Construct: ORF ccsbBroad304_11245
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006481.1_s304c1, BRDN0000400521
- Derived from:
- ccsbBroadEn_11245
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA3B (7869)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11245
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7869 | SEMA3B | semaphorin 3B | NM_001005914.2 | 84.8% | 84.8% | 1_339del |
| 2 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290060.2 | 84.7% | 84.7% | 1_339del;990_992delCAG |
| 3 | human | 7869 | SEMA3B | semaphorin 3B | NM_004636.3 | 84.7% | 84.7% | 1_339del;990_992delCAG |
| 4 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290061.1 | 84.2% | 84% | 1_339del;923_937del;1005_1007delCAG |
| 5 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290062.1 | 63.9% | 63.9% | 0_1ins687 |
| 6 | human | 7869 | SEMA3B | semaphorin 3B | NM_001290063.1 | 63.9% | 63.9% | 0_1ins687 |
| 7 | human | 7869 | SEMA3B | semaphorin 3B | NR_110697.1 | 50.1% | (many diffs) | |
| 8 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511673.1 | 72% | 77.9% | (many diffs) |
| 9 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001042779.2 | 70.2% | 75.4% | (many diffs) |
| 10 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001291537.1 | 70.2% | 75.4% | (many diffs) |
| 11 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001291538.1 | 70.2% | 75.4% | (many diffs) |
| 12 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_001291539.1 | 70.2% | 75.4% | (many diffs) |
| 13 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | NM_009153.3 | 70.2% | 75.4% | (many diffs) |
| 14 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511664.3 | 70.2% | 75.4% | (many diffs) |
| 15 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511665.3 | 70.2% | 75.4% | (many diffs) |
| 16 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511666.3 | 70.2% | 75.4% | (many diffs) |
| 17 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511671.3 | 70.2% | 75.4% | (many diffs) |
| 18 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242862.2 | 70.2% | 75.4% | (many diffs) |
| 19 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242863.2 | 70.2% | 75.4% | (many diffs) |
| 20 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242864.2 | 70.2% | 75.4% | (many diffs) |
| 21 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242865.2 | 70.2% | 75.4% | (many diffs) |
| 22 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_011242867.2 | 70.2% | 75.4% | (many diffs) |
| 23 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_017313225.1 | 70.2% | 75.4% | (many diffs) |
| 24 | mouse | 20347 | Sema3b | sema domain, immunoglobulin... | XM_006511674.3 | 46.7% | 47.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1971
- ORF length:
- 1905
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa cttcgtgaag ttgctgcatg cctacaaccg cacccatttg ctggcctgtg 121 gcacgggagc cttccaccca acctgtgcct ttgtggaagt gggccaccgg gcagaggagc 181 ccgtcctccg gctggaccca ggaaggatag aggatggcaa ggggaagagt ccttatgacc 241 ccaggcatcg ggctgcctcc gtgctggtgg gggaggagct atactcaggg gtggcagcag 301 acctcatggg acgagacttt accatctttc gcagcctagg gcaacgtcca agtctccgaa 361 cagagccaca cgactcccgc tggctcaatg agcccaagtt tgtcaaggta ttttggatcc 421 cggagagcga gaacccagac gacgacaaaa tctacttctt ctttcgtgag acggcggtag 481 aggcggcgcc ggcactggga cgcctgtccg tgtcccgcgt tggccagatc tgccggaacg 541 acgtgggcgg ccagcgcagc ctggtcaaca agtggacgac gttcctgaag gcgcggctgg 601 tgtgctcggt gcccggcgtc gagggcgaca cccacttcga tcagctccag gatgtgtttc 661 tgttgtcctc gcgggaccac cggaccccgc tgctctatgc cgtcttctcc acgtccagca 721 tcttccaggg ctctgcggtg tgcgtgtaca gcatgaacga cgtgcgccgg gccttcttgg 781 gaccctttgc acacaaggag gggcccatgc accagtgggt gtcataccag ggtcgcgtcc 841 cctacccgcg gccaggcatg tgccccagca agacctttgg caccttcagt tccaccaagg 901 acttcccaga cgatgtcatc cagtttgcgc ggaaccaccc cctcatgtac aactctgtcc 961 tgcccactgg ggggcgccct cttttcctac aagttggagc caattacacc ttcactcaaa 1021 ttgccgcgga ccgggttgca gccgctgacg gacactatga cgtcctcttc attggcacag 1081 acgttggcac ggtgctgaag gtgatctcgg tccccaaggg cagtaggccc agcgcagagg 1141 ggctgctcct ggaggagctg cacgtgtttg aggactcggc cgctgtcacc agcatgcaaa 1201 tttcttccaa gaggcaccag ctgtacgtag cctcgcggag cgcggtggcc cagatcgcgt 1261 tgcaccgctg cgctgcccac ggccgcgtct gcaccgaatg ctgtctggcg cgtgacccct 1321 actgcgcctg ggacggggtc gcgtgcacgc gcttccagcc cagtgccaag aggcggttcc 1381 ggcggcaaga cgtaaggaat ggcgacccca gcacgttgtg ctccggagac tcgtctcgtc 1441 ccgcgctgct ggaacacaag gtgttcggcg tggagggcag cagcgccttt ctggagtgtg 1501 agccccgctc gctgcaggcg cgcgtggagt ggactttcca gcgcgcaggg gtgacagccc 1561 acacccaggt gctggcagag gagcgcaccg agcgcaccgc ccggggacta ctgctgcgca 1621 ggctgcggcg ccgggactcg ggcgtgtact tgtgcgccgc cgtcgagcag ggctttacgc 1681 aaccgctgcg tcgcctgtcg ctgcacgtgt tgagtgctac gcaggccgaa cgactggcgc 1741 gggccgagga ggctgcgccc gccgcgccgc cgggccccaa actctggtac cgggactttc 1801 tgcagctggt ggagccgggc ggaggtggca gcgcgaactc cctgcgcatg tgccgcccgc 1861 agcctgcgct gcagtcactg cccctggagt cgcggagaaa gggccgtaac cggaggaccc 1921 acgcccctga gcctcgcgct gagcgggggc cgcgcagcgc aacgcactgg tgcccaactt 1981 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2041 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta