Construct: ORF ccsbBroad304_11305

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008821.1_s304c1, BRDN0000410721
Derived from:
ccsbBroadEn_11305
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SUCLG2 (8801)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11305
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8801 SUCLG2 succinate-CoA ligase GDP-fo... NM_003848.3 88.8% 88.8% 1_144del
2 human 8801 SUCLG2 succinate-CoA ligase GDP-fo... NM_001177599.1 82% 80.3% (many diffs)
3 human 8801 SUCLG2 succinate-CoA ligase GDP-fo... XM_017007420.2 81.6% 80.6% (many diffs)
4 human 8801 SUCLG2 succinate-CoA ligase GDP-fo... XR_001740348.2 45.7% 1_169del;1088_1257del;1492_2519del
5 human 8801 SUCLG2 succinate-CoA ligase GDP-fo... XR_001740350.2 21.7% (many diffs)
6 mouse 20917 Suclg2 succinate-Coenzyme A ligase... NM_001326558.1 88.5% 94.5% (many diffs)
7 mouse 20917 Suclg2 succinate-Coenzyme A ligase... NM_011507.3 78.5% 83.8% (many diffs)
8 mouse 20917 Suclg2 succinate-Coenzyme A ligase... XM_017321481.1 54.3% 56.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1218
ORF length:
1152
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tgacaacgga gtgagagttc aaagattctt tgtagcagac actgcaaatg
121aagctctcga ggctgctaag agactaaatg caaaagaaat tgttttaaaa gcccagatct
181tagctggagg aagaggaaaa ggtgtcttca atagtggttt gaaaggaggt gttcatttaa
241caaaagaccc taatgttgtg ggacagctgg ctaaacagat gattgggtac aatctagcga
301caaaacaaac tccaaaagaa ggtgtgaaag ttaacaaggt gatggttgct gaagccttgg
361atatttccag agaaacctac ctggcaattc tgatggaccg gtcctgcaat ggccccgtgc
421tggtgggcag cccccagggg ggcgtcgaca ttgaagaggt ggctgcttca aacccggagc
481tcatttttaa ggagcaaatt gacatttttg aaggaataaa ggacagccaa gctcagcgga
541tggccgaaaa tctaggcttc gttgggcctt tgaaaagcca ggctgcagat caaattacga
601agctgtataa tctcttcctg aaaattgatg ctactcaggt ggaagtgaat ccctttggtg
661aaactccaga aggacaagtt gtctgttttg atgccaagat aaactttgat gacaacgcag
721aattccgaca aaaagacata tttgctatgg acgacaaatc agagaatgag cccattgaaa
781atgaagctgc caaatatgat ctaaaataca taggactaga tgggaacatt gcctgctttg
841tgaatggtgc tgggctcgcc atggctactt gtgatatcat tttccttaat ggtgggaagc
901cagccaactt cttggatctt ggaggtggtg taaaggaagc tcaagtatat caagcattca
961aattgctcac agctgatcct aaggttgaag ccatccttgt caatatattt ggtggtatcg
1021tcaactgtgc catcattgcc aatgggatca ccaaagcctg ccgggagcta gaactcaagg
1081tgcccctggt ggtccggctt gaaggaacca acgtccaaga ggcccagaag atactcaaca
1141acagcggact ccccattact tcagccattg acctggagga tgcagccaag aaggctgtgg
1201ccagtgtggc caagaagtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
1261accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1321agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)