 100289561 LOC100289561 uncharacterized LOC100289561 NM_001347880.2 55.5% 51.4% (many diffs)
4 human 100533952 RBAK-RBAKDN RBAK-RBAKDN readthrough NM_001204513.3 51.8% 40.7% (many diffs)
5 human 100101440 PMS2P7 PMS1 homolog 2, mismatch re... NR_130940.1 37.9% (many diffs)
6 human 5382 PMS2P4 PMS1 homolog 2, mismatch re... NR_022007.2 37.4% (many diffs)
7 human 5380 PMS2P2 PMS1 homolog 2, mismatch re... NR_003614.3 34.8% (many diffs)
8 human 5382 PMS2P4 PMS1 homolog 2, mismatch re... NR_046299.1 34.7% (many diffs)
9 human 5387 PMS2P3 PMS1 homolog 2, mismatch re... NR_028059.1 33.3% (many diffs)
10 human 5382 PMS2P4 PMS1 homolog 2, mismatch re... NR_046297.1 31.9% (many diffs)
11 human 5383 PMS2P5 PMS1 homolog 2, mismatch re... NR_027776.2 21.7% (many diffs)
12 human 5383 PMS2P5 PMS1 homolog 2, mismatch re... NR_027775.2 20.2% (many diffs)
13 human 5395 PMS2 PMS1 homolog 2, mismatch re... NM_001322006.2 10.2% 8.7% (many diffs)
14 human 5395 PMS2 PMS1 homolog 2, mismatch re... NM_000535.7 9.6% 8.2% (many diffs)
15 human 5395 PMS2 PMS1 homolog 2, mismatch re... NM_001322014.2 9.5% 8.1% (many diffs)
16 human 5395 PMS2 PMS1 homolog 2, mismatch re... NR_136154.1 6.2% (many diffs)
17 mouse 18861 Pms2 postmeiotic segregation inc... NM_008886.2 8.8% 7.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
576
ORF length:
507
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaacacattg caggggccag tgtcattcaa agatgtggct gtggatttca
121cccaggagga gtggcggcaa ctggaccctg atgagaagat agcatacggg gatgtgatgt
181tggagaacta cagccatcta gtttctgtgg ggtatgatta tcaccaagcc aaacatcatc
241atggagtgga ggtgaaggaa gtggagcagg gagaggagcc gtggataatg gaaggtgaat
301ttccatgtca acatagtcca gaacctgcta aggccatcaa acctattgat cggaagtcag
361tccatcagat ttgctctggg ccagtggtac tgagtctaag cactgcagtg aaggagttag
421tagaaaacag tctggatgct ggtgccacta atattgatct aaagcttaag gactatggag
481tggatctcat tgaagtttca gacaatggat gtggggtaga agaagaaaac tttgaaggct
541taacgatgtc accatttcta cctgccacgc gtcggttgcc aactttcttg tacaaagtgg
601ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
661ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)