Construct: ORF ccsbBroad304_11268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003922.1_s304c1, BRDN0000408410
- Derived from:
- ccsbBroadEn_11268
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H4C8 (8365)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11268
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8365 | H4C8 | H4 clustered histone 8 | NM_003543.3 | 98.3% | 100% | (many diffs) |
| 2 | human | 8367 | H4C5 | H4 clustered histone 5 | NM_003545.3 | 89.6% | 100% | (many diffs) |
| 3 | human | 8360 | H4C4 | H4 clustered histone 4 | NM_003539.3 | 84.4% | 100% | (many diffs) |
| 4 | human | 8362 | H4C12 | H4 clustered histone 12 | NM_003541.2 | 84.1% | 100% | (many diffs) |
| 5 | human | 8363 | H4C11 | H4 clustered histone 11 | NM_021968.3 | 84.1% | 100% | (many diffs) |
| 6 | human | 8294 | H4C9 | H4 clustered histone 9 | NM_003495.2 | 83.8% | 100% | (many diffs) |
| 7 | human | 8361 | H4C6 | H4 clustered histone 6 | NM_003540.3 | 83.8% | 100% | (many diffs) |
| 8 | human | 8366 | H4C2 | H4 clustered histone 2 | NM_003544.2 | 83.4% | 100% | (many diffs) |
| 9 | human | 554313 | H4C15 | H4 clustered histone 15 | NM_001034077.4 | 83.1% | 100% | (many diffs) |
| 10 | human | 8370 | H4C14 | H4 clustered histone 14 | NM_003548.2 | 83.1% | 100% | (many diffs) |
| 11 | human | 8359 | H4C1 | H4 clustered histone 1 | NM_003538.3 | 82.3% | 100% | (many diffs) |
| 12 | human | 8364 | H4C3 | H4 clustered histone 3 | NM_003542.3 | 81.8% | 100% | (many diffs) |
| 13 | human | 8368 | H4C13 | H4 clustered histone 13 | NM_003546.2 | 80.9% | 100% | (many diffs) |
| 14 | human | 121504 | H4-16 | H4 histone 16 | NM_175054.2 | 80.5% | 100% | (many diffs) |
| 15 | mouse | 326619 | Hist1h4a | histone cluster 1, H4a | NM_178192.2 | 83.8% | 100% | (many diffs) |
| 16 | mouse | 326620 | Hist1h4b | histone cluster 1, H4b | NM_178193.2 | 83.8% | 100% | (many diffs) |
| 17 | mouse | 100041230 | Hist1h4m | histone cluster 1, H4m | NM_001195421.1 | 83.4% | 100% | (many diffs) |
| 18 | mouse | 319156 | Hist1h4d | histone cluster 1, H4d | NM_175654.2 | 83.4% | 100% | (many diffs) |
| 19 | mouse | 319161 | Hist1h4n | histone cluster 1, H4n | NM_175657.2 | 83.4% | 100% | (many diffs) |
| 20 | mouse | 97122 | Hist2h4 | histone cluster 2, H4 | NM_033596.3 | 83.1% | 100% | (many diffs) |
| 21 | mouse | 319155 | Hist1h4c | histone cluster 1, H4c | NM_178208.2 | 82.9% | 100% | (many diffs) |
| 22 | mouse | 319159 | Hist1h4j | histone cluster 1, H4j | NM_178210.2 | 82.8% | 100% | (many diffs) |
| 23 | mouse | 319160 | Hist1h4k | histone cluster 1, H4k | NM_178211.2 | 82.8% | 100% | (many diffs) |
| 24 | mouse | 319157 | Hist1h4f | histone cluster 1, H4f | NM_175655.2 | 82.5% | 100% | (many diffs) |
| 25 | mouse | 319158 | Hist1h4i | histone cluster 1, H4i | NM_175656.3 | 82.5% | 100% | (many diffs) |
| 26 | mouse | 69386 | Hist1h4h | histone cluster 1, H4h | NM_153173.4 | 82.2% | 100% | (many diffs) |
| 27 | mouse | 320332 | Hist4h4 | histone cluster 4, H4 | NM_175652.3 | 81.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 378
- ORF length:
- 309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctggccgc ggcaaaggtg gaaaaggttt gggtaaggga ggggctaagc 121 gtcatcgcaa ggttttgcgc gataacatcc agggcatcac taagccagct atccggcgcc 181 ttgctcgtcg cggcggtgtc aagcgaattt ctggccttat ctatgaggag actcgcggtg 241 ttctgaaggt gttcctggag aacgtgattc gtgacgctgt cacttacaca gaacacgcca 301 aacgcaagac cgtgacagca atggatgtgg tctacgcgct gaagcgacag ggacgcactc 361 tttacggctt cggtggcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 421 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 481 agtcgacaat caacctctgg atta