Construct: ORF ccsbBroad304_11297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003715.1_s304c1, BRDN0000403132
- Derived from:
- ccsbBroadEn_11297
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL4 (8715)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11297
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001353234.1 | 84.4% | 84.4% | 1_120del;1015_1128del |
| 2 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001282527.1 | 82.8% | 82.8% | 0_1ins123;772_885del |
| 3 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001353237.1 | 82.8% | 82.8% | 0_1ins123;772_885del |
| 4 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026055.2 | 82.8% | 82.8% | 0_1ins123;772_885del |
| 5 | human | 8715 | NOL4 | nucleolar protein 4 | XM_011526238.2 | 80.5% | 80.5% | 895_1200del |
| 6 | human | 8715 | NOL4 | nucleolar protein 4 | XM_024451279.1 | 80.5% | 80.5% | 895_1200del |
| 7 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001198548.1 | 78.7% | 78.7% | 1_342del |
| 8 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026052.2 | 75.7% | 75.6% | (many diffs) |
| 9 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001198547.1 | 74.8% | 74.8% | 1_120del;1015_1320del |
| 10 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001198546.1 | 73.5% | 73.5% | 1_342del;1237_1350del |
| 11 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001353235.1 | 72.7% | 72.7% | 0_1ins123;772_1077del |
| 12 | human | 8715 | NOL4 | nucleolar protein 4 | XM_011526239.3 | 72.7% | 72.7% | 0_1ins123;772_1077del |
| 13 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026053.2 | 72.7% | 72.7% | 0_1ins123;772_1077del |
| 14 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026054.2 | 72.7% | 72.7% | 0_1ins123;772_1077del |
| 15 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026058.2 | 72.7% | 72.7% | 0_1ins123;772_1077del |
| 16 | human | 8715 | NOL4 | nucleolar protein 4 | NM_003787.4 | 66.1% | 66.1% | 1_342del;1237_1542del |
| 17 | human | 8715 | NOL4 | nucleolar protein 4 | XM_006722563.3 | 66.1% | 66.1% | 1_342del;1237_1542del |
| 18 | human | 8715 | NOL4 | nucleolar protein 4 | XM_011526237.2 | 66.1% | 66.1% | 1_342del;1237_1542del |
| 19 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026060.2 | 61% | 60.3% | (many diffs) |
| 20 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001353232.1 | 59.5% | 58.8% | (many diffs) |
| 21 | human | 8715 | NOL4 | nucleolar protein 4 | XR_935261.3 | 56.6% | (many diffs) | |
| 22 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001353236.1 | 54.5% | 54.5% | 0_1ins513;382_495del |
| 23 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001353233.1 | 53.6% | 53% | (many diffs) |
| 24 | human | 8715 | NOL4 | nucleolar protein 4 | NM_001198549.1 | 47.9% | 47.9% | 0_1ins513;382_687del |
| 25 | human | 8715 | NOL4 | nucleolar protein 4 | XM_017026059.2 | 47.9% | 47.9% | 0_1ins513;382_687del |
| 26 | human | 8715 | NOL4 | nucleolar protein 4 | XR_002958188.1 | 32.3% | (many diffs) | |
| 27 | mouse | 319211 | Nol4 | nucleolar protein 4 | XM_017317922.1 | 72.8% | 76.3% | (many diffs) |
| 28 | mouse | 319211 | Nol4 | nucleolar protein 4 | XM_017317923.1 | 72.8% | 76.3% | (many diffs) |
| 29 | mouse | 319211 | Nol4 | nucleolar protein 4 | XM_006525999.2 | 71.2% | 74.8% | (many diffs) |
| 30 | mouse | 319211 | Nol4 | nucleolar protein 4 | NM_001161483.1 | 66% | 69.3% | (many diffs) |
| 31 | mouse | 319211 | Nol4 | nucleolar protein 4 | NM_001347510.1 | 65% | 68.2% | (many diffs) |
| 32 | mouse | 319211 | Nol4 | nucleolar protein 4 | XM_017317921.1 | 63.9% | 66.9% | (many diffs) |
| 33 | mouse | 319211 | Nol4 | nucleolar protein 4 | NM_001347509.1 | 58.5% | 61.4% | (many diffs) |
| 34 | mouse | 319211 | Nol4 | nucleolar protein 4 | NM_199024.2 | 51.1% | 52.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1332
- ORF length:
- 1266
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tgtggaaacg gggccaaatg gagaacaaat tcggaaacac gctggacaaa 121 agagaactta caaagcaatt tcagagagct atgccttcct accaagagaa gcggtgacac 181 gatttctaat gagctgctca gagtgccaga aaagaatgca tttaaaccca gatggaacag 241 atcataaaga taatggaaaa cctcccactt tggtgaccag catgattgac tacaacatgc 301 caattaccat ggcctacatg aaacacatga agctgcagct gctaaactca cagcaagatg 361 aggatgaaag ttcaatagaa agtgatgaat ttgacatgag tgattcaaca cggatgtcag 421 ctgtgaactc tgatcttagc tccaatcttg aagaaagaat gcaaagtccc cagaatcttc 481 atggccagca agatgatgat tctgctgcag agagctttaa tggcaatgag actctggggc 541 acagttcaat tgcttcaggg ggaacacaca gcagggagat gggagactcc aacagtgatg 601 gcaaaactgg gctggagcaa gatgaacagc cactgaacct gagtgacagt cccctctctg 661 cgcagctaac ttcggaatac agaatagatg atcacaacag taatgggaaa aacaagtata 721 agaatcttct aatttctgac ctcaagatgg aacgagaggc gagagaaaat ggaagcaagt 781 ctcctgcaca tagttactcc agctatgact ctggcaaaaa tgagagtgta gaccgaggag 841 ctgaggacct ctcactaaac aggggagatg aggacgaaga tgaccacgag gaccatgacg 901 attcggagaa agttaatgag acagacggcg ttgaagccga gcggctgaaa gcttttaatg 961 atgagtctgc tccagctgac aaacagtgta aaccagaggc gacccaggcc acttactcaa 1021 catcagctgt tccaggctca caggacgtgc tgtacatcaa tggaaatggg acctatagtt 1081 accatagtta cagagggcta ggagggggtc tgctaaatct gaatgatgct tccagcagtg 1141 gacccactga tctcagcatg aagagacaat tggcgactag ctcaggatcc tccagcagct 1201 caaactccag accccagctg agtccaactg aaatcaatgc cgtgagacag cttgttgcag 1261 gatatcgaga atcagctgca tttttattgc gatctgcaga tgaactggaa aatctcattt 1321 tacaacagaa ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1381 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1441 caatcaacct ctggatta