Construct: ORF ccsbBroad304_11113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012717.1_s304c1, BRDN0000408738
- Derived from:
- ccsbBroadEn_11113
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN1 (6252)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11113
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6252 | RTN1 | reticulon 1 | NM_021136.3 | 90.3% | 90.4% | 1_222del;2106C>T;2322T>C |
| 2 | human | 6252 | RTN1 | reticulon 1 | XM_011537063.3 | 68.9% | 65.1% | (many diffs) |
| 3 | human | 6252 | RTN1 | reticulon 1 | NM_206852.2 | 28.6% | 27.5% | (many diffs) |
| 4 | human | 6252 | RTN1 | reticulon 1 | NM_001363702.1 | 27.2% | 27% | (many diffs) |
| 5 | mouse | 104001 | Rtn1 | reticulon 1 | NM_001007596.2 | 26.1% | 27.1% | (many diffs) |
| 6 | mouse | 104001 | Rtn1 | reticulon 1 | NR_104446.1 | 19.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2175
- ORF length:
- 2106
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaactgca tccacaggtg tggcaggtgt ttccagtgcc atggaccaca 121 ccttctcaac aacatcaaaa gatggggaag gatcgtgtta cacatctctc atttctgaca 181 tctgctatcc acctcaggag gattctacat attttactgg aattcttcag aaggaaaatg 241 gccacgtcac catttcagag agccctgagg agctgggtac acccggcccc tccttaccag 301 atgtgcctgg gatagagtct cgtggcttat ttagttctga ttctggaata gagatgactc 361 ctgcagagtc cacggaagtg aacaagatct tagcagaccc tctggaccag atgaaagcag 421 aggcctataa atacattgac ataaccagac ccgaggaggt gaagcaccaa gaacaacatc 481 accccgagct ggaagataaa gacttggact ttaagaataa agacactgac atctcaatta 541 aacctgaagg agtccgtgaa cctgacaaac cagctcctgt ggagggaaaa atcatcaagg 601 accatttatt ggaagaatcc acatttgctc catacataga tgatctctct gaagaacagc 661 gcagggctcc tcagatcacc acccctgtca aaatcacact gacggaaata gaaccttctg 721 ttgaaaccac tacccaagag aagacccctg agaagcaaga tatatgtcta aagccaagtc 781 ctgacacagt ccccactgtc actgtctcgg agcctgaaga cgacagccca ggatctatca 841 cccctccatc ttctggaaca gaaccatctg ctgcagaatc ccaggggaaa ggcagcatct 901 ccgaggatga gctgatcacc gccatcaaag aagcaaaggg attatcgtat gaaaccgccg 961 agaacccacg gccggtgggc cagctggccg acaggcccga ggtcaaggcc aggtccggac 1021 cgccaaccat ccccagcccc ctggaccacg aggccagcag cgcggagtcg ggggactcag 1081 agatcgagct ggtgtccgag gaccccatgg ccgcggagga cgcgctgccc tcaggctatg 1141 tgagctttgg ccacgtgggc ggcccgccgc cctcgcccgc ctcgccatcc atccagtaca 1201 gcatcctgag ggaggagcgc gaggccgagc tggacagcga gctcatcatc gagtcgtgcg 1261 acgcctcctc ggcctcggag gagagcccca agcgggagca ggactcaccc ccgatgaagc 1321 ccagcgccct ggatgccatc cgggaggaga ctggcgtccg ggccgaggag cgtgcgccaa 1381 gccggcgggg cctggccgag ccgggttcct tcctcgacta cccctcaact gagccccagc 1441 ctggccccga gctgccccct ggagacggag ccctggagcc tgagacgccc atgttgccac 1501 ggaagcctga agaagactcg agttccaacc aaagtcctgc ggccacaaag ggccctgggc 1561 ctctaggtcc tggcgccccg cccccactgc tgtttctcaa taagcaaaaa gctattgacc 1621 tgttgtattg gcgggacatc aagcagacgg gcatcgtgtt tgggagtttc ctgctgctgc 1681 tcttctccct gacccagttc agcgtggtga gcgtcgtggc ctacctggcc ctggccgcac 1741 tctcagccac catcagtttc cgcatctaca agtctgtttt acaagcagtg cagaaaaccg 1801 acgaaggcca ccctttcaag gcctacttgg agcttgagat caccctttct caggagcaga 1861 ttcagaagta cacggactgc ctgcagttct acgtgaacag cacacttaag gaactgagga 1921 ggctcttcct tgtccaggac ctggtggatt ctttaaaatt tgcagtcctg atgtggctcc 1981 tgacctacgt tggcgctctc ttcaatggcc tgaccctgct gctcatggct gtggtttcaa 2041 tgtttactct acctgtagtg tatgttaagc accaggcaca gattgaccaa tatctgggac 2101 ttgtgaggac tcacataaat gctgttgtgg caaagattca ggctaaaatc ccaggcgcta 2161 agaggcacgc tgagttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 2221 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2281 cgacaatcaa cctctggatt a