Construct: ORF ccsbBroad304_11380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009502.1_s304c1, BRDN0000462236
- Derived from:
- ccsbBroadEn_11380
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPEPPS (9520)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11380
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9520 | NPEPPS | aminopeptidase puromycin se... | NM_001330257.2 | 95.6% | 95.6% | 1_120del |
| 2 | human | 9520 | NPEPPS | aminopeptidase puromycin se... | NM_006310.4 | 95.2% | 95.2% | 1_132del |
| 3 | human | 9520 | NPEPPS | aminopeptidase puromycin se... | XM_017025373.1 | 89.6% | 88.7% | 1_120del;2547_2641del;2760_2761ins80 |
| 4 | human | 9520 | NPEPPS | aminopeptidase puromycin se... | XM_017025372.2 | 89.2% | 88.3% | 1_132del;2559_2653del;2772_2773ins80 |
| 5 | human | 9520 | NPEPPS | aminopeptidase puromycin se... | XM_024451038.1 | 63.4% | 62% | 0_1ins819;1608_1702del;1821_1822ins80 |
| 6 | human | 9520 | NPEPPS | aminopeptidase puromycin se... | XM_024451039.1 | 63.4% | 62% | 0_1ins819;1608_1702del;1821_1822ins80 |
| 7 | human | 440434 | NPEPPSP1 | NPEPPS pseudogene 1 | NR_036750.2 | 44.1% | (many diffs) | |
| 8 | human | 101929950 | LOC101929950 | puromycin-sensitive aminope... | NR_164156.1 | 28.4% | (many diffs) | |
| 9 | mouse | 19155 | Npepps | aminopeptidase puromycin se... | NM_008942.2 | 88.6% | 93.1% | (many diffs) |
| 10 | mouse | 19155 | Npepps | aminopeptidase puromycin se... | XM_006532514.2 | 81.2% | 85.3% | (many diffs) |
| 11 | mouse | 19155 | Npepps | aminopeptidase puromycin se... | XM_011248802.1 | 81.2% | 85.3% | (many diffs) |
| 12 | mouse | 19155 | Npepps | aminopeptidase puromycin se... | XR_879535.2 | 54.9% | (many diffs) | |
| 13 | mouse | 19155 | Npepps | aminopeptidase puromycin se... | XM_017314341.1 | 49.6% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2694
- ORF length:
- 2625
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggagaag aggcccttcg agcggctgcc tgccgatgtc tcccccatca 121 actacagcct ttgcctcaag cccgacttgc tggacttcac cttcgagggc aagctggagg 181 ccgccgccca ggtgaggcag gcgactaatc agattgtgat gaattgtgct gatattgata 241 ttattacagc ttcatatgca ccagaaggag atgaagaaat acatgctaca ggatttaact 301 atcagaatga agatgaaaaa gtcaccttgt ctttccctag tactctgcaa acaggtacgg 361 gaaccttaaa gatagatttt gttggagagc tgaatgacaa aatgaaaggt ttctatagaa 421 gtaaatatac taccccttct ggagaggtgc gctatgctgc tgtaacacag tttgaggcta 481 ctgatgcccg aagggctttt ccttgctggg atgagcctgc tatcaaagca acttttgata 541 tctcattggt tgttcctaaa gacagagtag ctttatcaaa catgaatgta attgaccgga 601 aaccataccc tgatgatgaa aatttagtgg aagtgaagtt tgcccgcaca cctgttatgt 661 ctacatatct ggtggcattt gttgtgggtg aatatgactt tgtagaaaca aggtcaaaag 721 atggtgtgtg tgtccgtgtt tacactcctg ttggcaaagc agagcaagga aaatttgcgt 781 tagaggttgc tgctaaaacc ttgccttttt ataaggacta cttcaatgtt ccttatcctc 841 tacctaaaat tgatctcatt gctattgcag actttgcagc tggtgccatg gagaactggg 901 gccttgttac ttatagggag actgcattgc ttattgatcc aaaaaattcc tgttcttcat 961 cccgccagtg ggttgctctg gttgtgggac atgaactcgc ccatcaatgg tttggaaatc 1021 ttgttactat ggaatggtgg actcatcttt ggttaaatga aggttttgca tcctggattg 1081 aatatctgtg tgtagaccac tgcttcccag agtatgatat ttggactcag tttgtttctg 1141 ctgattacac ccgtgcccag gagcttgacg ccttagataa cagccatcct attgaagtca 1201 gtgtgggcca tccatctgag gttgatgaga tatttgatgc tatatcatat agcaaaggtg 1261 catctgtcat ccgaatgctg catgactaca ttggggataa ggactttaag aaaggaatga 1321 acatgtattt aaccaagttc caacaaaaga atgctgccac agaggatctc tgggaaagtt 1381 tagaaaatgc tagtggtaaa cctatagcag ctgtgatgaa tacctggacc aaacaaatgg 1441 gatttcccct catttatgtg gaagctgaac aggtagaaga tgacagatta ttgaggttgt 1501 cccaaaagaa gttctgtgct ggtgggtcat atgttggtga agattgtccc cagtggatgg 1561 tccctatcac aatctctact agtgaagacc ccaaccaggc caaactaaaa attctaatgg 1621 acaagccaga gatgaatgtg gttttgaaaa atgtcaaacc agaccaatgg gtgaagttaa 1681 acttaggaac agttgggttt tatcggaccc agtacagctc tgccatgctg gaaagtttat 1741 taccaggcat tcgtgacctt tctctgcccc ctgtggatcg acttggatta cagaatgacc 1801 tcttctcctt ggctcgagct ggaatcatta gcactgtaga ggttctaaaa gtcatggagg 1861 cttttgtgaa tgagcccaat tatactgtat ggagcgacct gagctgtaac ctggggattc 1921 tctcaactct cttgtcccac acagacttct atgaggaaat ccaggagttt gtgaaagatg 1981 tcttttcacc tataggggag agactgggct gggaccccaa acctggagaa ggtcatctcg 2041 atgcactcct gaggggcttg gttctgggaa aactaggaaa agcaggacat aaggcaacgt 2101 tagaagaagc ccgtcgtcgg tttaaggacc acgtggaagg aaaacagatt ctctccgctg 2161 atctgaggag tcctgtctat ctgactgttt tgaagcatgg tgatggcact actttagata 2221 ttatgttaaa acttcataaa caagcagata tgcaagaaga gaaaaaccga atcgaaagag 2281 tccttggcgc tactcttttg cctgacctga ttcaaaaagt cctcacgttt gcactttcag 2341 aagaggtacg tccacaggac actgtatcgg taattggtgg agtagctgga ggcagcaagc 2401 atggtaggaa agctgcttgg aaattcataa aggacaactg ggaagaactt tataaccgat 2461 accagggagg attcttaata tccagactaa taaagctatc agttgaggga tttgcagttg 2521 ataaaatggc tggagaggtt aaggctttct tcgagagtca cccagctcct tcagctgagc 2581 gtaccatcca gcagtgttgt gaaaatattc tgctgaatgc tgcctggcta aagcgagatg 2641 ctgagagcat ccaccagtac ctccttcagc ggaaggcctc accacccaca gtgttgccaa 2701 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2761 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta